LOCUS       Exported                6111 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Express NIR-GECO1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hSyn-NES-NIR-GECO1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Qian Y, Piatkevich KD, Mc Larney B, Abdelfattah AS, Mehta S, Murdock
            MH, Gottschalk S, Molina RS, Zhang W, Chen Y, Wu J, Drobizhev M, 
            Hughes TE, Zhang J, Schreiter ER, Shoham S, Razansky D, Boyden ES, 
            Campbell RE
  TITLE     A genetically encoded near-infrared fluorescent calcium ion 
            indicator.
  JOURNAL   Nat Methods. 2019 Feb;16(2):171-174. doi: 10.1038/s41592-018-0294-6.
            Epub 2019 Jan 21.
  PUBMED    30664778
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     95..112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     promoter        592..1038
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    2621..3209
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2674..2694)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3092..3103)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3212..3228)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3213..3229)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3258..3379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3269..3288)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3323..3342
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3541..3670
                     /label=AAV2 ITR
     primer_bind     complement(3689..3706)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3689..3705)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3698..3720)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3847..4302
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3934..3953)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4144..4165
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4328..4432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4433..5293
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4651..4670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5464..6052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5953..5972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagaggg ccctgcgtat gagtgcaagt
      601 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccaccca
      661 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
      721 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
      781 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
      841 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
      901 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
      961 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
     1021 tcgtgcctga gagcgcagtc gaattcgggg atccatgctg cagaacgagc ttgctcttaa
     1081 gttggctgga cttgatatta acaagactgg aggaggttct atgtcggtac cgctgactac
     1141 ctcagcattc ggccacgcgt ttctggctaa ctgtgaacgt gagcagatcc acctggcggg
     1201 ctccattcag ccgcacggta tcctgctggc tgttaaagag cctgacaacg tggtgatcca
     1261 ggcttctatt aacgctgcgg agttcctgaa caccaacttt gttgttggcc gtccgctgcg
     1321 tgacctgggc ggcgatctgc ctttgcagat cctgccgcac ctgaacggcc cgctgcacct
     1381 ggctccgatg accctgcgtt gtactgtggg ttctccgccg cgtcgtgtgg actgtaccat
     1441 tcatcgaccg tctaacggcg gcctgatcgt agaactggaa ccagcaacca aggccactaa
     1501 cattgcgccg gctctggtcg gtgcgcttca tcgtatcact tcttcatcct ccctgatggg
     1561 cctgtgtgac gaaaccgcga ctattttccg tgagattact ggcttcgacc gtgtgatggt
     1621 aatgcgtctc ggcgcgcttg acgatctgac tgaagagcag atcgcagaga ttaaagaggc
     1681 tttctcccta tttgacaagg acggggacgg gacgataaca accaaggagc tggggacggt
     1741 gttccggtct ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt
     1801 agatgccgat ggcgacggca cattcgactt ccctgagttc ctgacgatga tggcaaggaa
     1861 aatgaatgac tcagacagtg aagaggaaat tagagaagcg ttccgcgtgt ttgataagga
     1921 cggcaatggc tacatcggcg cagcagagct tcgccacgtg atgacagacc ttggtgagaa
     1981 gttaactgat gaggaggttg atgaaatgat cagggtagca gacaacgatg gggatggtca
     2041 ggtaaactac gaagagtttg tacaaatgat gacagcgaag ggtggcggag gttctgtaga
     2101 ttcatcacgt cgtaagtgga ataaggcagg tcacgcagtc agagctatag gtcggctgag
     2161 ctcgcgtagg catgatttgc tgtccgaatg tcgtcgtgcg gacctggaag cgttcctggg
     2221 taaccgctac ccggcgtcta ctattccgca gatcgctcgt cgcctgtacg aacttaaccg
     2281 tgttcgcctg ctggtagatg tgaactatac tccggttccg ctacagccgc gcatcagccc
     2341 gctgaacggt cgtgatctgg atatgtccct gtcttgcctg cgctctatgt ccccgatcca
     2401 ccagaaatac atgcaggaca tgggcgttgg cgcaaccctg gtttgctctc tgatggtgtc
     2461 tggtcgtctg tggggtctga tcgtttgcca ccactacgaa ccgcgcttcg ttccgtccca
     2521 cattcgcgct gctggcgaag cgctggcgga aacttgtgcg aaccgcatcg cgacgctgga
     2581 gagctttgca cagtctcagt ccaaatgaaa gcttatcgat aatcaacctc tggattacaa
     2641 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2701 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2761 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2821 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2881 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2941 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3001 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3061 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3121 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3181 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac ccgggcggcc
     3241 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3301 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3361 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3421 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     3481 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
     3541 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     3601 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     3661 gagcgcgcag ccttaattaa cctaattcac tggccgtcgt tttacaacgt cgtgactggg
     3721 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3781 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     3841 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3901 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3961 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4021 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4081 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4141 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4201 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4261 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     4321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca t
//
