LOCUS       Exported               15521 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Neo-Bam APC
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15521)
  AUTHORS   Morin PJ, Sparks AB, Korinek V, Barker N, Clevers H, Vogelstein B, 
            Kinzler KW
  TITLE     Activation of beta-catenin-Tcf signaling in colon cancer by 
            mutations in beta-catenin or APC.
  JOURNAL   Science. 1997 Mar 21. 275(5307):1787-90.
  PUBMED    9065402
REFERENCE   2  (bases 1 to 15521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15521
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          696..1268
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(10443..10462)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10508..10563
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10562..10581)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10921..10937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10921..10937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10934..10956)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10945..10961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10969..10999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11014..11035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11152..11169)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11323..11911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11403..11422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12082..12942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12705..12724
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12943..13047)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13115..13133
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13171..13193)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13293..13312
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13506..13528
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13520..13537
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13521..13537
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13948..14093
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             14150..14944
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14204..14223)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14814..14833
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(15138..15157)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15182..15229
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
      721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      841 ttcactttct actctgttga caaccattgt ctcctcttat tttcttttca ttttctgtaa
      901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     1321 tcctcgacgg atccgatcga attcccggaa atggggtcca agggtagcca aggatggctg
     1381 cagcttcata tgatcagttg ttaaagcaag ttgaggcact gaagatggag aactcaaatc
     1441 ttcgacaaga gctagaagat aattccaatc atcttacaaa actggaaact gaggcatcta
     1501 atatgaagga agtacttaaa caactacaag gaagtattga agatgaagct atggcttctt
     1561 ctggacagat tgatttatta gagcgtctta aagagcttaa cttagatagc agtaatttcc
     1621 ctggagtaaa actgcggtca aaaatgtccc tccgttctta tggaagccgg gaaggatctg
     1681 tatcaagccg ttctggagag tgcagtcctg ttcctatggg ttcatttcca agaagagggt
     1741 ttgtaaatgg aagcagagaa agtactggat atttagaaga acttgagaaa gagaggtcat
     1801 tgcttcttgc tgatcttgac aaagaagaaa aggaaaaaga ctggtattac gctcaacttc
     1861 agaatctcac taaaagaata gatagtcttc ctttaactga aaatttttcc ttacaaacag
     1921 atatgaccag aaggcaattg gaatatgaag caaggcaaat cagagttgcg atggaagaac
     1981 aactaggtac ctgccaggat atggaaaaac gagcacagcg aagaatagcc agaattcagc
     2041 aaatcgaaaa ggacatactt cgtatacgac agcttttaca gtcccaagca acagaagcag
     2101 agaggtcatc tcagaacaag catgaaaccg gctcacatga tgctgagcgg cagaatgaag
     2161 gtcaaggagt gggagaaatc aacatggcaa cttctggtaa tggtcagggt tcaactacac
     2221 gaatggacca tgaaacagcc agtgttttga gttctagtag cacacactct gcacctcgaa
     2281 ggctgacaag tcatctggga accaaggtgg aaatggtgta ttcattgttg tcaatgcttg
     2341 gtactcatga taaggatgat atgtcgcgaa ctttgctagc tatgtctagc tcccaagaca
     2401 gctgtatatc catgcgacag tctggatgtc ttcctctcct catccagctt ttacatggca
     2461 atgacaaaga ctctgtattg ttgggaaatt cccggggcag taaagaggct cgggccaggg
     2521 ccagtgcagc actccacaac atcattcact cacagcctga tgacaagaga ggcaggcgtg
     2581 aaatccgagt ccttcatctt ttggaacaga tacgcgctta ctgtgaaacc tgttgggagt
     2641 ggcaggaagc tcatgaacca ggcatggacc aggacaaaaa tccaatgcca gctcctgttg
     2701 aacatcagat ctgtcctgct gtgtgtgttc taatgaaact ttcatttgat gaagagcata
     2761 gacatgcaat gaatgaacta gggggactac aggccattgc agaattattg caagtggact
     2821 gtgaaatgta tgggcttact aatgaccact acagtattac actaagacga tatgctggaa
     2881 tggctttgac aaacttgact tttggagatg tagccaacaa ggctacgcta tgctctatga
     2941 aaggctgcat gagagcactt gtggcccaac taaaatctga aagtgaagac ttacagcagg
     3001 ttattgcgag tgttttgagg aatttgtctt ggcgagcaga tgtaaatagt aaaaagacgt
     3061 tgcgagaagt tggaagtgtg aaagcattga tggaatgtgc tttagaagtt aaaaaggaat
     3121 caaccctcaa aagcgtattg agtgccttat ggaatttgtc agcacattgc actgagaata
     3181 aagctgatat atgtgctgta gatggtgcac ttgcattttt ggttggcact cttacttacc
     3241 ggagccagac aaacacttta gccattattg aaagtggagg tgggatatta cggaatgtgt
     3301 ccagcttgat agctacaaat gaggaccaca ggcaaatcct aagagagaac aactgtctac
     3361 aaactttatt acaacactta aaatctcata gtttgacaat agtcagtaat gcatgtggaa
     3421 ctttgtggaa tctctcagca agaaatccta aagaccagga agcattatgg gacatggggg
     3481 cagttagcat gctcaagaac ctcattcatt caaagcacaa aatgattgct atgggaagtg
     3541 ctgcagcttt aaggaatctc atggcaaata ggcctgcgaa gtacaaggat gccaatatta
     3601 tgtctcctgg ctcaagcttg ccatctcttc atgttaggaa acaaaaagcc ctagaagcag
     3661 aattagatgc tcagcactta tcagaaactt ttgacaatat agacaattta agtcccaagg
     3721 catctcatcg tagtaagcag agacacaagc aaagtctcta tggtgattat gtttttgaca
     3781 ccaatcgaca tgatgataat aggtcagaca attttaatac tggcaacatg actgtccttt
     3841 caccatattt gaatactaca gtgttaccca gctcctcttc atcaagagga agcttagata
     3901 gttctcgttc tgaaaaagat agaagtttgg agagagaacg cggaattggt ctaggcaact
     3961 accatccagc aacagaaaat ccaggaactt cttcaaagcg aggtttgcag atctccacca
     4021 ctgcagccca gattgccaaa gtcatggaag aagtgtcagc cattcatacc tctcaggaag
     4081 acagaagttc tgggtctacc actgaattac attgtgtgac agatgagaga aatgcactta
     4141 gaagaagctc tgctgcccat acacattcaa acacttacaa tttcactaag tcggaaaatt
     4201 caaataggac atgttctatg ccttatgcca aattagaata caagagatct tcaaatgata
     4261 gtttaaatag tgtcagtagt agtgatggtt atggtaaaag aggtcaaatg aaaccctcga
     4321 ttgaatccta ttctgaagat gatgaaagta agttttgcag ttatggtcaa tacccagccg
     4381 acctagccca taaaatacat agtgcaaatc atatggatga taatgatgga gaactagata
     4441 caccaataaa ttatagtctt aaatattcag atgagcagtt gaactctgga aggcaaagtc
     4501 cttcacagaa tgaaagatgg gcaagaccca aacacataat agaagatgaa ataaaacaaa
     4561 gtgagcaaag acaatcaagg aatcaaagta caacttatcc tgtttatact gagagcactg
     4621 atgataaaca cctcaagttc caaccacatt ttggacagca ggaatgtgtt tctccataca
     4681 ggtcacgggg agccaatggt tcagaaacaa atcgagtggg ttctaatcat ggaattaatc
     4741 aaaatgtaag ccagtctttg tgtcaagaag atgactatga agatgataag cctaccaatt
     4801 atagtgaacg ttactctgaa gaagaacagc atgaagaaga agagagacca acaaattata
     4861 gcataaaata taatgaagag aaacgtcatg tggatcagcc tattgattat agtttaaaat
     4921 atgccacaga tattccttca tcacagaaac agtcattttc attctcaaag agttcatctg
     4981 gacaaagcag taaaaccgaa catatgtctt caagcagtga gaatacgtcc acaccttcat
     5041 ctaatgccaa gaggcagaat cagctccatc caagttctgc acagagtaga agtggtcagc
     5101 ctcaaaaggc tgccacttgc aaagtttctt ctattaacca agaaacaata cagacttatt
     5161 gtgtagaaga tactccaata tgtttttcaa gatgtagttc attatcatct ttgtcatcag
     5221 ctgaagatga aataggatgt aatcagacga cacaggaagc agattctgct aataccctgc
     5281 aaatagcaga aataaaagaa aagattggaa ctaggtcagc tgaagatcct gtgagcgaag
     5341 ttccagcagt gtcacagcac cctagaacca aatccagcag actgcagggt tctagtttat
     5401 cttcagaatc agccaggcac aaagctgttg aattttcttc aggagcgaaa tctccctcca
     5461 aaagtggtgc tcagacaccc aaaagtccac ctgaacacta tgttcaggag accccactca
     5521 tgtttagcag atgtacttct gtcagttcac ttgatagttt tgagagtcgt tcgattgcca
     5581 gctccgttca gagtgaacca tgcagtggaa tggtaagtgg cattataagc cccagtgatc
     5641 ttccagatag ccctggacaa accatgccac caagcagaag taaaacacct ccaccacctc
     5701 ctcaaacagc tcaaaccaag cgagaagtac ctaaaaataa agcacctact gctgaaaaga
     5761 gagagagtgg acctaagcaa gctgcagtaa atgctgcagt tcagagggtc caggttcttc
     5821 cagatgctga tactttatta cattttgcca cagaaagtac tccagatgga ttttcttgtt
     5881 catccagcct gagtgctctg agcctcgatg agccatttat acagaaagat gtggaattaa
     5941 gaataatgcc tccagttcag gaaaatgaca atgggaatga aacagaatca gagcagccta
     6001 aagaatcaaa tgaaaaccaa gagaaagagg cagaaaaaac tattgattct gaaaaggacc
     6061 tattagatga ttcagatgat gatgatattg aaatactaga agaatgtatt atttctgcca
     6121 tgccaacaaa gtcatcacgt aaagcaaaaa agccagccca gactgcttca aaattacctc
     6181 cacctgtggc aaggaaacca agtcagctgc ctgtgtacaa acttctacca tcacaaaaca
     6241 ggttgcaacc ccaaaagcat gttagtttta caccggggga tgatatgcca cgggtgtatt
     6301 gtgttgaagg gacacctata aacttttcca cagctacatc tctaagtgat ctaacaatcg
     6361 aatcccctcc aaatgagtta gctgctggag aaggagttag aggaggagca cagtcaggtg
     6421 aatttgaaaa acgagatacc attcctacag aaggcagaag tacagatgag gctcaaggag
     6481 gaaaaacctc atctgtaacc atacctgaat tggatgacaa taaagcagag gaaggtgata
     6541 ttcttgcaga atgcattaat tctgctatgc ccaaagggaa aagtcacaag cctttccgtg
     6601 tgaaaaagat aatggaccag gtccagcaag catctgcgtc gtcttctgca cccaacaaaa
     6661 atcagttaga tggtaagaaa aagaaaccaa cttcaccagt aaaacctata ccacaaaata
     6721 ctgaatatag gacacgtgta agaaaaaatg cagactcaaa aaataattta aatgctgaga
     6781 gagttttctc agacaacaaa gattcaaaga aacagaattt gaaaaataat tccaaggact
     6841 tcaatgataa gctcccaaat aatgaagata gagtcagagg aagttttgct tttgattcac
     6901 ctcatcatta cacgcctatt gaaggaactc cttactgttt ttcacgaaat gattctttga
     6961 gttctctaga ttttgatgat gatgatgttg acctttccag ggaaaaggct gaattaagaa
     7021 aggcaaaaga aaataaggaa tcagaggcta aagttaccag ccacacagaa ctaacctcca
     7081 accaacaatc agctaataag acacaagcta ttgcaaagca gccaataaat cgaggtcagc
     7141 ctaaacccat acttcagaaa caatccactt ttccccagtc atccaaagac ataccagaca
     7201 gaggggcagc aactgatgaa aagttacaga attttgctat tgaaaatact ccagtttgct
     7261 tttctcataa ttcctctctg agttctctca gtgacattga ccaagaaaac aacaataaag
     7321 aaaatgaacc tatcaaagag actgagcccc ctgactcaca gggagaacca agtaaacctc
     7381 aagcatcagg ctatgctcct aaatcatttc atgttgaaga taccccagtt tgtttctcaa
     7441 gaaacagttc tctcagttct cttagtattg actctgaaga tgacctgttg caggaatgta
     7501 taagctccgc aatgccaaaa aagaaaaagc cttcaagact caagggtgat aatgaaaaac
     7561 atagtcccag aaatatgggt ggcatattag gtgaagatct gacacttgat ttgaaagata
     7621 tacagagacc agattcagaa catggtctat cccctgattc agaaaatttt gattggaaag
     7681 ctattcagga aggtgcaaat tccatagtaa gtagtttaca tcaagctgct gctgctgcat
     7741 gtttatctag acaagcttcg tctgattcag attccatcct ttccctgaaa tcaggaatct
     7801 ctctgggatc accatttcat cttacacctg atcaagaaga aaaacccttt acaagtaata
     7861 aaggcccacg aattctaaaa ccaggggaga aaagtacatt ggaaactaaa aagatagaat
     7921 ctgaaagtaa aggaatcaaa ggaggaaaaa aagtttataa aagtttgatt actggaaaag
     7981 ttcgatctaa ttcagaaatt tcaggccaaa tgaaacagcc ccttcaagca aacatgcctt
     8041 caatctctcg aggcaggaca atgattcata ttccaggagt tcgaaatagc tcctcaagta
     8101 caagtcctgt ttctaaaaaa ggcccacccc ttaagactcc agcctccaaa agccctagtg
     8161 aaggtcaaac agccaccact tctcctagag gagccaagcc atctgtgaaa tcagaattaa
     8221 gccctgttgc caggcagaca tcccaaatag gtgggtcaag taaagcacct tctagatcag
     8281 gatctagaga ttcgacccct tcaagacctg cccagcaacc attaagtaga cctatacagt
     8341 ctcctggccg aaactcaatt tcccctggta gaaatggaat aagtcctcct aacaaattat
     8401 ctcaacttcc aaggacatca tcccctagta ctgcttcaac taagtcctca ggttctggaa
     8461 aaatgtcata tacatctcca ggtagacaga tgagccaaca gaaccttacc aaacaaacag
     8521 gtttatccaa gaatgccagt agtattccaa gaagtgagtc tgcctccaaa ggactaaatc
     8581 agatgaataa tggtaatgga gccaataaaa aggtagaact ttctagaatg tcttcaacta
     8641 aatcaagtgg aagtgaatct gatagatcag aaagacctgt attagtacgc cagtcaactt
     8701 tcatcaaaga agctccaagc ccaaccttaa gaagaaaatt ggaggaatct gcttcatttg
     8761 aatctctttc tccatcatct agaccagctt ctcccactag gtcccaggca caaactccag
     8821 ttttaagtcc ttcccttcct gatatgtctc tatccacaca ttcgtctgtt caggctggtg
     8881 gatggcgaaa actcccacct aatctcagtc ccactataga gtataatgat ggaagaccag
     8941 caaagcgcca tgatattgca cggtctcatt ctgaaagtcc ttctagactt ccaatcaata
     9001 ggtcaggaac ctggaaacgt gagcacagca aacattcatc atcccttcct cgagtaagca
     9061 cttggagaag aactggaagt tcatcttcaa ttctttctgc ttcatcagaa tccagtgaaa
     9121 aagcaaaaag tgaggatgaa aaacatgtga actctatttc aggaaccaaa caaagtaaag
     9181 aaaaccaagt atccgcaaaa ggaacatgga gaaaaataaa agaaaatgaa ttttctccca
     9241 caaatagtac ttctcagacc gtttcctcag gtgctacaaa tggtgctgaa tcaaagactc
     9301 taatttatca aatggcacct gctgtttcta aaacagagga tgtttgggtg agaattgagg
     9361 actgtcccat taacaatcct agatctggaa gatctcccac aggtaatact cccccggtga
     9421 ttgacagtgt ttcagaaaag gcaaatccaa acattaaaga ttcaaaagat aatcaggcaa
     9481 aacaaaatgt gggtaatggc agtgttccca tgcgtaccgt gggtttggaa aatcgcctga
     9541 actcctttat tcaggtggat gcccctgacc aaaaaggaac tgagataaaa ccaggacaaa
     9601 ataatcctgt ccctgtatca gagactaatg aaagttctat agtggaacgt accccattca
     9661 gttctagcag ctcaagcaaa cacagttcac ctagtgggac tgttgctgcc agagtgactc
     9721 cttttaatta caacccaagc cctaggaaaa gcagcgcaga tagcacttca gctcggccat
     9781 ctcagatccc aactccagtg aataacaaca caaagaagcg agattccaaa actgacagca
     9841 cagaatccag tggaacccaa agtcctaagc gccattctgg gtcttacctt gtgacatctg
     9901 tttaaaagag aggaagaatg aaactaagaa aattctatgt taattacaac tgctatatag
     9961 acattttgtt tcaaatgaaa ctttaaaaga ctgaaaaatt ttgtaaatag gtttgattct
    10021 tgttagaggg tttttgttct cgaagccata tttgatagta tactttgtct tcactggtct
    10081 tattttggga ggcactcttg atggttagga aaaaaatagt aaagccaagt atgtttgtac
    10141 agtatgtttt acatgtattt aaagtagcat cccatcccaa cttcctttaa ttattgcttg
    10201 tcttaaaata atgaacacta cagatagaaa atatgatata ttgctgttat caatcatttc
    10261 tagattataa actgactaaa cttacatcag ggaaaaattg gtatttatgc aaaaaaaaat
    10321 gtttttgtgg aattcctgca gcccggggga tccgtcgagg aattcactcc tcaggtgcag
    10381 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
    10441 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
    10501 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
    10561 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
    10621 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
    10681 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
    10741 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
    10801 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
    10861 atagctgtcc ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat
    10921 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10981 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    11041 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    11101 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    11161 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    11221 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    12001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    12061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    12121 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    12181 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12241 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12301 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12361 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12421 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12481 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12541 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12601 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12661 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12721 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12781 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12841 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12901 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12961 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    13021 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    13081 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    13141 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    13201 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    13261 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    13321 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    13381 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    13441 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    13501 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttcacg ctgccgcaag
    13561 cactcagggc gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa
    13621 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
    13681 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
    13741 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
    13801 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
    13861 agatcctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
    13921 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
    13981 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt
    14041 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
    14101 aacagcgtgc cgcagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
    14161 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    14221 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    14281 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
    14341 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    14401 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    14461 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    14521 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    14581 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    14641 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
    14701 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    14761 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    14821 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    14881 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    14941 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    15001 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    15061 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg
    15121 agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
    15181 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
    15241 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
    15301 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
    15361 gcagccaacg tcggggcggc aagccctgcc atagccacgg gccccgtggg ttagggacgg
    15421 cggatcgcgg ccctctagag agcttggccc attgcatacg ttgtatccat atcataatat
    15481 gtacatttat attggctcat gtccaacatt accgccatgt t
//
