LOCUS       Exported                 724 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    Flag-hPIASx beta
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Arora T, Liu B, He H, Kim J, Murphy TL, Murphy KM, Modlin RL, Shuai 
            K
  TITLE     PIASx is a transcriptional co-repressor of signal transducer and 
            activator of transcription 4.
  JOURNAL   J Biol Chem. 2003 Jun 13. 278(24):21327-30.
  PUBMED    12716907
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      27..36
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctcgttta gtgaaccgtc agaattgcca ccatggacta caaggacgac gatgacaaga
       61 attctgcagg atccaccatg gcggatttcg aagagttgag gaatatggtt tctagtttta
      121 gggtttctga actacaagta ttactaggct ttgctggacg gaataaaagt ggacgcaagc
      181 atgacctcct gatgagggcg ctgcatttat tgaagagcgg ctgcagccct gcggttcaga
      241 ttaaaatccg agaattgtat agacgccgat atccacgaac tcttgaagga ctttctgatt
      301 tatccacaat caaatcatcg gttttcagtt tggatggtgg ctcatcacct gtagaacctg
      361 acttggccgt ggctggaatc cactcgttgc cttccacttc agttacacct cactcaccat
      421 cctctcctgt tggttctgtg ctgcttcaag atactaagcc cacatttgag atgcagcagc
      481 catctccccc aattcctcct gtccatcctg atgtgcagtt aaaaaatctg cccttttatg
      541 atgtccttga tgttctcatc aagcccacga gtttagttca aagcagtatt cagcgatttc
      601 aagagaagtt ttttattttt gctttgacac ctcaacaagt tagagagata tgcatatcca
      661 gggatttttt gccaggtggt aggagagatt atacagtcca agttcagttg agactttgcc
      721 tggc
//
