LOCUS       Exported                9266 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 
            along with IRES mCherry as a fluorescent marker..
ACCESSION   .
VERSION     .
KEYWORDS    Gal4DB-NS3-VP64-p65_IRES_mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Tague EP, Dotson HL, Tunney SN, Sloas DC, Ngo JT
  TITLE     Chemogenetic control of gene expression and cell signaling with 
            antiviral drugs.
  JOURNAL   Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y.
            Epub 2018 Jul 2.
  PUBMED    29967495
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(476..1336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1099..1118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1337..1441)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1516..1535)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1707..2086
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2087..2290
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2241..2261
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2335..2354
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2335..2353
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3685..3711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3730..3750
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    3787..3811
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3841..3990
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4015..4035
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4465..4821
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5418..6128
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5563..5581)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5831..5850
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6160..6177)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6166..6390
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6436..6864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6523..6542)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6733..6754
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6873..6893)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6878..7207
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7058..7193
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7120..7139
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7256..8281
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8411..8532
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8448..8467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8502..8521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8581..8597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8581..8597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8594..8616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8605..8621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8629..8659)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8674..8695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8812..8829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8983..305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9063..9082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
       61 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      121 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      181 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      241 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      601 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
      661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
      721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      781 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      841 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      901 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      961 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1021 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1081 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     1141 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     1201 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     1261 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     1321 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1381 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1441 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     1501 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     1561 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1621 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1681 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     1741 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1801 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1861 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1921 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1981 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2041 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2101 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2161 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2221 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2281 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
     2341 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatgaag
     2401 ttgctgagca gcatagagca agcatgtgat atctgccggt tgaagaagct gaagtgtagc
     2461 aaggagaagc ccaagtgcgc caagtgtctc aagaataatt gggagtgtag gtatagcccc
     2521 aagaccaagc gaagcccgct tacgagagca caccttaccg aggtcgagag ccgcctggaa
     2581 agactcgaac aactttttct tctgattttc cccagggagg acctggacat gatcctgaag
     2641 atggacagcc tccaggacat caaagccctt cttaccgggc tgttcgtgca ggacaacgtc
     2701 aacaaggatg cggtgaccga cagattggcg agcgtggaga cggacatgcc cttgaccctc
     2761 agacaacata ggatcagcgc gacaagctca tctgaagaat ctagcaataa gggacagcga
     2821 cagctgaccg ttagtggggc gtctgcaggc atggccagca tgactggtgg acagcaaatg
     2881 gggtcgacgg aggacgtggt gtgctgccac tcaatctacg gcaagaagaa gggtgatatc
     2941 gacacctacc gatacatagg ctcttccggg acaggctgcg tggtcatagt gggcaggatc
     3001 gtcttgtccg gatccggcac tagtgcgccc atcacggcgt acgcccagca gacgagaggc
     3061 ctcctagggt gtataatcac cagcctgact ggccgggaca aaaaccaagt ggagggtgag
     3121 gtccagatcg tgtcaactgc tacccaaacc ttcctggcaa cgtgcatcaa tggggtatgc
     3181 tgggcagtct accacggggc cggaacgagg accatcgcat cacccaaggg tcctgtcatc
     3241 cagatgtata ccaatgtgga ccaagacctt gtgggctggc ccgctcctca aggttcccgc
     3301 tcattgacac cctgtacctg cggctcctcg gacctttacc tggtcacgag gcacgccgat
     3361 gtcattcccg tgcgccggcg aggtgatagc aggggtagcc tgctttcgcc ccggcccatt
     3421 tcctacttga aaggctcctc ggggggtccg ctgttgtgcc ccgcgggaca cgccgtgggc
     3481 ctattcaggg ccgcggtgtg cacccgtgga gtggctaaag cggtggactt tatccctgtg
     3541 gagaacctag agacaaccat gagatccccg gtgttcacgg acaactcctc tccaccagca
     3601 gtcaccctga cgcacccaat caccaaaatc gatagggagg ttctctacca ggagttcgat
     3661 gagatggaag agtgctctca gcactatccc tacgatgtgc ccgattacgc tggcgcgtcg
     3721 tctgcatgcc ccaagaagaa gaggaaggtg tcgccaggga tccgtcgact tgacgcgttg
     3781 atatcaacaa gtttgtacaa aaaagcaggc tacaaagagg ccagcggttc cggacgggct
     3841 gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac
     3901 cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac
     3961 gcccttgatg atttcgacct ggacatgctg attaactcta gaagttccgg atctccgaaa
     4021 aagaaacgca aagttggtag ccagtacctg cccgacaccg acgaccggca ccggatcgag
     4081 gaaaagcgga agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc
     4141 ggccccaccg accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc
     4201 agcgtgccaa aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac
     4261 tacgacgagt tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg
     4321 gctccagccc ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg
     4381 gtgtctgcac tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag
     4441 gctgtggctc caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct
     4501 ctgctgcagc tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat
     4561 cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac
     4621 cagggcatcc ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc
     4681 atcacccggc tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga
     4741 gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgat
     4801 atggatttct cagccttgct gtgatctaga gggcccggta ttgcggtacc cttgtacgcc
     4861 tgttttatac tcccttcccg taacttagac gcacaaaacc aagttcaata gaagggggta
     4921 caaaccagta ccaccacgaa caagcacttc tgtttccccg gtgatgtcgt atagactgct
     4981 tgcgtggttg aaagcgacgg atccgttatc cgcttatgta cttcgagaag cccagtacca
     5041 cctcggaatc ttcgatgcgt tgcgctcagc actcaacccc agagtgtagc ttaggctgat
     5101 gagtctggac atccctcacc ggtgacggtg gtccaggctg cgttggcggc ctacctatgg
     5161 ctaacgccat gggacgctag ttgtgaacaa ggtgtgaaga gcctattgag ctacataaga
     5221 atcctccggc ccctgaatgc ggctaatccc aacctcggag caggtggtca caaaccagtg
     5281 attggcctgt cgtaacgcgc aagtccgtgg cggaaccgac tactttgggt gtccgtgttt
     5341 ccttttattt tattgtggct gcttatggtg acaatcacag attgttatca taaagcgaat
     5401 tggataggat ccgtacgatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     5461 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     5521 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     5581 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     5641 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     5701 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     5761 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     5821 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     5881 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     5941 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6001 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6061 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6121 acaagtaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6181 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6241 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6301 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6361 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6421 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6481 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6541 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6601 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6661 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6721 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6781 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6841 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     6901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6961 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7021 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7081 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7141 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7201 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgatgaa
     7261 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     7321 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     7381 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     7441 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     7501 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     7561 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     7621 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     7681 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     7741 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     7801 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     7861 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     7921 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     7981 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     8041 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     8101 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     8161 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     8221 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     8281 gcacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     8341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     8401 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8521 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     8581 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     8641 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     8701 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8761 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8821 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9241 cgaacccccc gttcagcccg accgct
//
