LOCUS Exported 6252 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDONR223-JAK1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6252) AUTHORS Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA, Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM, Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA TITLE COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. JOURNAL Nature. 2010 Nov 24. ():. PUBMED 21107320 REFERENCE 2 (bases 1 to 6252) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6252 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 98..115 /label=L4440 /note="L4440 vector, forward primer" terminator 274..301 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 393..479 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 528..550 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 542..559 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 543..559 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 575..674 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(4253..4272) /label=T7 /note="T7 promoter, forward primer" promoter complement(4254..4272) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4277..4293) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4277..4293) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 4724..5515 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 5608..6196 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6097..6116 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg 241 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc 301 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt 361 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc 421 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga 481 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg 541 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat 601 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta 661 caaaaaagtt ggcatgcagt atctaaatat aaaagaggac tgcaatgcca tggctttctg 721 tgctaaaatg aggagctcca agaagactga ggtgaacctg gaggcccctg agccaggggt 781 ggaagtgatc ttctatctgt cggacaggga gcccctccgg ctgggcagtg gagagtacac 841 agcagaggaa ctgtgcatca gggctgcaca ggcatgccgt atctctcctc tttgtcacaa 901 cctctttgcc ctgtatgacg agaacaccaa gctctggtat gctccaaatc gcaccatcac 961 cgttgatgac aagatgtccc tccggctcca ctaccggatg aggttctatt tcaccaattg 1021 gcatggaacc aacgacaatg agcagtcagt gtggcgtcat tctccaaaga agcagaaaaa 1081 tggctacgag aaaaaaaaga ttccagatgc aacccctctc cttgatgcca gctcactgga 1141 gtatctgttt gctcagggac agtatgattt ggtgaaatgc ctggctccta ttcgagaccc 1201 caagaccgag caggatggac atgatattga gaacgagtgt ctagggatgg ctgtcctggc 1261 catctcacac tatgccatga tgaagaagat gcagttgcca gaactgccca aggacatcag 1321 ctacaagcga tatattccag aaacattgaa taagtccatc agacagagga accttctcac 1381 caggatgcgg ataaataatg ttttcaagga tttcctaaag gaatttaaca acaagaccat 1441 ttgtgacagc agcgtgtcca cgcatgacct gaaggtgaaa tacttggcta ccttggaaac 1501 tttgacaaaa cattacggtg ctgaaatatt tgagacttcc atgttactga tttcatcaga 1561 aaatgagatg aattggtttc attcgaatga cggtggaaac gttctctact acgaagtgat 1621 ggtgactggg aatcttggaa tccagtggag gcataaacca aatgttgttt ctgttgaaaa 1681 ggaaaaaaat aaactgaagc ggaaaaaact ggaaaataaa cacaagaagg atgaggagaa 1741 aaacaagatc cgggaagagt ggaacaattt ttcttacttc cctgaaatca ctcacattgt 1801 aataaaggag tctgtggtca gcattaacaa gcaggacaac aagaaaatgg aactgaagct 1861 ctcttcccac gaggaggcct tgtcctttgt gtccctggta gatggctact tccggctcac 1921 agcagatgcc catcattacc tctgcaccga cgtggccccc ccgttgatcg tccacaacat 1981 acagaatggc tgtcatggtc caatctgtac agaatacgcc atcaataaat tgcggcaaga 2041 aggaagcgag gaggggatgt acgtgctgag gtggagctgc accgactttg acaacatcct 2101 catgaccgtc acctgctttg agaagtctga gcaggtgcag ggtgcccaga agcagttcaa 2161 gaactttcag atcgaggtgc agaagggccg ctacagtctg cacggttcgg accgcagctt 2221 ccccagcttg ggagacctca tgagccacct caagaagcag atcctgcgca cggataacat 2281 cagcttcatg ctaaaacgct gctgccagcc caagccccga gaaatctcca acctgctggt 2341 ggctactaag aaagcccagg agtggcagcc cgtctacccc atgagccagc tgagtttcga 2401 tcggatcctc aagaaggatc tggtgcaggg cgagcacctt gggagaggca cgagaacaca 2461 catctattct gggaccctga tggattacaa ggatgacgaa ggaacttctg aagagaagaa 2521 gataaaagtg atcctcaaag tcttagaccc cagccacagg gatatttccc tggccttctt 2581 cgaggcagcc agcatgatga gacaggtctc ccacaaacac atcgtgtacc tctatggcgt 2641 ctgtgtccac gacgtggaga atatcatggt ggaagagttt gtggaagggg gtcctctgga 2701 tctcttcatg caccggaaaa gcgatgtcct taccacacca tggaaattca aagttgccaa 2761 acagctggcc agtgccctga gctacttgga ggataaagac ctggtccatg gaaatgtgtg 2821 tactaaaaac ctcctcctgg cccgtgaggg catcgacagt gagtgtggcc cattcatcaa 2881 gctcagtgac cccggcatcc ccattacggt gctgtctagg caagaatgca ttgaacgaat 2941 cccatggatt gctcctgagt gtgttgagga ctccaagaac ctgagtgtgg ctgctgacaa 3001 gtggagcttt ggaaccacgc tctgggaaat ctgctacaat ggcgagatcc ccttgaaaga 3061 caagacgctg attgagaaag agagattcta tgaaagccgg tgcaggccag tgacaccatc 3121 atgtaaggag ctggctgacc tcatgacccg ctgcatgaac tatgacccca atcagaggcc 3181 tttcttccga gccatcatga gagacattaa taagcttgaa gagcagaatc cagatattgt 3241 ttcagaaaaa aaaccagcaa ctgaagtgga ccccacacat tttgaaaagc gcttcctaaa 3301 gaggatccgt gacttgggag agggccactt tgggaaggtt gagctctgca ggtatgaccc 3361 cgaaggggac aatacagggg agcaggtggc tgttaaatct ctgaagcctg agagtggagg 3421 taaccacata gctgatctga aaaaggaaat cgagatctta aggaacctct atcatgagaa 3481 cattgtgaag tacaaaggaa tctgcacaga agacggagga aatggtatta agctcatcat 3541 ggaatttctg ccttcgggaa gccttaagga atatcttcca aagaataaga acaaaataaa 3601 cctcaaacag cagctaaaat atgccgttca gatttgtaag gggatggact atttgggttc 3661 tcggcaatac gttcaccggg acttggcagc aagaaatgtc cttgttgaga gtgaacacca 3721 agtgaaaatt ggagacttcg gtttaaccaa agcaattgaa accgataagg agtattacac 3781 cgtcaaggat gaccgggaca gccctgtgtt ttggtatgct ccagaatgtt taatgcaatc 3841 taaattttat attgcctctg acgtctggtc ttttggagtc actctgcatg agctgctgac 3901 ttactgtgat tcagattcta gtcccatggc tttgttcctg aaaatgatag gcccaaccca 3961 tggccagatg acagtcacaa gacttgtgaa tacgttaaaa gaaggaaaac gcctgccgtg 4021 cccacctaac tgtccagatg aggtttatca acttatgagg aaatgctggg aattccaacc 4081 atccaatcgg acaagctttc agaaccttat tgaaggattt gaagcacttt taaaattgcc 4141 aactttcttg tccaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga 4201 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag 4261 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct 4321 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga 4381 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg 4441 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta 4501 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc 4561 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 4621 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 4681 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat 4741 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc 4801 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag 4861 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt 4921 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt 4981 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga 5041 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac 5101 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt 5161 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct 5221 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt 5281 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga 5341 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga 5401 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt 5461 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc 5521 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 5581 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 5641 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 5701 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 5761 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 5821 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 5881 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 5941 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6001 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 6061 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 6121 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 6181 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 6241 gccttttgct ca //