LOCUS Exported 12482 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION express wild-type NSD2 in mammalian cells. ACCESSION . VERSION . KEYWORDS LVXN-Neo-NSD2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12482) AUTHORS Jaffe JD, Wang Y, Chan HM, Zhang J, Huether R, Kryukov GV, Bhang HE, Taylor JE, Hu M, Englund NP, Yan F, Wang Z, Robert McDonald E 3rd, Wei L, Ma J, Easton J, Yu Z, deBeaumount R, Gibaja V, Venkatesan K, Schlegel R, Sellers WR, Keen N, Liu J, Caponigro G, Barretina J, Cooke VG, Mullighan C, Carr SA, Downing JR, Garraway LA, Stegmeier F TITLE Global chromatin profiling reveals NSD2 mutations in pediatric acute lymphoblastic leukemia. JOURNAL Nat Genet. 2013 Nov;45(11):1386-91. doi: 10.1038/ng.2777. Epub 2013 Sep 29. PUBMED 24076604 REFERENCE 2 (bases 1 to 12482) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12482 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 125..758 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 805..930 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1427..1660 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1845..1889 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2152..2267 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" enhancer 2325..2628 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2629..2832 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2783..2803 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2829..2853 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2937..2951 /codon_start=1 /product="enterokinase recognition and cleavage site" /label=enterokinase site /translation="DDDDK" protein_bind 2954..2978 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 2987..2996 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2996..3019 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS 5698..5709 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(7142..7166) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" misc_feature 7180..7753 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(7347..7364) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 7574..7593 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 7767..8561 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7821..7840) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8431..8450 /label=Neo-F /note="Neomycin resistance gene, forward primer" misc_feature 8575..9163 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8628..8648) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(9046..9057) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9370..10003 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(10131..10147) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10131..10147) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10144..10166) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10155..10171 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10179..10209) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10224..10245 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10362..10379) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10533..11121) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10613..10632) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11292..12152) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11915..11934 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12153..12257) /gene="bla" /label=AmpR promoter polyA_signal 12305..12439 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12342..12361) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12396..12415 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt 61 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag 121 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac 181 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat 241 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag 301 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac 361 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc 421 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt 481 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc 541 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat 601 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 661 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 721 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg 781 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc 841 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc 901 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga 961 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta 1021 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa 1081 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt 1141 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa 1201 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca 1261 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga 1321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 1381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 1441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac 1501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 1561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 1621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 1681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 1741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa 1801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat 1861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt 1921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg 1981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc 2041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga 2101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga 2161 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac 2221 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac 2281 agggacagca gagatccagt ttatcgataa gcttgggagt tccgcgttac ataacttacg 2341 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg 2401 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta 2461 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt 2521 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac 2581 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt 2641 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac 2701 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt 2761 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat 2821 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt 2881 gacctccata gaagacaccg actctactag aggatctatt tccggtgaat tgtaccgacg 2941 acgacgacaa ggcacaagtt tgtacaaaaa agcaggctcc gcggccgcca ccatggacta 3001 caaggacgac gatgacaaag gtggttctgg aggatcagaa tttagcatca agcagagtcc 3061 cctttctgtt cagagtgttg taaagtgcat aaagatgaag caggcaccag aaatcctcgg 3121 cagtgccaac gggaagactc cgagctgcga ggtgaaccgc gagtgttctg tgttcctcag 3181 caaagcccag ctctccagta gcctgcagga gggggtcatg cagaagttta acggccacga 3241 cgccctgccc tttattccag ccgacaagct gaaagatctt acttcccggg tgtttaatgg 3301 agaacccggc gcacacgatg ccaaactgcg ttttgagtcc caggaaatga aagggattgg 3361 gacaccccct aacactaccc ctatcaaaaa tggctctcca gaaattaagc tgaaaatcac 3421 caaaacatac atgaatggga agcctctctt tgaatcttcc atttgtggtg acagtgctgc 3481 tgatgtgtct cagtcagaag aaaatggaca aaaaccagaa aacaaggcga gaaggaacag 3541 gaagaggagc ataaaatatg actccttgct ggagcagggc cttgtcgaag cagctcttgt 3601 gtctaagatc tcaagtcctt cagataaaaa gattccagct aagaaagagt cttgtccaaa 3661 cactggaaga gacaaagacc acctgttgaa atacaacgtt ggtgatttgg tgtggtccaa 3721 agtgtcgggt tacccttggt ggccttgcat ggtttctgca gatccactcc ttcacagcta 3781 taccaaactt aaaggtcaga aaaagagtgc acgccagtat cacgtacagt tctttggtga 3841 cgccccagaa agagcttgga tatttgagaa gagcctcgta gcttttgaag gagaaggaca 3901 gtttgaaaaa ttatgccagg aaagtgccaa gcaggcaccc acgaaagctg agaaaattaa 3961 gctattgaaa ccaatttcag ggaaattgag ggcccagtgg gaaatgggca ttgttcaagc 4021 agaagaagct gcaagcatgt cagtggagga gcggaaagcc aagttcacct ttctctatgt 4081 gggggaccag cttcatctca accctcaagt agccaaggag gctggcattg ctgcagagtc 4141 tttgggagaa atggcagaat cctcaggagt cagtgaagaa gctgctgaaa accccaagtc 4201 tgtgagagaa gagtgcattc ccatgaagag aaggcggagg gccaaactgt gtagctctgc 4261 agagaccctg gagagtcacc ccgacatagg gaagagtact cctcaaaaga cggcagaggc 4321 tgaccccaga agaggagtag ggtctcctcc tgggaggaag aagaccacag tctccatgcc 4381 acgaagcagg aagggagatg cagcatccca gtttttggtc ttctgtcaaa aacacaggga 4441 tgaggtggta gctgagcacc cagatgcttc aggtgaggag attgaagagc tgctcaggtc 4501 acagtggagt ctgctgagtg agaagcagag agcacgctac aacaccaagt ttgccctggt 4561 ggcccctgtc caggctgaag aagactctgg taatgtaaat gggaaaaaaa gaaaccacac 4621 aaagaggata caggacccta cagaagatgc tgaagctgag gacacaccca ggaaaagact 4681 caggacggac aagcacagtc ttcggaagag agacacaatc actgacaaaa cggccagaac 4741 aagctcttac aaggccatgg aggcagcctc ctcgctcaag agccaggcag caacgaaaaa 4801 tctgtctgat gcatgtaaac cactgaagaa gcgaaatcgg gcttccacgg cagcatcttc 4861 agctcttggg tttagcaaaa gttcatctcc ttctgcatcc ttaactgaga atgaggtctc 4921 ggacagcccg ggagacgagc cctcggagtc cccatacgaa agtgcagacg aaacacaaac 4981 tgaagtatct gtctcatcca aaaagtctga gcgaggagtg actgccaaaa aggagtatgt 5041 gtgccagctg tgtgagaagc cgggcagcct cctgctctgt gaaggaccct gctgcggagc 5101 tttccacctc gcctgccttg ggctttcccg gaggccagaa gggaggttca cctgcagcga 5161 gtgtgcctca gggattcact catgtttcgt gtgtaaagag agcaagacag atgttaagcg 5221 ctgtgtggta actcagtgtg gaaaatttta ccatgaggct tgtgtgaaaa aataccctct 5281 gactgtattt gagagccgag gtttccgctg ccccctccac agctgtgtga gctgccatgc 5341 ttccaaccct tcaaacccaa ggccgtcaaa aggtaaaatg atgcggtgtg tccgctgccc 5401 cgttgcctat cacagcgggg atgcttgtct ggcagcagga tgctcagtga tcgcctccaa 5461 cagcatcatc tgcactgccc acttcactgc tcggaagggg aagcgacacc acgcccacgt 5521 caacgtgagc tggtgcttcg tgtgctccaa aggggggagc cttctgtgct gtgagtcctg 5581 cccagcggcc ttccaccctg actgcctgaa catcgagatg cctgacggca gctggttctg 5641 caatgactgc agggctggga agaagctgca cttccaggat atcatttggg tgaaacttgg 5701 gaactacaga tggtggccgg cagaagtttg ccatcccaaa aatgttcccc caaatattca 5761 gaaaatgaag cacgagattg gagaattccc tgtgtttttc tttgggtcta aagattatta 5821 ctggacgcat caggcgcgag tgttcccgta catggagggg gaccggggca gccgctacca 5881 gggggtcaga gggatcggaa gagtcttcaa aaacgcactg caagaagctg aagctcgttt 5941 tcgtgaaatt aagcttcaga gggaagcccg agaaacacag gagagcgagc gcaagccccc 6001 accatacaag cacatcaagg tcaacaaacc ctatggccgc gtccagatct acacagcgga 6061 tatttcagaa atccctaagt gcaactgcaa gcccacagat gagaatcctt gtggctttga 6121 ttcggagtgt ctgaacagga tgctgatgtt tgagtgccac ccgcaggtgt gtcccgcggg 6181 cgagttctgc cagaaccagt gcttcaccaa gcgccagtac ccagagacca agatcatcaa 6241 gacagatggc aaagggtggg gcctggtcgc caagagggac atcagaaagg gagaatttgt 6301 taacgagtac gttggggagc tgatcgacga ggaggagtgc atggcgagaa tcaagcacgc 6361 acacgagaac gacatcaccc acttctacat gctcactata gacaaggacc gtataataga 6421 cgctggcccc aaaggaaact actctcgatt tatgaatcac agctgccagc ccaactgtga 6481 gaccctcaag tggacagtga atggggacac tcgtgtgggc ctgtttgccg tctgtgacat 6541 tcctgcaggg acggagctga cttttaacta caacctcgat tgtctgggca atgaaaaaac 6601 ggtctgccga tgtggagcct ccaattgcag tggattcctc ggggatagac caaagacctc 6661 gacgaccctt tcatcagagg aaaagggcaa aaagaccaag aagaaaacga ggcggcgcag 6721 agcaaaaggg gaagggaaga ggcagtcaga ggacgagtgc ttccgctgcg gtgatggcgg 6781 gcagctggtg ctgtgtgacc gcaagttctg caccaaggcc taccacctgt cctgcctggg 6841 ccttggcaag cggcccttcg ggaagtggga atgtccttgg catcattgtg acgtgtgtgg 6901 gaagccctct acctctttct gccacctctg ccccaattcg ttctgtaagg agcaccagga 6961 cgggacagcc ttcagctgca ccccggacgg gcggtcctac tgctgtgagc atgacttagg 7021 ggcggcatcg gtcagaagca ccaagactga gaagcccccc ccagagccag ggaagccgaa 7081 ggggaagagg cggcggcgga ggggctggcg gagagtcaca gagggcaaat gaggcgcgcc 7141 gacccagctt tcttgtacaa agtggttgcg atgatcccgc ccctctccct cccccccccc 7201 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt 7261 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt 7321 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt 7381 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct 7441 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt 7501 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt 7561 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa 7621 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta 7681 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa 7741 aacacgatga taagcttgcc acaaccatgg ctgaacaaga tggattgcac gcaggttctc 7801 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 7861 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 7921 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca 7981 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 8041 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 8101 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 8161 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 8221 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 8281 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 8341 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 8401 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 8461 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 8521 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aacgcgtctg gaacaatcaa 8581 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt 8641 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct 8701 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 8761 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg 8821 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc 8881 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc 8941 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt 9001 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 9061 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt 9121 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca 9181 gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 9241 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 9301 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 9361 agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 9421 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca 9481 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag 9541 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg 9601 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg 9661 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg 9721 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag 9781 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac 9841 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 9901 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 9961 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 10021 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga 10081 cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 10141 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 10201 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 10261 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 10321 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 10381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 10441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 10501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 10561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 10621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 10681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 10741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 10801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 10861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 10921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 10981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 11041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 11101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 11161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 11221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 11281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 11341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 11401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 11461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 11521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 11581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 11641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 11701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 11761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 11821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 11881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 11941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 12001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 12061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 12121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 12181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 12241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga 12301 gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 12361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 12421 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc 12481 ca //