LOCUS       Exported                4760 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid for the insect cell expression system, two promoters, 
            Multiplication Module M, Tn7 transposition.
ACCESSION   .
VERSION     .
KEYWORDS    pKDM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, 
            Berger I
  TITLE     Protein complex expression by using multigene baculoviral vectors.
  JOURNAL   Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
  PUBMED    17117155
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     mobile_element  74..239
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    268..402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(292..311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     346..365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(661..752)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(719..736)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        797..906
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(998..1017)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1042..1090
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        1607..1635
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1824..2357
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  2424..2648
                     /mobile_element_type="transposon:Tn7"
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(2782..2799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2953..3541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3033..3052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(3652..3671)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3663..4478
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4544..4563
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gaagatgacg gtttgtcaca tggagttggc aggatgtttg attaaaaaca taacaggaag
       61 aaaaatgccc cgctgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt
      121 ttttaaggat tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa
      181 gctaatacga aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttc
      241 ggatctccta ggctcaagca gtgatcagat ccagacatga taagatacat tgatgagttt
      301 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      361 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      421 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      481 tacaaatgtg gtatggctga ttatgatcct ctagtacttc tcgacaagct tgtcgagact
      541 gcaggctcta gattcgaaag cggccgcgac tagtgagctc gtcgacgtag gcctttgaat
      601 tccgcgcgct tcggaccggg atccgcgccc gatggtggga cggtatgaat aatccggaat
      661 atttataggt ttttttatta caaaactgtt acgaaaacag taaaatactt atttatttgc
      721 gagatggtta tcattttaat tatctccatg atctattaat attccggagt aggtcgcgaa
      781 tcgatactag tatacggacc tttaattcaa cccaacacaa tatattatag ttaaataaga
      841 attattatca aatcatttgt atattaatta aaatactata ctgtaaatta cattttattt
      901 acaatcactc gacgaagact tgatcacccg ggatctcgag ccatggtgct agcagctgat
      961 gcatagcatg cggtaccggg agatggggga ggctaactga aacacggaag gagacaatac
     1021 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
     1081 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     1141 agaccccatt gggaccaata cgcccgcgtt tcttcctttt ccccacccca acccccaagt
     1201 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caagccctgc catagccagc
     1261 tttgtttaaa caaagctgta gccaaccact agaactatag ctagagtcct gggcgaacaa
     1321 acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg tcagcaccac
     1381 cggcaagcgc cgcgacggcc gaggtctacc gatctcctga agcaagggca gatccgtgca
     1441 cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg tggagaccga
     1501 aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
     1561 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagttga cataagcctg
     1621 ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
     1681 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
     1741 ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1801 tttgatgtta tggagcagca acgatgttac gcagcagcaa cgatgttacg cagcagggca
     1861 gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg
     1921 gccctgacca agtcaaatcc atgcgggctg ctcttgatct tttcggtcgt gagttcggag
     1981 acgtagccac ctactcccaa catcagccgg actccgatta cctcgggaac ttgctccgta
     2041 gtaagacatt catcgcgctt gctgccttcg accaagaagc ggttgttggc gctctcgcgg
     2101 cttacgttct gcccaagttt gagcagccgc gtagtgagat ctatatctat gatctcgcag
     2161 tctccggcga gcaccggagg cagggcattg ccaccgcgct catcaatctc ctcaagcatg
     2221 aggccaacgc gcttggtgct tatgtgatct acgtgcaagc agattacggt gacgatcccg
     2281 cagtggctct ctatacaaag ttgggcatac gggaagaagt gatgcacttt gatatcgacc
     2341 caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt
     2401 tcggtaaatt gtcacaacgc cgcgaatata gtctttacca tgcccttggc cacgcccctc
     2461 tttaatacga cgggcaattt gcacttcaga aaatgaagag tttgctttag ccataacaaa
     2521 agtccagtat gctttttcac agcataactg gactgatttc agtttacaac tattctgtct
     2581 agtttaagac tttattgtca tagtttagat ctattttgtt cagtttaaga ctttattgtc
     2641 cgcccacacc cgcttacgca gggcatccat ttattactca accgtaaccg attttgccag
     2701 gttacgcggc tggtctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     2761 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3601 agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     3661 ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccaacatgg
     3721 atgctgattt atatgggtat aaatgggctc gagataatgt cgggcaatca ggtgcgacaa
     3781 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     3841 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     3901 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3961 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     4021 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     4081 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     4141 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     4201 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4261 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4321 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     4381 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4441 aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attcttcacg accacgctga
     4501 tgagctttac cgcagtacgt ttggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4561 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4621 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg caaataagcg
     4681 ttgatattca gtcaattaca aacattaata acgaagagat gacagaaaaa ttttcattct
     4741 gtgacagaga aaaagtagcc
//
