LOCUS Exported 6431 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION HDAC3 KD. ACCESSION . VERSION . KEYWORDS pRNATU6.1 HDAC3 shRNA1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6431) TITLE Di Giovanni Lab neuroscience plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 6431) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6431 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 69..88 /label=T7 /note="T7 promoter, forward primer" promoter 69..87 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 218..458 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 218..238 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 389..408 /label=LKO.1 5' /note="Human U6 promoter, forward primer" primer_bind complement(557..574) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" enhancer 627..930 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 931..1134 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1085..1105 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 1179..1895 /codon_start=1 /product="Aequorea coerulescens GFP" /label=AcGFP1 /note="mammalian codon-optimized" /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG NYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFG FVTAAAITHGMDELYK" polyA_signal 1910..2031 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 2132..2560 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2219..2238) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2429..2450 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(2569..2589) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2574..2903 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2754..2889 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2816..2835 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2970..3764 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3024..3043) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3634..3653 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3938..4059 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4108..4124) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4108..4124) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4121..4143) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4132..4148 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4156..4186) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4201..4222 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4339..4356) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4510..5098) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4590..4609) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5269..6129) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5892..5911 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6130..6234) /gene="bla" /label=AmpR promoter primer_bind complement(6309..6328) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata 61 tacgcgttta atacgactca ctatagggag agagagagaa ttaccctcac taaagggagg 121 agaagcatga attccccagt ggaaagacgc gcaggcaaaa cgcaccacgt gacggagcgt 181 gaccgcgcgc cgagcgcgcg ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc 241 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt 301 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt 361 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta 421 tttcgatttc ttgggtttat atatcttgtg gaaaggacgc gggatcccgg gtgctctaca 481 ttgatatcgt tgatatccgc gatatcaatg tagagcaccc ttttttccaa aagcttaagt 541 ttaaaccgct gatcagcctc gactgtgcct tctaaatagt aatcaattac ggggtcatta 601 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 661 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 721 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 781 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 841 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 901 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 961 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 1021 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 1081 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta 1141 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat gagcaagggc gccgagctgt 1201 tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc cacaagttca 1261 gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 1321 gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg agctacggcg 1381 tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc aagagcgcca 1441 tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc aactacaagt 1501 cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag ctgaccggca 1561 ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac tacaacgccc 1621 acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac ttcaagatcc 1681 gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag aataccccca 1741 tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag agcgccctgt 1801 ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg accgccgccg 1861 ccatcaccca cggcatggat gagctgtaca agtgaggact agataactga acttgtttat 1921 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 1981 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 2041 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 2101 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 2161 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 2221 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 2281 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 2341 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 2401 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 2461 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 2521 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 2581 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2641 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2701 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 2761 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 2821 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 2881 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 2941 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3001 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3061 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3121 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 3181 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3241 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3301 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 3361 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 3421 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 3481 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 3541 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3601 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 3661 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 3721 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 3781 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 3841 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 3901 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 3961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 4021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 4081 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 4141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4921 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 4981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 5041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 5101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 5161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 5221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 6001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 6061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 6121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 6181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6241 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc 6301 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt 6361 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg 6421 accgacaatt g //