LOCUS Exported 2243 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION RBS RBSc44 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::RBSc44 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2243) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2243) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2243 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..95 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 177..263 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(276..294) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 362..466 /gene="bla" /label=AmpR promoter CDS 467..1327 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(685..704) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1498..2086 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1987..2006 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc 61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gccaatcgga 121 ttggatccaa aggaggttat accgatgtga agagcgtaag acctctaggg cggcggattt 181 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc 241 gactgagcct ttcgttttat ttgatgcccg aaagggcctc gtgatacgcc tatttttata 301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 1021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 1081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 1141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtcccgcg gtatcattgc 1201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 1261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 1321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 1381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 1441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 1501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 1561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 1621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 1681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 1741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 1801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 1861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 1921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 1981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 2041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 2101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 2161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 2221 cagccgaacg accgagcgcc ttg //