LOCUS       Exported                8442 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses hA3A-BE3-C101S in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-BE3-C101S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             409..1005
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPSFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             1054..5154
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5167..5415
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5428..5448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5457..5474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5497..5514)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5503..5727
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5798..5814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5798..5814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5811..5833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5822..5838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5846..5876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5891..5912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6029..6046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6200..6788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6280..6299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6959..7819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7582..7601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7820..7924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8003..8022)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8194..131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggaagccagc
      421 ccagcatccg ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat
      481 ggcattggaa ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc
      541 tcggtcaaga tggaccagca caggggcttt ctacacaacc aggctaagaa tcttctctgt
      601 ggcttttacg gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg
      661 gacccggccc agatctacag ggtcacttgg ttcatctcct ggagccccag cttctcctgg
      721 ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgtatc
      781 ttcgctgccc gcatctatga ttacgacccc ctatataagg aggcactgca aatgctgcgg
      841 gatgctgggg cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc
      901 tttgtggacc accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa
      961 gccctgagtg ggaggctgcg ggccattctc cagaatcagg gaaacagcgg cagcgagact
     1021 cccgggacct cagagtccgc cacacccgaa agtgataaaa agtattctat tggtttagcc
     1081 atcggcacta attccgttgg atgggctgtc ataaccgatg aatacaaagt accttcaaag
     1141 aaatttaagg tgttggggaa cacagaccgt cattcgatta aaaagaatct tatcggtgcc
     1201 ctcctattcg atagtggcga aacggcagag gcgactcgcc tgaaacgaac cgctcggaga
     1261 aggtatacac gtcgcaagaa ccgaatatgt tacttacaag aaatttttag caatgagatg
     1321 gccaaagttg acgattcttt ctttcaccgt ttggaagagt ccttccttgt cgaagaggac
     1381 aagaaacatg aacggcaccc catctttgga aacatagtag atgaggtggc atatcatgaa
     1441 aagtacccaa cgatttatca cctcagaaaa aagctagttg actcaactga taaagcggac
     1501 ctgaggttaa tctacttggc tcttgcccat atgataaagt tccgtgggca ctttctcatt
     1561 gagggtgatc taaatccgga caactcggat gtcgacaaac tgttcatcca gttagtacaa
     1621 acctataatc agttgtttga agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct
     1681 attcttagcg cccgcctctc taaatcccga cggctagaaa acctgatcgc acaattaccc
     1741 ggagagaaga aaaatgggtt gttcggtaac cttatagcgc tctcactagg cctgacacca
     1801 aattttaagt cgaacttcga cttagctgaa gatgccaaat tgcagcttag taaggacacg
     1861 tacgatgacg atctcgacaa tctactggca caaattggag atcagtatgc ggacttattt
     1921 ttggctgcca aaaaccttag cgatgcaatc ctcctatctg acatactgag agttaatact
     1981 gagattacca aggcgccgtt atccgcttca atgatcaaaa ggtacgatga acatcaccaa
     2041 gacttgacac ttctcaaggc cctagtccgt cagcaactgc ctgagaaata taaggaaata
     2101 ttctttgatc agtcgaaaaa cgggtacgca ggttatattg acggcggagc gagtcaagag
     2161 gaattctaca agtttatcaa acccatatta gagaagatgg atgggacgga agagttgctt
     2221 gtaaaactca atcgcgaaga tctactgcga aagcagcgga ctttcgacaa cggtagcatt
     2281 ccacatcaaa tccacttagg cgaattgcat gctatactta gaaggcagga ggatttttat
     2341 ccgttcctca aagacaatcg tgaaaagatt gagaaaatcc taacctttcg cataccttac
     2401 tatgtgggac ccctggcccg agggaactct cggttcgcat ggatgacaag aaagtccgaa
     2461 gaaacgatta ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg
     2521 ttcatcgaga ggatgaccaa ctttgacaag aatttaccga acgaaaaagt attgcctaag
     2581 cacagtttac tttacgagta tttcacagtg tacaatgaac tcacgaaagt taagtatgtc
     2641 actgagggca tgcgtaaacc cgcctttcta agcggagaac agaagaaagc aatagtagat
     2701 ctgttattca agaccaaccg caaagtgaca gttaagcaat tgaaagagga ctactttaag
     2761 aaaattgaat gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca
     2821 cttggtacgt atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa
     2881 gagaatgaag atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa
     2941 atgattgagg aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag
     3001 ttaaagaggc gtcgctatac gggctgggga cgattgtcgc ggaaacttat caacgggata
     3061 agagacaagc aaagtggtaa aactattctc gattttctaa agagcgacgg cttcgccaat
     3121 aggaacttta tgcagctgat ccatgatgac tctttaacct tcaaagagga tatacaaaag
     3181 gcacaggttt ccggacaagg ggactcattg cacgaacata ttgcgaatct tgctggttcg
     3241 ccagccatca aaaagggcat actccagaca gtcaaagtag tggatgagct agttaaggtc
     3301 atgggacgtc acaaaccgga aaacattgta atcgagatgg cacgcgaaaa tcaaacgact
     3361 cagaaggggc aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg tattaaagaa
     3421 ctgggcagcc agatcttaaa ggagcatcct gtggaaaata cccaattgca gaacgagaaa
     3481 ctttacctct attacctaca aaatggaagg gacatgtatg ttgatcagga actggacata
     3541 aaccgtttat ctgattacga cgtcgatcac attgtacccc aatccttttt gaaggacgat
     3601 tcaatcgaca ataaagtgct tacacgctcg gataagaacc gagggaaaag tgacaatgtt
     3661 ccaagcgagg aagtcgtaaa gaaaatgaag aactattggc ggcagctcct aaatgcgaaa
     3721 ctgataacgc aaagaaagtt cgataactta actaaagctg agaggggtgg cttgtctgaa
     3781 cttgacaagg ccggatttat taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat
     3841 gttgcacaga tactagattc ccgaatgaat acgaaatacg acgagaacga taagctgatt
     3901 cgggaagtca aagtaatcac tttaaagtca aaattggtgt cggacttcag aaaggatttt
     3961 caattctata aagttaggga gataaataac taccaccatg cgcacgacgc ttatcttaat
     4021 gccgtcgtag ggaccgcact cattaagaaa tacccgaagc tagaaagtga gtttgtgtat
     4081 ggtgattaca aagtttatga cgtccgtaag atgatcgcga aaagcgaaca ggagataggc
     4141 aaggctacag ccaaatactt cttttattct aacattatga atttctttaa gacggaaatc
     4201 actctggcaa acggagagat acgcaaacga cctttaattg aaaccaatgg ggagacaggt
     4261 gaaatcgtat gggataaggg ccgggacttc gcgacggtga gaaaagtttt gtccatgccc
     4321 caagtcaaca tagtaaagaa aactgaggtg cagaccggag ggttttcaaa ggaatcgatt
     4381 cttccaaaaa ggaatagtga taagctcatc gctcgtaaaa aggactggga cccgaaaaag
     4441 tacggtggct tcgatagccc tacagttgcc tattctgtcc tagtagtggc aaaagttgag
     4501 aagggaaaat ccaagaaact gaagtcagtc aaagaattat tggggataac gattatggag
     4561 cgctcgtctt ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta caaggaagta
     4621 aaaaaggatc tcataattaa actaccaaag tatagtctgt ttgagttaga aaatggccga
     4681 aaacggatgt tggctagcgc cggagagctt caaaagggga acgaactcgc actaccgtct
     4741 aaatacgtga atttcctgta tttagcgtcc cattacgaga agttgaaagg ttcacctgaa
     4801 gataacgaac agaagcaact ttttgttgag cagcacaaac attatctcga cgaaatcata
     4861 gagcaaattt cggaattcag taagagagtc atcctagctg atgccaatct ggacaaagta
     4921 ttaagcgcat acaacaagca cagggataaa cccatacgtg agcaggcgga aaatattatc
     4981 catttgttta ctcttaccaa cctcggcgct ccagccgcat tcaagtattt tgacacaacg
     5041 atagatcgca aacgatacac ttctaccaag gaggtgctag acgcgacact gattcaccaa
     5101 tccatcacgg gattatatga aactcggata gatttgtcac agcttggggg tgactctggt
     5161 ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact ggttatccag
     5221 gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa gccggaaagc
     5281 gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat gcttctgact
     5341 agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa cggtgagaac
     5401 aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcta accggtcatc
     5461 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     5521 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     5581 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5641 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5701 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     5761 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5821 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5881 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5941 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     7981 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     8041 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8101 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     8161 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     8221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     8281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     8341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     8401 atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
