LOCUS Exported 4741 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581. ACCESSION . VERSION . KEYWORDS mEos2-Farnesyl-5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4741) AUTHORS Jaqaman K, Galbraith JA, Davidson MW, Galbraith CG TITLE Changes in single-molecule integrin dynamics linked to local cellular behavior. JOURNAL Mol Biol Cell. 2016 May 15;27(10):1561-9. doi: 10.1091/mbc.E16-01-0018. Epub 2016 Mar 23. PUBMED 27009207 REFERENCE 2 (bases 1 to 4741) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4741 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 54..357 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 358..561 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 512..532 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 606..1283 /codon_start=1 /product="monomeric variant of green-to-red photoswitchable fluorescent protein EosFP (McKinney et al., 2009)" /label=mEos2 /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 1522..1643 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1559..1578) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1613..1632 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1650..2105) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1787..1808) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1999..2018 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2132..2236 /gene="bla" /label=AmpR promoter promoter 2238..2595 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2261..2281) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2446..2581 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2508..2527 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2630..3424 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2684..2703) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3294..3313 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3612..3631) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3656..3703 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4032..4620 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4521..4540 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg 601 ccaccatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa ggcaacgtaa 661 acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag ggaaaacaga 721 gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat atcctgacca 781 ctgcattcca ttacggcaac agggtattcg ccaaatatcc agacaacata caagactatt 841 ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc gaagacgggg 901 gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat aataaagttc 961 gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag acgctgaaat 1021 gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt gatattcata 1081 tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact acttacaaag 1141 ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc attgagattt 1201 taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt gctcattctg 1261 gattgcctga caatgccaga cgatccggac tcagatctaa gctgaaccct cctgatgaga 1321 gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg agaattctgc agtcgacggt 1381 accgcgggcc cgggatccac cggatctaga taactgatca taatcagcca taccacattt 1441 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 1501 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 1561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 1621 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt 1681 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 1741 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 1801 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 1861 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 1921 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 1981 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 2041 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 2101 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 2161 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 2221 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 2281 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 2341 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2401 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 2461 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 2521 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 2581 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 2641 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 2701 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 2761 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 2821 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 2881 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 2941 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3001 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 3061 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 3121 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 3181 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 3241 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 3301 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 3361 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 3421 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 3481 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 3541 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 3601 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 3661 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 3721 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 3781 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 3841 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 3901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 3961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 4201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4681 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta 4741 t //