LOCUS Exported 6366 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter. ACCESSION . VERSION . KEYWORDS aav-SYN-tdNfsB(F124W)-mCherry (eNTR) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6366) AUTHORS Gruber TD, Krishnamurthy C, Grimm JB, Tadross MR, Wysocki LM, Gartner ZJ, Lavis LD TITLE Cell-Specific Chemical Delivery Using a Selective Nitroreductase-Nitroaryl Pair. JOURNAL ACS Chem Biol. 2018 Aug 29. doi: 10.1021/acschembio.8b00524. PUBMED 30111097 REFERENCE 2 (bases 1 to 6366) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6366 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 56..185 /label=AAV2 ITR promoter 267..714 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" CDS 2124..2831 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(2269..2287) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 2537..2556 /label=mCherry-F /note="mCherry, forward primer" misc_feature 2859..3447 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2912..2932) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3330..3341) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(3450..3466) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3451..3467) /label=pBluescriptKS /note="For pBluescript vector" polyA_signal 3496..3617 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3507..3526) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3561..3580 /label=SV40pA-R /note="SV40 polyA, reverse primer" repeat_region 3779..3908 /label=AAV2 ITR rep_origin 3980..4435 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4067..4086) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4277..4298 /label=F1ori-F /note="F1 origin, forward primer" promoter 4461..4565 /gene="bla" /label=AmpR promoter CDS 4566..5426 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4784..4803) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5597..6185 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6086..6105 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6339..6356 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg 61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg 121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg 181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa 241 gatctctgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag gatgaggcgg 301 ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc aacccccatt 361 ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg 421 tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc 481 caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc 541 cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg 601 aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg 661 ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagtcgaat 721 tctgaactag tgcaggtatg gatatcatct cggtggcatt gaaacgtcat tctaccaaag 781 ccttcgatgc gtcgaaaaag cttacgccag agcaagctga acagatcaag acgttgcttc 841 agtattcacc cagttcaact aattcccaac cctggcactt catcgtggcg tccacggagg 901 aagggaaagc gcgagtagcg aaatcagcag ccggcaacta tgtctttaac gaacgcaaaa 961 tgctcgatgc aagccacgtg gtggtctttt gtgccaaaac cgcaatggac gatgtatggc 1021 ttaagctggt agtggaccaa gaggatgcgg atggaaggtt cgctacaccg gaggcgaaag 1081 ccgcgaatga taagggaaga aagttctggg cagacatgca tcgaaaagac ctccatgatg 1141 acgcggagtg gatggccaag caggtttacc ttaatgtagg taacttcctg ttgggggtcg 1201 ccgcgttggg gctggacgct gtgcctattg aaggttttga cgccgccatt ttggatgcag 1261 agtttggact caaagaaaaa ggctacacta gcctcgtggt ggttccggtc ggtcaccact 1321 cggtggagga ctttaacgcg accctcccca agtcgcggct gccgcagaat atcacactca 1381 cggaagtggc tggttctagt gcaggtcaag gtgcacaggc tggtcaagga gcacaggctg 1441 gttctagtgc aggtatggat atcatctcgg tggcattgaa acgtcattct accaaagcct 1501 tcgatgcgtc gaaaaagctt acgccagagc aagctgaaca gatcaagacg ttgcttcagt 1561 attcacccag ttcaactaat tcccaaccct ggcacttcat cgtggcgtcc acggaggaag 1621 ggaaagcgcg agtagcgaaa tcagcagccg gcaactatgt ctttaacgaa cgcaaaatgc 1681 tcgatgcaag ccacgtggtg gtcttttgtg ccaaaaccgc aatggacgat gtatggctta 1741 agctggtagt ggaccaagag gatgcggatg gaaggttcgc tacaccggag gcgaaagccg 1801 cgaatgataa gggaagaaag ttctgggcag acatgcatcg aaaagacctc catgatgacg 1861 cggagtggat ggccaagcag gtttacctta atgtaggtaa cttcctgttg ggggtcgccg 1921 cgttggggct ggacgctgtg cctattgaag gttttgacgc cgccattttg gatgcagagt 1981 ttggactcaa agaaaaaggc tacactagcc tcgtggtggt tccggtcggt caccactcgg 2041 tggaggactt taacgcgacc ctccccaagt cgcggctgcc gcagaatatc acactcacgg 2101 aagtggctgg ttctagtgca ggtatggtga gcaagggcga ggaggataac atggccatca 2161 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 2221 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 2281 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 2341 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 2401 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 2461 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 2521 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 2581 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 2641 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 2701 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 2761 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 2821 agctgtacaa ggctggttct agatagaagc ttatcgataa tcaacctctg gattacaaaa 2881 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 2941 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 3001 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 3061 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 3121 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 3181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 3241 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 3301 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 3361 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 3421 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaccc gggcggccgc 3481 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 3541 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 3601 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 3661 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg 3721 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag 3781 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc 3841 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga 3901 gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 3961 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 4021 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 4081 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 4141 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 4201 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 4261 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 4321 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 4381 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg 4441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 4501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 4561 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 4621 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 4681 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 4741 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 4801 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4861 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4921 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4981 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 5041 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 5101 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 5161 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 5221 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 5281 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 5341 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 5401 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 5461 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 5521 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 5581 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 5641 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 5701 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 5761 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 5821 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5881 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5941 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 6001 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 6061 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 6121 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 6181 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 6241 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 6301 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 6361 cggaag //