LOCUS Exported 2248 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Promoter J23118 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::J23118 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2248) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2248) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2248 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..95 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 182..268 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(281..299) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 367..471 /gene="bla" /label=AmpR promoter CDS 472..1332 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(690..709) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1503..2091 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1992..2011 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc 61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gcagttgacg 121 gctagctcag tcctaggtat tgtgctagcc catgaagagc gtaagacctc tagggcggcg 181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt 241 ctttcgactg agcctttcgt tttatttgat gcccgaaagg gcctcgtgat acgcctattt 301 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 1021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 1081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 1141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc 1201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 1261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 1321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 1381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 1441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 1501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 1561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 1621 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 1681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 1741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 1801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 1861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 1921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 1981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 2041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 2101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 2161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 2221 cgccgcagcc gaacgaccga gcgccttg //