LOCUS Exported 5450 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site. ACCESSION . VERSION . KEYWORDS pFL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5450) AUTHORS Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I TITLE Protein complex expression by using multigene baculoviral vectors. JOURNAL Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983. PUBMED 17117155 REFERENCE 2 (bases 1 to 5450) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5450 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 95..549 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(182..201) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 391..412 /label=F1ori-F /note="F1 origin, forward primer" promoter 575..679 /gene="bla" /label=AmpR promoter CDS 680..1540 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(898..917) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1711..2299 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2200..2219 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2453..2470 /label=L4440 /note="L4440 vector, forward primer" mobile_element 2604..2828 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2895..3428) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3617..3645) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" protein_bind 4041..4074 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 4264..4312 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 4337..4356 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" promoter complement(4448..4557) /label=p10 promoter /note="baculovirus promoter for expression in insect cells" promoter 4602..4693 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 4618..4635 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" polyA_signal 4952..5086 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4989..5008) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5043..5062 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 5196..5361 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 tcacagaatg aaaatttttc tgtcatctct tcgttattaa tgtttgtaat tgactgaata 61 tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc 121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 361 cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact ggaacaacac 421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 541 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 601 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 661 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 721 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 781 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 841 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 901 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 961 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 1021 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 1081 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 1141 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 1201 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 1261 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 1321 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 1381 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 1441 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 1501 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1561 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 1621 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 1681 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 1741 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 1801 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactattct 1861 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1921 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1981 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 2041 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2101 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 2161 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2221 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2281 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2341 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2401 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2461 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 2521 tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata 2581 aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata 2641 gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt 2701 ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt 2761 ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga 2821 ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg 2881 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc 2941 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg 3001 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg 3061 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta 3121 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg 3181 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag 3241 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga 3301 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata 3361 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctg 3421 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg 3481 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc 3541 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat 3601 acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg tttgtgcctt 3661 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt 3721 tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc 3781 ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc ttcaggagat 3841 cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg 3901 catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta gctatagttc 3961 tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga tcaacagatc 4021 ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag ttatctggtt 4081 taaacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct gcgagccctg 4141 ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg gcgtattggt 4201 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt 4261 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg 4321 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccggta 4381 ccgcatgcta tgcatcagct gctagcacca tggctcgaga tcccgggtga tcaagtcttc 4441 gtcgagtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa tatacaaatg 4501 atttgataat aattcttatt taactataat atattgtgtt gggttgaatt aaaggtccgt 4561 atactagtat cgattcgcga cctactccgg aatattaata gatcatggag ataattaaaa 4621 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa 4681 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatcccggtc 4741 cgaagcgcgc ggaattcaaa ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga 4801 atctagagcc tgcagtctcg acaagcttgt cgagaagtac tagaggatca taatcagcca 4861 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct 4921 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta 4981 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 5041 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat cactgcttga 5101 gcctagaaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg 5161 ctgagcaata actatcataa cccctaggag atccgaacca gataagtgaa atctagttcc 5221 aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc agttcccatc 5281 tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc tcccagttcc 5341 caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttt taatcaaaca 5401 tcctgccaac tccatgtgac aaaccgtcat cttcggctac tttttctctg //