LOCUS Exported 10840 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses GFP-tagged Arkadia in mammalian cells. ACCESSION . VERSION . KEYWORDS gfp-Arkadia/RNF111 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10840) AUTHORS Mavrakis KJ, Andrew RL, Lee KL, Petropoulou C, Dixon JE, Navaratnam N, Norris DP, Episkopou V TITLE Arkadia enhances Nodal/TGF-beta signaling by coupling phospho-Smad2/3 activity and turnover. JOURNAL PLoS Biol. 2007 Mar;5(3):e67. doi: 10.1371/journal.pbio.0050067. PUBMED 17341133 REFERENCE 2 (bases 1 to 10840) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10840 /organism="synthetic DNA construct" /mol_type="other DNA" misc_recomb 1..828 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" CDS 1..125 /codon_start=3 /product="baculovirus late expression factor 2" /label=lef2 /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(163..768) /codon_start=1 /product="baculovirus ORF603 protein" /label=ORF603 /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" enhancer 960..1263 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 1916..1938 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2000..2019 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 2061..2080 /label=T7 /note="T7 promoter, forward primer" promoter 2061..2079 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 2080..2104 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 2120..2229 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" CDS 2250..2267 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 2277..2321 /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /label=S-Tag /translation="KETAAAKFERQHMDS" regulatory 2349..2358 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2355..3071 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2355..3071 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2400..2421) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2661..2680) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3008..3029 /label=EGFP-C /note="EGFP, forward primer" CDS 4599..4619 /codon_start=1 /product="6xHis affinity tag" /label=7xHis /translation="HHHHHHH" CDS 6193..6216 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" misc_feature 6270..6732 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6326..6343) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" CDS 6771..7790 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE" primer_bind complement(7955..7974) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 8020..8075 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(8074..8093) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" terminator 8163..8210 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_recomb 8223..8928 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" 3'UTR 8311..8683 /label=polyhedrin 3' UTR /note="3' UTR from the Autographa californica nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)" CDS complement(8340..8928) /codon_start=2 /product="baculovirus capsid-associated protein" /label=ORF1629 /note="required for viral replication" /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" protein_bind 8937..8970 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." rep_origin complement(9138..9725) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9217..9236) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9738..10598) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10361..10380 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 61 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 121 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 181 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 241 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 301 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 361 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 421 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 481 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 541 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 601 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 661 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 721 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 781 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg 841 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct 901 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 961 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 1021 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 1081 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 1141 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 1201 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 1261 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 1321 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 1381 gggggggggg nngngsgssr ggsggggngg gggggggggg gggggggggg gggggggggg 1441 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg 1501 gggggggggg ggggsggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg 1561 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc 1621 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg 1681 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc 1741 gggacggccc ttctccttcg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt 1801 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg 1861 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc 1921 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc 1981 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa 2041 gaattggatc ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa 2101 ttccccggag ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata 2161 agaattatta tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta 2221 tttacaatca aaggagatat accatggcac accatcacca ccatcactct tctggtaaag 2281 aaaccgctgc tgcgaaattt gaacgccagc acatggactc gccaccgcct tctggtctgg 2341 tccccctcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 2401 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 2461 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 2521 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 2581 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 2641 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 2701 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 2761 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 2821 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 2881 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 2941 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 3001 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 3061 agctgtacaa gtccggactc agatctaagc ttgagctcta caccctaaaa gtggctatga 3121 agagtgggac tcctgatgcg cccactacac aggagagtct gaaggcggtc cttctgcacc 3181 cacagcccct aggggccacc aagagcttcc ctgcagaagt ggagatgatt aatagcaaag 3241 tggggaatga attctctcat ctgtgtgatg attctcaaaa acaagagaag gacatgactg 3301 gcaaccagca agaacaagaa aaaagtggtg ttgtgagaaa aaagcgcaaa agccagcagg 3361 ctggcccctc atatgtgcag aactgtgtca aagaaaatca ggaaatccta gggcgcagac 3421 aacagctaga gacaccgagt gatgaggata atgactcttc cttaagtgag tgtctgtctt 3481 ctccctcatc tagtctccat tttgggggct ctgatacagt gacttcagat gaggataaag 3541 aagtctcggt gagacatacc cagcctgttc tgagtgctaa aagtagaagt cacagtgcaa 3601 ggtctcacaa gtggcctcga actgaggcag atcctgtgcc aagtttgtta atgaaaagac 3661 cttgttttca tggcagtgca ctaaggagag tcacatgcag aaagaggctg gtgaagagca 3721 gctcctcgca gaggacacag aagcagaagg agaggatgct agtgcagagg aagaagcggg 3781 aggcgctagc tcagaggaag tatgctcttc tctccagctc cagtagctcc agtgagaatg 3841 acctcagcag cgattcctcg tccagctcct ccaccgatgg agaagaggac ctgtgtgcat 3901 ctgccagtga gaaccccagc aaccccgctg ctccctcagg aagtattgat gaagatgttg 3961 tggtgataga agcctccttc actccccagg tcactgccaa tgaagaaatt aatgttacct 4021 caactgacag tgaagtggag attgttacag ttggagaaag ctatcggtct cgttcaaccc 4081 ttggacactc cagatctcat tggagccaag gttcaagttc gcatacagga cggccacagg 4141 agtctcgaaa ccgcagtagg atctctactg ttatacagcc cctgaggcag aatgcagcag 4201 aagttgtgga ccttactgtt gatgaagatg aacctactat agtaccaacc acttctgcta 4261 gaatggactc acaaactact agtgcttcca ttaacaattc aaatccatct acctctgagc 4321 aagcctctga tactacttca actgtggcca gtagccagcc ttccacagtg tcagagactg 4381 aagctactct tacaagcaat agtgcaactg gttcttctgt gggcgatgac gtgaggagaa 4441 ctgcatctag tgctgtccca gaaagtggtc ctcctgcaat gccaaggcta ccttcttgct 4501 gcccccagca ctcaccatgt ggagggacat cacagagcca tcatgcatta gcacacccac 4561 actcaagttg ttttcagcag catggccacc attttcagca tcaccaccat caccaccata 4621 ctccccaccc agctgtccca gtttctcctt ccttcagtga ccctgcttgc ccagtggaaa 4681 gacctcaagt acaggcgcct tgtggagcaa atagcagctc tggttccagc taccatgacc 4741 agcaggcatt gccagtcgat ctgagcaaca gtgctctcag gactcatgga agtgggggtt 4801 tccatggtgc atctgctttt gacccctgct gccctgtcac atcttcccgg gctgcagtct 4861 ttggccatca ggctgctgct gccccgactc agccattatc aatagatggc tatggctcca 4921 gcatggttgc ccagccccag ccccagcccc ctccacagcc ttctctttcg tcatgtcggc 4981 actacatgcc acctccgtat gcttccttga caagaccact gcaccatcaa gcctcagctt 5041 gccatcactc tcatggaaat gcccctcccc agactcagcc tccccctcaa gtggattacg 5101 tgattccgca tcctgtccat gctttccatt ctcagatatc ttctcatgca gcatctcacc 5161 ctgtggcacc accgccgcca acacatttag gcagtacagc ggcacccatc cctcagcatc 5221 ttcctcctgc tcaccagccg atttcacacc atattccagc cccagcacct tcagcccaga 5281 gactgcaccc tcatgaagtg atgcagagga tggaagttca gaggaggagg atgatgcagc 5341 atccaacgcg ggcacatgaa cggcccccgc cccatccaca taggatgcac ccaaactatg 5401 gccatgggca tcatattcat gtgcctcaaa ccatgtcttc acatcctcgc caggctccag 5461 agagaactgc ctgggaactg gggattgaag ctggagtaac tgcagctacc tacacacctg 5521 gtgctctgca ccctcacctg gcccattacc acgccccgcc tcgacttcat cacctccagt 5581 taggagcact tcctttaatg gttcctgaca tggcaggcta tcctcacatc cgttatattt 5641 cttcaggatt ggatggagca tcattcagag gtccttttag gggcaatttt gaggaattga 5701 ttcatctgga agaaagatta ggaaatgtca atcgtggagc ctcccaggga acaattgaaa 5761 gatgcacata tccacataaa tataaaaagg taacaactga ttggttctca cagaggaaac 5821 tgcactgcaa acaagatggg gaagaaggga ctgaggaaga cacagaggaa aaatgtacta 5881 tctgtttgtc tattttagag gaaggtgaag atgtgaggcg ccttccatgt atgcaccttt 5941 tccaccaagt gtgtgttgat cagtggctga tcaccaataa gaagtgcccc atatgcagag 6001 tggacatcga ggcccagctg ccaagtgaaa gttgacattg tgtttcagaa ctcttggtgt 6061 ccctctcatt cctatccttc ctggtactgc agtcaaccaa agatggcatg acttacctgc 6121 gcagatctgt catgatgatc attgcaattg gatccatata tagggcccgg gttataatta 6181 cctcaggtcg agcaccacca tcaccatcac catcactaag tgattaacct caggtgcagg 6241 ctgcctatca gaaggtggtg gctggtgtgg agggcccgga aacctggccc tgtcttcttg 6301 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 6361 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 6421 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 6481 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 6541 gaaagagtca aatggctcac ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 6601 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 6661 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa 6721 acacgatgat aagcttgcca caacccgtac caaagatgga tagatccgga aagcctgaac 6781 tcaccgcgac gtctgttgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga 6841 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat 6901 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc 6961 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa tttagcgaga 7021 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa 7081 ccgaactgcc cgctgttctg caaccggtcg cggaggctat ggatgcgatc gctgcggccg 7141 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta 7201 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga 7261 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg 7321 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga 7381 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc 7441 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga 7501 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata 7561 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg 7621 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc 7681 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg 7741 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc 7801 gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag 7861 gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaac 7921 caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc aaaaattatg 7981 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca 8041 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa 8101 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgt 8161 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcat 8221 gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac 8281 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat 8341 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat 8401 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg 8461 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta 8521 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc 8581 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt 8641 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt 8701 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca 8761 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg 8821 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg 8881 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc gatcgcataa 8941 cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg 9001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 9061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 9121 gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac aaaaatcgac 9181 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 9241 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 9301 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 9361 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 9421 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 9481 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 9541 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 9601 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 9661 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 9721 ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 9781 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 9841 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 9901 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 9961 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 10021 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 10081 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 10141 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 10201 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 10261 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 10321 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 10381 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 10441 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 10501 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 10561 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 10621 tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt tcctgcatct tttaatcaaa 10681 tcccaagatg tgtataaacg cgccggtatg tacaggaaga ggtttatact aaactgttac 10741 attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat gtttaatcaa ggctctgacg 10801 catttctaca accacgactc caagtgtgtg ggtgaagtca //