LOCUS       Exported               11583 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway entry vector carrying human EF1a promoter-driven 
            dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker.
ACCESSION   .
VERSION     .
KEYWORDS    pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Chong ZS, Ohnishi S, Yusa K, Wright GJ
  TITLE     Pooled extracellular receptor-ligand interaction screening using 
            CRISPR activation.
  JOURNAL   Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3.
  PUBMED    30477585
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        552..1732
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          782..1724
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1681..1701
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1763..1810
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             1811..5911
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5966..5986
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6011..6160
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6191..6244
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6251..6260
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6260..6646
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             6701..6721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6737..7279
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             7304..7675
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             7713..7733
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7758..8344
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7925..7942)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8152..8171
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8345..9148
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8408..8427)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9018..9037
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9180..9197)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9186..9410
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(9455..9554)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9666..9685)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9677..10486
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9744..9763)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10576..11164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11065..11084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      11329..11415
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      11507..11534
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     11542..11565
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt
      121 cgactggatc cacgcgtgag gggcgcggaa gccggggcgg aggaggagcc aaccccgggc
      181 tgggctgaga cccgcagagg aagacgctct agggatttgt cccggactag cgagatggca
      241 aggctgagga cgggaggctg attgagaggc gaaggtacac cctaatctca atacaacctt
      301 tggagctaag ccagcaatgg tagagggaag attctgcacg tcccttccag gcggcctccc
      361 cgtcaccacc ccccccaacc cgccccgacc ggagctgaga gtaattcata caaaaggact
      421 cgcccctgcc ttggggaatc ccagggaccg tcgttaaact cccactaacg tagaacccag
      481 agatcgctgc gttcccgccc cctcacccgc ccgctctcgt catcactgag gtggagaaga
      541 gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      601 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaagtggc gcggggtaaa
      661 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      721 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      781 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
      841 gccttgaatt acttccacgc ccctggctgc agtacgtgat tcttgatccc gagcttcggg
      901 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
      961 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     1021 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     1081 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     1141 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     1201 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     1261 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     1321 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     1381 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     1441 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     1501 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     1561 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     1621 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     1681 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tggaagcttg
     1741 gcgcgcccgt acggccacca tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     1801 aaagaaaaag gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg
     1861 ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac
     1921 cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac
     1981 agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg
     2041 gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt
     2101 ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat
     2161 cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct
     2221 gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct
     2281 ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa
     2341 cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga
     2401 aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa
     2461 gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt
     2521 cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct
     2581 ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct
     2641 gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga
     2701 cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag
     2761 cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct
     2821 cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg
     2881 ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc
     2941 catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct
     3001 gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga
     3061 gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga
     3121 aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg
     3181 aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt
     3241 cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt
     3301 cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt
     3361 caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc
     3421 cttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa
     3481 agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt
     3541 ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct
     3601 gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga
     3661 tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac
     3721 ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg
     3781 ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac
     3841 aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca
     3901 cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga
     3961 tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct
     4021 gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa
     4081 catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg
     4141 cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga
     4201 acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa
     4261 tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt
     4321 ggaccacatc gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac
     4381 cagaagcgac aaggcccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa
     4441 gatgaagaac tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga
     4501 caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa
     4561 gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg
     4621 gatgaacact aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct
     4681 gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat
     4741 caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat
     4801 caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt
     4861 gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt
     4921 ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg
     4981 gaagcggcct ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg
     5041 ggattttgcc accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac
     5101 cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa
     5161 gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac
     5221 cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa
     5281 gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc
     5341 catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct
     5401 gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg
     5461 cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct
     5521 ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt
     5581 tgtggaacag cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa
     5641 gagagtgatc ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg
     5701 ggataagccc atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct
     5761 gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag
     5821 caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac
     5881 acggatcgac ctgtctcagc tgggaggcga cagcgctgga ggaggtggaa gcggaggagg
     5941 aggaagcgga ggaggaggta gcggacctaa gaaaaagagg aaggtggcgg ccgctggatc
     6001 cggacgggct gacgcattgg acgattttga tctggatatg ctgggaagtg acgccctcga
     6061 tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc tcgacatgct
     6121 cggcagtgac gcccttgatg atttcgacct ggacatgctg attaactgta cgggcggcgg
     6181 gtccggagga gagggcagag gaagtctcct aacatgcggt gacgtggagg agaatcctgg
     6241 cccatgtacg gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg
     6301 gacaggggat gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag
     6361 ctccaactca cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca
     6421 gaagagaaag tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg
     6481 agtcgaactg cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat
     6541 tttcgctacc aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga
     6601 cggtaatcct atcccttccg ccatcgccgc taactcaggt atctacagcg ctggaggagg
     6661 tggaagcgga ggaggaggaa gcggaggagg aggtagcgga cctaagaaaa agaggaaggt
     6721 ggcggccgct ggatcccctt cagggcagat cagcaaccag gccctggctc tggcccctag
     6781 ctccgctcca gtgctggccc agactatggt gccctctagt gctatggtgc ctctggccca
     6841 gccacctgct ccagcccctg tgctgacccc aggaccaccc cagtcactga gcgctccagt
     6901 gcccaagtct acacaggccg gcgaggggac tctgagtgaa gctctgctgc acctgcagtt
     6961 cgacgctgat gaggacctgg gagctctgct ggggaacagc accgatcccg gagtgttcac
     7021 agatctggcc tccgtggaca actctgagtt tcagcagctg ctgaatcagg gcgtgtccat
     7081 gtctcatagt acagccgaac caatgctgat ggagtacccc gaagccatta cccggctggt
     7141 gaccggcagc cagcggcccc ccgaccccgc tccaactccc ctgggaacca gcggcctgcc
     7201 taatgggctg tccggagatg aagacttctc aagcatcgct gatatggact ttagtgccct
     7261 gctgtcacag atttcctcta gtgggcaggg aggaggtgga agcggcttca gcgtggacac
     7321 cagtgccctg ctggacctgt tcagcccctc ggtgaccgtg cccgacatga gcctgcctga
     7381 ccttgacagc agcctggcca gtatccaaga gctcctgtct ccccaggagc cccccaggcc
     7441 tcccgaggca gagaacagca gcccggattc agggaagcag ctggtgcact acacagcgca
     7501 gccgctgttc ctgctggacc ccggctccgt ggacaccggg agcaacgacc tgccggtgct
     7561 gtttgagctg ggagagggct cctacttctc cgaaggggac ggcttcgccg aggaccccac
     7621 catctccctg ctgacaggct cggagcctcc caaagccaag gaccccactg tctcctgtac
     7681 atgagaattc gcggccgcaa ggtggaggtg gacccaagaa gaagcgcaag gtgtgagtta
     7741 acggccgccg cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc
     7801 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     7861 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     7921 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     7981 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     8041 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     8101 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     8161 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     8221 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     8281 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     8341 aaccatggga tcggccattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     8401 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     8461 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8521 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8581 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8641 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     8701 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     8761 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8821 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8881 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8941 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     9001 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     9061 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     9121 ctatcgcctt cttgacgagt tcttctgacg tacgcgatga tctagagctc gctgatcagc
     9181 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     9241 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     9301 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     9361 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     9421 ggaaagaacc tgcaactagt ctcgagatat ctagacccag ctttcttgta caaagttggc
     9481 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     9541 aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc tctgatgtta
     9601 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     9661 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     9721 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     9781 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     9841 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     9901 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     9961 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
    10021 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
    10081 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
    10141 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
    10201 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
    10261 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
    10321 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
    10381 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
    10441 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
    10501 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa
    10561 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10621 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10681 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10741 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10801 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10861 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10921 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10981 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11041 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11101 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    11161 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    11221 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ctagcatgga
    11281 tctcggggac gtctaactac taagcgagag tagggaactg ccaggcatca aataaaacga
    11341 aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
    11401 ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg gcccggaggg
    11461 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg
    11521 acggatggcc tttttgcgtt tctacaaact cttcctgtta gttagttact taagctcggg
    11581 ccc
//
