LOCUS Exported 2244 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION RBS RBSc58 in pStA0. ACCESSION . VERSION . KEYWORDS pStA0::RBSc58 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2244) AUTHORS Taylor GM, Mordaka PM, Heap JT TITLE Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering. JOURNAL Nucleic Acids Res. 2018 Nov 20. pii: 5193345. doi: 10.1093/nar/gky1182. PUBMED 30462270 REFERENCE 2 (bases 1 to 2244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2244 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 1..95 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 178..264 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(277..295) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 363..467 /gene="bla" /label=AmpR promoter CDS 468..1328 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(686..705) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1499..2087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1988..2007 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc 61 tcggttgccg ccgggcgttt tttattggtg agaatccagt gtggctcttc gccacatgat 121 cgaatgatta aaggaggttg gaggtatgtg aagagcgtaa gacctctagg gcggcggatt 181 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt 241 cgactgagcc tttcgtttta tttgatgccc gaaagggcct cgtgatacgc ctatttttat 301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtcccgc ggtatcattg 1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 1621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 1861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2221 gcagccgaac gaccgagcgc cttg //