LOCUS Exported 8920 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral expression vector for direct cardiac reprogramming. ACCESSION . VERSION . KEYWORDS pMx-MGT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8920) AUTHORS Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian L TITLE Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming. JOURNAL Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21. PUBMED 25416133 REFERENCE 2 (bases 1 to 8920) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8920 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1094..1510 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1440..1462 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1478..1494 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1520..1894 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1832..1848 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 3237..3293 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" CDS 4638..4691 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" LTR 6425..6894 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(7024..7041) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7195..7783) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7275..7294) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7947..8807) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8570..8589 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8808..8912) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 ccgccatggg gagaaaaaag attcagatta cgaggataat ggatgagcgt aacagacagg 1981 tgacttttac gaagaggaaa tttggattga tgaagaaggc ttatgagctg agcgtgctgt 2041 gcgactgtga gattgcactg atcatcttca acagcaccaa caagctgttc cagtacgcca 2101 gcactgacat ggataaggtg ttgctcaagt acaccgagta caacgagccg cacgagagcc 2161 ggacaaactc agacattgtg gaggcattga acaagaaaga aaacaaaggc tctgaaagcc 2221 ccgatcctga ctcctcttat gcactcaccc cacgcactga agaaaaatac aaaaaaatta 2281 atgaagaatt tgataatatg atcaagagtc ataaaattcc tgctgttcca cctcccagct 2341 ttgagatgcc agttaccatc ccagtgtcca gccataacag tttggtgtac agcaatcctg 2401 tcagcacact gggaaacccc aatcttctgc cactggccca cccgtctctg cagaggaata 2461 gtatgtctcc tggtgtaaca catagacctc caagtgcagg taacacaggc ggtctgatgg 2521 gcggagatct gacatccggt gcaggcacca gcgcagggaa tggatacggc aacccccgga 2581 actcaccagg cctgctggtc tcacctggta acctgaacaa gaatatacaa gccaaatctc 2641 ctccccctat gaatctagga atgaataatc gtaagccaga tctccgcgtt cttatcccac 2701 ctggcagcaa gaacacgatg ccatcagtga atcaaaggat aaataactcc cagtcggctc 2761 agtcattggc taccccggtg gtttccgtag caactcctac tttaccagga caaggaatgg 2821 gaggatatcc atcagccatt tcaacaacat atggtactga gtactctctg agtagcgcag 2881 atctgtcatc tctgtctggc ttcaacactg ccagtgcgct ccacctcggc tctgtaactg 2941 gctggcagca gcagcaccta cataacatgc cgccatctgc cctcagtcag ttgggagacc 3001 gtaccaccac cccttcgaga tacccacaac acaccacgcg ccacgaggcg gggaggtctc 3061 ctgttgacag cttgagcagc tgtagcagtt cctacgatgg gagcgaccga gaggatcacc 3121 ggaacgaatt ccactccccc attggactca ccagaccttc gccggacgaa agggaaagtc 3181 cttcagtcaa gcgcatgcga ctctctgaag gatgggcaac agctagcggc agcggcgcca 3241 cgaacttctc tctgttaaag caagcaggag atgttgaaga aaaccccggg cctgcatgca 3301 tgtaccaaag cctggccatg gccgccaacc acggcccccc gcccggcgcc tacgaagcag 3361 gtggccctgg cgccttcatg cacagcgcgg gcgccgcgtc ctcgcccgtc tacgtgccca 3421 ctccgcgggt gccgtcctct gtgctgggcc tgtcctacct gcagggcggt ggcagtgccg 3481 ctgcagctgg aaccacctcg ggtggcagct ccggggccgg cccgtcgggt gcagggcctg 3541 ggacccagca gggtagccct ggctggagcc aagctggagc cgagggagcc gcctacaccc 3601 cgccgcccgt gtccccgcgc ttctctttcc cggggactac tgggtccctg gcggccgctg 3661 ccgccgctgc cgcagcccgg gaagctgcag cctacggcag tggcggcggg gcggcgggcg 3721 ctggtctggc tggccgagag cagtacgggc gtccgggctt cgccggctcc tactccagcc 3781 cctacccagc ctacatggcc gacgtgggag catcctgggc cgcagccgct gccgcctctg 3841 ccggcccctt cgacagccca gtcctgcaca gcctgcctgg acgggccaac cctggaagac 3901 accccaatct cgatatgttt gatgacttct cagaaggcag agagtgtgtc aattgtgggg 3961 ccatgtccac cccactctgg aggcgagatg ggacgggaca ctacctgtgc aatgcctgtg 4021 gcctctatca caagatgaac ggcatcaacc ggcccctcat taagcctcag cgccgcctgt 4081 ccgcttcccg ccgggtaggc ctctcctgtg ccaactgcca gactaccacc accacgctgt 4141 ggcgtcgtaa tgccgagggt gagcctgtat gtaatgcctg cggcctctac atgaagctcc 4201 atggggttcc caggcctctt gcaatgcgga aggaggggat tcaaaccaga aaacggaagc 4261 ccaagaacct gaataaatct aagacgccag caggtcctgc tggtgagacc ctccctccct 4321 ccagtggtgc ctccagcggt aactccagca atgccactag cagcagcagc agcagtgaag 4381 agatgcgccc catcaagaca gagcccgggc tgtcatctca ctatgggcac agcagctcca 4441 tgtcccagac attcagtact gtgtccggcc acgggccctc catccatcca gtgctgtctg 4501 ctctgaagct gtccccacaa ggctatgcat ctcctgtcac tcagacatcg caggccagct 4561 ccaagcagga ctcttggaac agcctggtcc tggctgacag tcatggggac ataatcaccg 4621 cggcatgcgg ctccggcgag ggcaggggaa gtcttctaac atgcggggac gtggaggaaa 4681 atcccggccc actcgagatg gccgatacag atgagggctt tggcctggcg cgcacgcctc 4741 tggagcctga ttccaaagac aggtcttgcg attcgaaacc tgagagtgct ctgggggctc 4801 ccagcaagtc tccatcatcc ccgcaggctg ccttcaccca gcagggcatg gaaggaatca 4861 aggtgtttct tcatgaacgt gaactgtggc tgaagttcca cgaagtgggc acagagatga 4921 tcatcaccaa ggcagggagg agaatgtttc ctagttacaa agtgaaggtg actggcctta 4981 atcccaaaac gaagtatatt cttctcatgg atattgttcc cgcagacgac cacagatata 5041 aatttgctga taacaaatgg tccgtaactg gcaaagcaga gcctgccatg ccggggcgcc 5101 tttacgtgca cccggactcc ccagcaaccg gagcccactg gatgcgacaa cttgtctcct 5161 tccagaagct caaactcacc aacaaccacc tggacccgtt tggacacatt atcctgaact 5221 ccatgcacaa ataccagccc cgattacaca tcgtgaaagc agacgaaaat aatgggttcg 5281 gttcaaagaa cactgcgttt tgcacccacg tcttcccgga gacagctttt atcgctgtga 5341 cttcgtacca gaatcacaag atcacacagc tgaaaattga gaacaacccc ttcgccaaag 5401 gctttcgggg cagtgatgac ctggagttac acaggatgtc tcggatgcaa agtaaagagt 5461 atcctgtggt tcccaggagc acagtgaggc acaaagtcac ctccaaccac agccccttca 5521 gcagcgagac ccgagctctc tccacctcat ccaatttagg gtcccagtac cagtgtgaga 5581 atggtgtctc tggcccctcc caggaccttc tgcccccacc taacccatac ccactggccc 5641 aggagcacag ccaaatttac cactgtacca agaggaaaga tgaggaatgt tccagcacgg 5701 agcaccccta taagaagccg tacatggaga catcccccag cgaggaagac accttctatc 5761 gctcgggcta cccccagcag cagggcctga gtacctctta caggacagag tcggcccagc 5821 ggcaggcctg catgtatgcc agctccgctc cccccagcga gcccgtgcct agcctggagg 5881 acatcagctg taacacatgg cccagcatgc cctcctatag cagctgtacc gtcaccaccg 5941 tgcagcccat ggaccgtctt ccctaccagc acttctccgc tcatttcacc tcggggcccc 6001 tggtccctcg gttggctggc atggccaacc atggttctcc ccagctcggc gaagggatgt 6061 ttcagcacca gacctcagtg gcccatcagc ctgtggtcag gcagtgcggg cctcagactg 6121 gccttcagtc tccgggcggc ctccagcccc cagagtttct ctacactcac ggcgtgccca 6181 ggaccctgtc cccccatcag tatcactcgg tacacggcgt cggcatggtg ccagagtgga 6241 gtgagaatag ctaagtcgac gataaaataa aagattttat ttagtctcca gaaaaagggg 6301 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag 6361 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga 6421 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg 6481 ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc 6541 tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt 6601 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta 6661 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct 6721 caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc 6781 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt 6841 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca 6901 gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt 6961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7861 attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt 7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc //