LOCUS       Exported               11389 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  lentiviral expression of SV40 large T antigen and mCherry red 
            fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pRRLsin-SV40 T antigen-IRES-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, 
            Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
  TITLE     Modeling pathogenesis of primary liver cancer in lineage-specific 
            mouse cell types.
  JOURNAL   Gastroenterology. 2013 Jul;145(1):221-31. doi: 
            10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
  PUBMED    23523670
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1085..1318
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1503..1547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1814..1931
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1953..2332
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2335..2610
                     /label=chicken beta-actin promoter
     intron          2611..3619
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3543..3565
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3627..3646
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3718..5844
                     /codon_start=1
                     /product="large T antigen from SV40 (simian virus 40)"
                     /label=large T antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
                     CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
                     TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
                     CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
                     CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
                     VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
                     KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
                     FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
                     TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
                     LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
                     DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
                     CFKKPPTPPPEPET"
     misc_feature    5913..6499
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6080..6097)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6307..6326
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6500..7210
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6645..6663)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6913..6932
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7230..7246)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7230..7246)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    7261..7849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7314..7334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7732..7743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7936..8169
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8241..8362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8278..8297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8332..8351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8381..8516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8443..8462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8536..8555)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8537..8555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8565..8582)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8565..8581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8574..8596)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8723..9178
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8810..8829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9020..9041
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9204..9308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9309..10169
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9527..9546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10340..10928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10829..10848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11082..11099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11216..11237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11252..11282
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11290..11306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11295..11317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11314..11330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11314..11330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11349..11369
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11351..11369
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggcct ggccgctgat cttcagacct ggaggaggag
      961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     1021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     1081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     1141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     1201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     1261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     1321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     1381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     1441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     1501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     1561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     1621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     1681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     1741 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
     1801 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1861 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     1921 ttcaaaattt tatcgatcac gagactagcc tcgacattga ttattgacta gttattaata
     1981 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2041 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2101 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta
     2161 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2221 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2281 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg
     2341 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
     2401 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg
     2461 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
     2521 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
     2581 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc
     2641 ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     2701 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     2761 ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc
     2821 gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     2881 cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2941 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg
     3001 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc
     3061 ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     3121 cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     3181 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     3241 ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     3301 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg
     3361 aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg
     3421 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     3481 atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc
     3541 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     3601 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     3661 tggcaaagaa ttcctcgagg atgaggatcc cgcgaccggt cgaccagctt tgcaaagatg
     3721 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     3781 agtgcctggg ggaatattcc tctgatgaga aaggcatatt taaaaaaatg caaggagttt
     3841 catcctgata aaggaggaga tgaagaaaaa atgaagaaaa tgaatactct gtacaagaaa
     3901 atggaagatg gagtaaaata tgctcatcaa cctgactttg gaggcttctg ggatgcaact
     3961 gagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     4021 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     4081 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     4141 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     4201 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     4261 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     4321 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     4381 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatccattt
     4441 tctgttattg aggaaagttt gccaggtggg ttaaaggagc atgattttaa tccagaagaa
     4501 gcagaggaaa ctaaacaagt gtcctggaag cttgtaacag agtatgcaat ggaaacaaaa
     4561 tgtgatgatg tgttgttatt gcttgggatg tacttggaat ttcagtacag ttttgaaatg
     4621 tgtttaaaat gtattaaaaa agaacagccc agccactata agtaccatga aaagcattat
     4681 gcaaatgctg ctatatttgc tgacagcaaa aaccaaaaaa ccatatgcca acaggctgtt
     4741 gatactgttt tagctaaaaa gcgggttgat agcctacaat taactagaga acaaatgtta
     4801 acaaacagat ttaatgatct tttggatagg atggatataa tgtttggttc tacaggctct
     4861 gctgacatag aagaatggat ggctggagtt gcttggctac actgtttgtt gcccaaaatg
     4921 gattcagtgg tgtatgactt tttaaaatgc atggtgtaca acattcctaa aaaaagatac
     4981 tggctgttta aaggaccaat tgatagtggt aaaactacat tagcagctgc tttgcttgaa
     5041 ttatgtgggg ggaaagcttt aaatgttaat ttgcccttgg acaggctgaa ctttgagcta
     5101 ggagtagcta ttgaccagtt tttagtagtt tttgaggatg taaagggcac tggaggggag
     5161 tccagagatt tgccttcagg tcagggaatt aataacctgg acaatttaag ggattatttg
     5221 gatggcagtg ttaaggtaaa cttagaaaag aaacacctaa ataaaagaac tcaaatattt
     5281 ccccctggaa tagtcaccat gaatgagtac agtgtgccta aaacactgca ggccagattt
     5341 gtaaaacaaa tagattttag gcccaaagat tatttaaagc attgcctgga acgcagtgag
     5401 tttttgttag aaaagagaat aattcaaagt ggcattgctt tgcttcttat gttaatttgg
     5461 tacagacctg tggctgagtt tgctcaaagt attcagagca gaattgtgga gtggaaagag
     5521 agattggaca aagagtttag tttgtcagtg tatcaaaaaa tgaagtttaa tgtggctatg
     5581 ggaattggag ttttagattg gctaagaaac agtgatgatg atgatgaaga cagccaggaa
     5641 aatgctgata aaaatgaaga tggtggggag aagaacatgg aagactcagg gcatgaaaca
     5701 ggcattgatt cacagtccca aggctcattt caggcccctc agtcctcaca gtctgttcat
     5761 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaacg gggatcctca
     5881 tcgcggccgc tacgtaaatt ccgccccccc cccccctctc cctccccccc ccctaacgtt
     5941 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     6001 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     6061 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6121 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6181 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6241 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6301 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6361 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     6421 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     6481 tgataatatg gccacaacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6541 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     6601 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     6661 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     6721 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     6781 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     6841 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     6901 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     6961 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     7021 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     7081 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     7141 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     7201 gtacaagtaa gaattcctgc agcccggggg atccactagt tctagagcgg ccgcgtcgac
     7261 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7321 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7381 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7441 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7501 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7561 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7621 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     7681 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7741 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7801 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     7861 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7921 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7981 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8041 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8101 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8161 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     8221 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     8281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8341 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     8401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8461 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8521 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     8581 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     8641 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     8701 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     8761 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     8821 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     8881 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     8941 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9001 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9061 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9121 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9181 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     9481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     9541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     9601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     9661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     9721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10021 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    10501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11101 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    11221 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    11281 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    11341 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgca
//
