LOCUS       Exported                9409 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses hA3A-eBE-Y132D in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-eBE-Y132D
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             409..1005
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDDDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             1054..5154
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5167..5415
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5428..5448
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5455..5508
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5509..5757
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5758..5778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5779..5835
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5836..6084
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6085..6105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6106..6159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6160..6408
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6421..6441
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6464..6481)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6470..6694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6765..6781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6765..6781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6778..6800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6789..6805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6813..6843)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6858..6879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6996..7013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7167..7755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7247..7266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7926..8786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8549..8568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8787..8891)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8970..8989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9161..131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggaagccagc
      421 ccagcatccg ggcccagaca cttgatggat ccacacatat tcacttccaa ctttaacaat
      481 ggcattggaa ggcataagac ctacctgtgc tacgaagtgg agcgcctgga caatggcacc
      541 tcggtcaaga tggaccagca caggggcttt ctacacaacc aggctaagaa tcttctctgt
      601 ggcttttacg gccgccatgc ggagctgcgc ttcttggacc tggttccttc tttgcagttg
      661 gacccggccc agatctacag ggtcacttgg ttcatctcct ggagcccctg cttctcctgg
      721 ggctgtgccg gggaagtgcg tgcgttcctt caggagaaca cacacgtgag actgcgtatc
      781 ttcgctgccc gcatctatga tgatgacccc ctatataagg aggcactgca aatgctgcgg
      841 gatgctgggg cccaagtctc catcatgacc tacgatgaat ttaagcactg ctgggacacc
      901 tttgtggacc accagggatg tcccttccag ccctgggatg gactagatga gcacagccaa
      961 gccctgagtg ggaggctgcg ggccattctc cagaatcagg gaaacagcgg cagcgagact
     1021 cccgggacct cagagtccgc cacacccgaa agtgataaaa agtattctat tggtttagcc
     1081 atcggcacta attccgttgg atgggctgtc ataaccgatg aatacaaagt accttcaaag
     1141 aaatttaagg tgttggggaa cacagaccgt cattcgatta aaaagaatct tatcggtgcc
     1201 ctcctattcg atagtggcga aacggcagag gcgactcgcc tgaaacgaac cgctcggaga
     1261 aggtatacac gtcgcaagaa ccgaatatgt tacttacaag aaatttttag caatgagatg
     1321 gccaaagttg acgattcttt ctttcaccgt ttggaagagt ccttccttgt cgaagaggac
     1381 aagaaacatg aacggcaccc catctttgga aacatagtag atgaggtggc atatcatgaa
     1441 aagtacccaa cgatttatca cctcagaaaa aagctagttg actcaactga taaagcggac
     1501 ctgaggttaa tctacttggc tcttgcccat atgataaagt tccgtgggca ctttctcatt
     1561 gagggtgatc taaatccgga caactcggat gtcgacaaac tgttcatcca gttagtacaa
     1621 acctataatc agttgtttga agagaaccct ataaatgcaa gtggcgtgga tgcgaaggct
     1681 attcttagcg cccgcctctc taaatcccga cggctagaaa acctgatcgc acaattaccc
     1741 ggagagaaga aaaatgggtt gttcggtaac cttatagcgc tctcactagg cctgacacca
     1801 aattttaagt cgaacttcga cttagctgaa gatgccaaat tgcagcttag taaggacacg
     1861 tacgatgacg atctcgacaa tctactggca caaattggag atcagtatgc ggacttattt
     1921 ttggctgcca aaaaccttag cgatgcaatc ctcctatctg acatactgag agttaatact
     1981 gagattacca aggcgccgtt atccgcttca atgatcaaaa ggtacgatga acatcaccaa
     2041 gacttgacac ttctcaaggc cctagtccgt cagcaactgc ctgagaaata taaggaaata
     2101 ttctttgatc agtcgaaaaa cgggtacgca ggttatattg acggcggagc gagtcaagag
     2161 gaattctaca agtttatcaa acccatatta gagaagatgg atgggacgga agagttgctt
     2221 gtaaaactca atcgcgaaga tctactgcga aagcagcgga ctttcgacaa cggtagcatt
     2281 ccacatcaaa tccacttagg cgaattgcat gctatactta gaaggcagga ggatttttat
     2341 ccgttcctca aagacaatcg tgaaaagatt gagaaaatcc taacctttcg cataccttac
     2401 tatgtgggac ccctggcccg agggaactct cggttcgcat ggatgacaag aaagtccgaa
     2461 gaaacgatta ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc agctcaatcg
     2521 ttcatcgaga ggatgaccaa ctttgacaag aatttaccga acgaaaaagt attgcctaag
     2581 cacagtttac tttacgagta tttcacagtg tacaatgaac tcacgaaagt taagtatgtc
     2641 actgagggca tgcgtaaacc cgcctttcta agcggagaac agaagaaagc aatagtagat
     2701 ctgttattca agaccaaccg caaagtgaca gttaagcaat tgaaagagga ctactttaag
     2761 aaaattgaat gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca
     2821 cttggtacgt atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa
     2881 gagaatgaag atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa
     2941 atgattgagg aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag
     3001 ttaaagaggc gtcgctatac gggctgggga cgattgtcgc ggaaacttat caacgggata
     3061 agagacaagc aaagtggtaa aactattctc gattttctaa agagcgacgg cttcgccaat
     3121 aggaacttta tgcagctgat ccatgatgac tctttaacct tcaaagagga tatacaaaag
     3181 gcacaggttt ccggacaagg ggactcattg cacgaacata ttgcgaatct tgctggttcg
     3241 ccagccatca aaaagggcat actccagaca gtcaaagtag tggatgagct agttaaggtc
     3301 atgggacgtc acaaaccgga aaacattgta atcgagatgg cacgcgaaaa tcaaacgact
     3361 cagaaggggc aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg tattaaagaa
     3421 ctgggcagcc agatcttaaa ggagcatcct gtggaaaata cccaattgca gaacgagaaa
     3481 ctttacctct attacctaca aaatggaagg gacatgtatg ttgatcagga actggacata
     3541 aaccgtttat ctgattacga cgtcgatcac attgtacccc aatccttttt gaaggacgat
     3601 tcaatcgaca ataaagtgct tacacgctcg gataagaacc gagggaaaag tgacaatgtt
     3661 ccaagcgagg aagtcgtaaa gaaaatgaag aactattggc ggcagctcct aaatgcgaaa
     3721 ctgataacgc aaagaaagtt cgataactta actaaagctg agaggggtgg cttgtctgaa
     3781 cttgacaagg ccggatttat taaacgtcag ctcgtggaaa cccgccaaat cacaaagcat
     3841 gttgcacaga tactagattc ccgaatgaat acgaaatacg acgagaacga taagctgatt
     3901 cgggaagtca aagtaatcac tttaaagtca aaattggtgt cggacttcag aaaggatttt
     3961 caattctata aagttaggga gataaataac taccaccatg cgcacgacgc ttatcttaat
     4021 gccgtcgtag ggaccgcact cattaagaaa tacccgaagc tagaaagtga gtttgtgtat
     4081 ggtgattaca aagtttatga cgtccgtaag atgatcgcga aaagcgaaca ggagataggc
     4141 aaggctacag ccaaatactt cttttattct aacattatga atttctttaa gacggaaatc
     4201 actctggcaa acggagagat acgcaaacga cctttaattg aaaccaatgg ggagacaggt
     4261 gaaatcgtat gggataaggg ccgggacttc gcgacggtga gaaaagtttt gtccatgccc
     4321 caagtcaaca tagtaaagaa aactgaggtg cagaccggag ggttttcaaa ggaatcgatt
     4381 cttccaaaaa ggaatagtga taagctcatc gctcgtaaaa aggactggga cccgaaaaag
     4441 tacggtggct tcgatagccc tacagttgcc tattctgtcc tagtagtggc aaaagttgag
     4501 aagggaaaat ccaagaaact gaagtcagtc aaagaattat tggggataac gattatggag
     4561 cgctcgtctt ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta caaggaagta
     4621 aaaaaggatc tcataattaa actaccaaag tatagtctgt ttgagttaga aaatggccga
     4681 aaacggatgt tggctagcgc cggagagctt caaaagggga acgaactcgc actaccgtct
     4741 aaatacgtga atttcctgta tttagcgtcc cattacgaga agttgaaagg ttcacctgaa
     4801 gataacgaac agaagcaact ttttgttgag cagcacaaac attatctcga cgaaatcata
     4861 gagcaaattt cggaattcag taagagagtc atcctagctg atgccaatct ggacaaagta
     4921 ttaagcgcat acaacaagca cagggataaa cccatacgtg agcaggcgga aaatattatc
     4981 catttgttta ctcttaccaa cctcggcgct ccagccgcat tcaagtattt tgacacaacg
     5041 atagatcgca aacgatacac ttctaccaag gaggtgctag acgcgacact gattcaccaa
     5101 tccatcacgg gattatatga aactcggata gatttgtcac agcttggggg tgactctggt
     5161 ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact ggttatccag
     5221 gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa gccggaaagc
     5281 gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat gcttctgact
     5341 agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa cggtgagaac
     5401 aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcgg gcccgagggc
     5461 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaac taatctgtca
     5521 gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat cctcatgctc
     5581 ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc
     5641 gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac
     5701 aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctcccc
     5761 aagaagaaga ggaaagtcgc tactaacttc agcctgctga agcaggctgg agacgtggag
     5821 gagaaccctg gacctaccaa cctgtccgat atcattgaga aagagaccgg caaacagctg
     5881 gtgatccagg agagcatcct gatgctgccc gaagaggtgg aggaagtgat cggcaacaag
     5941 cccgagtccg acatcctggt gcacacagcc tatgatgaat ccaccgacga gaacgtgatg
     6001 ctgctgacct ccgatgctcc cgagtataaa ccctgggcac tggtgatcca ggactctaat
     6061 ggagagaaca agatcaagat gctgcccaag aagaagagga aagtcgaagg acggggctcc
     6121 ctcctgacct gtggcgatgt ggaagagaac cccggcccca caaacctcag tgacattatc
     6181 gagaaggaaa caggaaaaca gctcgtcatt caagaatcta ttcttatgtt gcctgaggaa
     6241 gtcgaagagg ttattggcaa taaacctgaa tctgatattc ttgtccatac cgcttacgat
     6301 gagtccacag atgaaaatgt tatgctgctc acatctgacg caccagagta caaaccatgg
     6361 gcgctcgtta ttcaagattc caacggcgaa aacaaaatca aaatgctttc tggtggttct
     6421 cctaaaaaaa aaagaaaggt gtgagtttaa acccgctgat cagcctcgac tgtgccttct
     6481 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6541 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     6601 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6661 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6721 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     6781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6841 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8881 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     8941 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9001 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9061 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9121 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     9181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     9241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     9301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     9361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatc
//
