LOCUS Exported 9440 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.. ACCESSION . VERSION . KEYWORDS rTetR-NS3-VP64-p65_IRES_mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9440) AUTHORS Tague EP, Dotson HL, Tunney SN, Sloas DC, Ngo JT TITLE Chemogenetic control of gene expression and cell signaling with antiviral drugs. JOURNAL Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2. PUBMED 29967495 REFERENCE 2 (bases 1 to 9440) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9440 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(476..1336) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1099..1118 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1337..1441) /gene="bla" /label=AmpR promoter primer_bind complement(1516..1535) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 1707..2086 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2087..2290 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2241..2261 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2335..2354 /label=T7 /note="T7 promoter, forward primer" promoter 2335..2353 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3859..3885 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 3904..3924 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" protein_bind 3961..3985 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 4015..4164 /codon_start=1 /product="tetrameric repeat of the minimal activation domain of herpes simplex virus VP16 (Beerli et al., 1998)" /label=VP64 /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF DLDML" CDS 4189..4209 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4639..4995 /codon_start=1 /product="transcriptional activation domain of human RelA, also known as p65 (O'Shea and Perkins, 2008)" /label=RelA (p65) AD /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD EDFSSIADMDFSALL" CDS 5592..6302 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(5737..5755) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 6005..6024 /label=mCherry-F /note="mCherry, forward primer" primer_bind complement(6334..6351) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6340..6564 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6610..7038 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6697..6716) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6907..6928 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7047..7067) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7052..7381 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7232..7367 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7294..7313 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7430..8455 /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" polyA_signal 8585..8706 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8622..8641) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8676..8695 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8755..8771) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8755..8771) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8768..8790) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8779..8795 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8803..8833) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8848..8869 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8986..9003) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9157..305) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9237..9256) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 61 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 121 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 181 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 241 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 601 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 781 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 841 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 901 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 961 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 1021 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 1081 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 1141 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 1201 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 1261 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 1321 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 1381 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 1441 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 1501 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 1561 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 1621 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 1681 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 1741 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 1801 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 1861 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 1921 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 1981 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 2041 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 2101 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 2161 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 2221 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 2281 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 2341 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatgtct 2401 agactggaca agagcaaagt cataaacgga gctctggaat tactcaatgg tgtcggtatc 2461 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac 2521 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg 2581 catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac 2641 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc 2701 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag 2761 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg 2821 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc 2881 accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg gcagggagcc 2941 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag 3001 tgcgaaagcg gggcgtctgc aggcatggcc agcatgactg gtggacagca aatggggtcg 3061 acggaggacg tggtgtgctg ccactcaatc tacggcaaga agaagggtga tatcgacacc 3121 taccgataca taggctcttc cgggacaggc tgcgtggtca tagtgggcag gatcgtcttg 3181 tccggatccg gcactagtgc gcccatcacg gcgtacgccc agcagacgag aggcctccta 3241 gggtgtataa tcaccagcct gactggccgg gacaaaaacc aagtggaggg tgaggtccag 3301 atcgtgtcaa ctgctaccca aaccttcctg gcaacgtgca tcaatggggt atgctgggca 3361 gtctaccacg gggccggaac gaggaccatc gcatcaccca agggtcctgt catccagatg 3421 tataccaatg tggaccaaga ccttgtgggc tggcccgctc ctcaaggttc ccgctcattg 3481 acaccctgta cctgcggctc ctcggacctt tacctggtca cgaggcacgc cgatgtcatt 3541 cccgtgcgcc ggcgaggtga tagcaggggt agcctgcttt cgccccggcc catttcctac 3601 ttgaaaggct cctcgggggg tccgctgttg tgccccgcgg gacacgccgt gggcctattc 3661 agggccgcgg tgtgcacccg tggagtggct aaagcggtgg actttatccc tgtggagaac 3721 ctagagacaa ccatgagatc cccggtgttc acggacaact cctctccacc agcagtcacc 3781 ctgacgcacc caatcaccaa aatcgatagg gaggttctct accaggagtt cgatgagatg 3841 gaagagtgct ctcagcacta tccctacgat gtgcccgatt acgctggcgc gtcgtctgca 3901 tgccccaaga agaagaggaa ggtgtcgcca gggatccgtc gacttgacgc gttgatatca 3961 acaagtttgt acaaaaaagc aggctacaaa gaggccagcg gttccggacg ggctgacgca 4021 ttggacgatt ttgatctgga tatgctggga agtgacgccc tcgatgattt tgaccttgac 4081 atgcttggtt cggatgccct tgatgacttt gacctcgaca tgctcggcag tgacgccctt 4141 gatgatttcg acctggacat gctgattaac tctagaagtt ccggatctcc gaaaaagaaa 4201 cgcaaagttg gtagccagta cctgcccgac accgacgacc ggcaccggat cgaggaaaag 4261 cggaagcgga cctacgagac attcaagagc atcatgaaga agtccccctt cagcggcccc 4321 accgacccta gacctccacc tagaagaatc gccgtgccca gcagatccag cgccagcgtg 4381 ccaaaacctg ccccccagcc ttaccccttc accagcagcc tgagcaccat caactacgac 4441 gagttcccta ccatggtgtt ccccagcggc cagatctctc aggcctctgc tctggctcca 4501 gcccctcctc aggtgctgcc tcaggctcct gctcctgcac cagctccagc catggtgtct 4561 gcactggctc aggcaccagc acccgtgcct gtgctggctc ctggacctcc acaggctgtg 4621 gctccaccag cccctaaacc tacacaggcc ggcgagggca cactgtctga agctctgctg 4681 cagctgcagt tcgacgacga ggatctggga gccctgctgg gaaacagcac cgatcctgcc 4741 gtgttcaccg acctggccag cgtggacaac agcgagttcc agcagctgct gaaccagggc 4801 atccctgtgg cccctcacac caccgagccc atgctgatgg aataccccga ggccatcacc 4861 cggctcgtga caggcgctca gaggcctcct gatccagctc ctgcccctct gggagcacca 4921 ggcctgccta atggactgct gtctggcgac gaggacttca gctctatcgc cgatatggat 4981 ttctcagcct tgctgtgatc tagagggccc ggtattgcgg tacccttgta cgcctgtttt 5041 atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg ggtacaaacc 5101 agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg 5161 gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt accacctcgg 5221 aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc tgatgagtct 5281 ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct atggctaacg 5341 ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat aagaatcctc 5401 cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc agtgattggc 5461 ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt gtttcctttt 5521 attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc gaattggata 5581 ggatccgtac gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 5641 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 5701 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 5761 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 5821 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 5881 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 5941 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 6001 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 6061 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 6121 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 6181 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 6241 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 6301 aatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 6361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 6421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 6481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 6541 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 6601 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 6661 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 6721 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 6781 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 6841 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 6901 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 6961 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 7021 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 7081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 7141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 7201 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 7261 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 7321 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 7381 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtga tgaaaaagcc 7441 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga 7501 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg 7561 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta 7621 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag 7681 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc 7741 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc 7801 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata 7861 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac 7921 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg 7981 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt 8041 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga 8101 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca 8161 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc 8221 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga 8281 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt 8341 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt 8401 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagcacgt 8461 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 8521 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 8581 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 8641 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 8701 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 8761 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 8821 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 8881 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 8941 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 9001 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 9061 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 9121 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 9181 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 9241 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 9301 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 9361 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 9421 ccccgttcag cccgaccgct //