LOCUS Exported 9274 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION NMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC). ACCESSION . VERSION . KEYWORDS pKC-4.06 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9274) AUTHORS Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR TITLE ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. JOURNAL Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178. PUBMED 29528287 REFERENCE 2 (bases 1 to 9274) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9274 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 614..679 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 698..2344 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF VDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(761..779) /label=LucNrev /note="Firefly luciferase, reverse primer" protein_bind 2372..2396 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(3658..3682) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" polyA_signal 3716..4338 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3915..3934) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" primer_bind complement(4362..4382) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4380..4677 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind 4609..4628 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4731..5525 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4785..4804) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5395..5414 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6177..6311 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6214..6233) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6268..6287 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6316..6335) /label=T7 /note="T7 promoter, forward primer" promoter complement(6317..6335) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6349..6365) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6349..6365) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6362..6384) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6373..6389 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6397..6427) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6442..6463 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6580..6597) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6751..7339) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6831..6850) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7510..8370) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8133..8152 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8371..8475) /gene="bla" /label=AmpR promoter rep_origin 8502..8957 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8589..8608) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8799..8820 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 9083..9105 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 9097..9114 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 9098..9114 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agaattaacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta 661 caaggatgac gatgacaagc ttgcggccgc gaattcagaa gacgccaaaa acataaagaa 721 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc 781 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt 841 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg 901 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt 961 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta 1021 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc 1081 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat 1141 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac 1201 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga 1261 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc 1321 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa 1381 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat 1441 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga 1501 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc 1561 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt 1621 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa 1681 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat 1741 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt 1801 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga 1861 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa 1921 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga 1981 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt 2041 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt 2101 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca 2161 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc 2221 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa 2281 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc 2341 cgtggcgaat tcatcgatag atctgatatc aacaagtttg tacaaaaaag caggctccgc 2401 ggccgccccc ttcaccgtgc atctgactcc tgaggagaag tctgccgtta ctgccctgtg 2461 gggcaaggtg aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt 2521 acaagacagg tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg 2581 ggtttctgat aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc 2641 tggtggtcta cccttggacc tagaggttct ttgagtcctt tggggatctg tccactcctg 2701 atgctgttat gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta 2761 gtgatggcct ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc 2821 actgtgacaa gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct 2881 gggtttcctt cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat 2941 tctagggagc agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt 3001 ctctactcag aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt 3061 ggtaagcttt taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc 3121 catcctgata tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct 3181 ttctctaatc ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt 3241 ctttttaaat attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt 3301 ctcatagctc tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa 3361 tatattttac atataaattc tgtttgacat aggattctta taataatttg tcagtagttt 3421 aaggttgcaa acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca 3481 gttatgttga tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc 3541 tgggccacca cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg 3601 tggctggagt ggccactgcc ctggcccaca agtatcacta aaagggtggg cgcgccgacc 3661 cagctttctt gtacaaagtg gttgatatcg gtaccagtcg actctagagg atcccgggtg 3721 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc 3781 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta 3841 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg 3901 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac 3961 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg 4021 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt 4081 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc 4141 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt 4201 aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca 4261 accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca 4321 gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg 4381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 4441 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 4501 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 4681 aattgatcag cttgggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 5521 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 5581 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 5641 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg 5701 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt 5761 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca 5821 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac 5881 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt 5941 tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg 6001 ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg cgttgctggc gtttttccat 6061 aggctccgcc gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 6121 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 6301 atgtctggat caattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt 6361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 6781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 6841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 6901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 6961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 7201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 7501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 7561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 7621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 7681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 7741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 7801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 7861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 7921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 8041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 8101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 8161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 8221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 8281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 8341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 8401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 8461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc 8521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 8581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 8641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 8701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 8761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 8821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 8881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 8941 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg 9001 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta 9061 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag 9121 ctgatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca 9181 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt 9241 tatattggct catgtccaat atgaccgcca tgtt //