LOCUS Exported 10817 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Targeted DNA methylation. ACCESSION . VERSION . KEYWORDS pSt1374-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10817) AUTHORS Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD TITLE Locus-specific DNA methylation of Mecp2 promoter leads to autism-like phenotypes in mice. JOURNAL Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x. PUBMED 32015323 REFERENCE 2 (bases 1 to 10817) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10817 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 676..696 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1351..1368 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 6385..6405 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6448..6489 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" CDS 6499..6516 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(6539..6556) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6545..6769 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6815..7243 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6902..6921) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7112..7133 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7252..7272) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7257..7586 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7437..7572 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7499..7518 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7634..7681 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7700..8098 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 8256..8377 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8293..8312) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8347..8366 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8426..8442) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8426..8442) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8439..8461) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8450..8466 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8474..8504) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8519..8540 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8657..8674) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8828..9416) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8908..8927) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9587..10447) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10210..10229 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10448..10552) /gene="bla" /label=AmpR promoter primer_bind complement(10627..10646) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagcacca tgggacctaa gaaaaagagg aaggtggcgg ccgctggcgg cagcatgttc 721 gaaaccgtgc ctgtgtggag gagacagcca gtccgggtgc tgtccctttt tgaagacatc 781 aagaaagagc tgacgagttt gggctttttg gaaagtggtt ctgacccggg acaactgaag 841 catgtggttg atgtcacaga cacagtgagg aaggatgtgg aggagtgggg acccttcgat 901 cttgtgtacg gcgccacacc tcccctgggc cacacctgtg accgtcctcc cagctggtac 961 ctgttccagt tccaccggct cctgcagtac gcacggccca agccaggcag ccccaggccc 1021 ttcttctgga tgttcgtgga caatctggtg ctgaacaagg aagacctgga cgtcgcatct 1081 cgcttcctgg agatggagcc agtcaccatc ccagatgtcc acggcggatc cttgcagaat 1141 gctgtccgcg tgtggagcaa catcccagcc ataaggagca gcaggcactg ggctctggtt 1201 tcggaagaag aattgtccct gctggcccag aacaagcaga gctcgaagct cgcggccaag 1261 tggcccacca agctggtgaa gaactgcttt ctccccctaa gagaatattt caagtatttt 1321 tcaagcagcg ggcgcagctt cagcagcgga ctagtgcccc gcggcagcca cccagctgag 1381 aagaggaagc ccatccgggt gctgtctctc tttgatggaa tcgctacagg gctcctggtg 1441 ctgaaggact tgggcattca ggtggaccgc tacattgcct cggaggtgtg tgaggactcc 1501 atcacggtgg gcatggtgcg gcaccagggg aagatcatgt acgtcgggga cgtccgcagc 1561 gtcacacaga agcatatcca ggagtggggc ccattcgatc tggtgattgg gggcagtccc 1621 tccaatgacc tctccatcgt caaccctgct cgcaagggcc tctacgaggg cactggccgg 1681 ctcttctttg agttctaccg cctcctgcat gatgcgcggc ccaaggaggg agatgatcgc 1741 cccttcttct ggctctttga gaatgtggtg gccatgggcg ttagtgacaa gagggacatc 1801 tcgcgatttc tcgagtccaa ccctgtgatg attgatgcca aagaagtgtc agctgcacac 1861 agggcccgct acttctgggg taaccttccc ggtatgaaca ggccgttggc atccactgtg 1921 aatgataagc tggagctgca ggagtgtctg gagcatggca ggatagccaa gttcagcaaa 1981 gtgaggacca ttactacgag gtcaaactcc ataaagcagg gcaaagacca gcattttcct 2041 gtcttcatga atgagaaaga ggacatctta tggtgcactg aaatggaaag ggtatttggt 2101 ttcccagtcc actatactga cgtctccaac atgagccgct tggcgaggca gagactgctg 2161 ggccggtcat ggagcgtgcc agtcatccgc cacctcttcg ctccgctgaa ggagtatttt 2221 gcgtgtgtga gcggcagcga gacccccggt accagcgaga gcgccacccc cgagagcgac 2281 aagaaatact ctattggact ggctatcggg acaaactccg ttggctgggc cgtcataacc 2341 gacgagtata aggtgccaag caagaaattc aaggtgctgg gtaatactga ccgccattca 2401 atcaagaaga acctgatcgg agcactcctc ttcgactccg gtgaaaccgc tgaagctact 2461 cggctgaagc ggaccgcaag gcggagatac acccgccgca agaatcggat atgttatctg 2521 caagagatct ttagcaacga aatggctaag gtggacgact ccttctttca ccgcctggaa 2581 gagagctttc tggtggagga ggataagaaa cacgagaggc accctatatt cggaaatatc 2641 gtggatgagg tggcttacca tgaaaagtat cctacaatct accatctgag gaagaagctg 2701 gtggacagca ccgataaagc agacctgagg ctcatctatc tggccctggc tcatatgata 2761 aagtttagag gacactttct gatcgagggc gacctgaatc ccgataattc cgatgtggat 2821 aaactcttca ttcaactggt gcagacatat aaccaactgt tcgaggagaa tcccataaac 2881 gcttctggtg tggatgccaa ggctattctg tccgctcggc tgtccaagtc acgcagactg 2941 gagaatctga ttgcccaact gccaggagaa aagaagaacg gcctgtttgg gaacctcatc 3001 gccctgagcc tgggcctgac acctaacttc aagtccaatt ttgatctggc cgaagatgct 3061 aaactccagc tctccaagga cacctatgac gatgatctgg acaacctgct cgcacagata 3121 ggcgaccagt acgccgatct ctttctggct gctaagaatc tctccgacgc cattctgctg 3181 agcgacatac tccgggtcaa cactgagatc accaaagcac ctctgagcgc ctccatgata 3241 aaacgctatg atgaacacca tcaagacctg actctgctca aagccctcgt gaggcaacag 3301 ctgccagaga agtacaaaga gatattcttc gaccagagca agaatggata tgccggatac 3361 atcgatggcg gagcatcaca ggaagaattt tacaagttca tcaaaccaat cctcgagaag 3421 atggacggta ctgaagagct gctggtgaag ctgaacaggg aggacctgct gaggaagcag 3481 aggacctttg ataatggctc cattccacat cagatacacc tgggagagct gcatgcaatc 3541 ctccgcaggc aggaggattt ctatcctttc ctgaaggata accgggagaa gatagagaag 3601 atcctgacct tcaggatccc ttattacgtc ggccctctgg ctagaggcaa ctcccgcttc 3661 gcttggatga ccaggaaatc tgaggagaca attactcctt ggaacttcga agaggtcgtg 3721 gataagggcg caagcgccca gtcattcatc gaacggatga ccaatttcga taagaacctg 3781 ccaaacgaga aggtcctgcc caaacattca ctcctgtacg agtatttcac cgtctataac 3841 gagctgacta aagtgaagta cgtgaccgag ggcatgagga agcctgcctt cctgtccgga 3901 gagcagaaga aggctatcgt tgatctgctc ttcaagacta atagaaaggt gacagtgaag 3961 cagctcaagg aggattactt taagaagatc gaatgctttg actcagtgga aatctctggc 4021 gtggaggacc gctttaatgc cagcctgggc acttaccatg atctgctgaa gataatcaaa 4081 gacaaagatt tcctcgataa tgaggagaac gaggacatcc tggaagatat cgtgctgacc 4141 ctgactctgt tcgaggatag agagatgatc gaagagcgcc tgaagaccta tgcccatctg 4201 tttgacgata aagtcatgaa acagctcaag cggcggcgct acactgggtg gggtagactc 4261 tccaggaaac tcataaacgg catccgcgac aaacagagcg gaaagaccat cctggatttc 4321 ctgaaatccg acggattcgc taacaggaac ttcatgcaac tgattcacga tgactctctg 4381 acatttaaag aggacatcca gaaggcacag gtgagcggtc aaggcgacag cctgcacgag 4441 cacatcgcca acctcgctgg atcacccgcc ataaagaagg gaatactgca gacagtcaag 4501 gtcgtggacg aactcgtcaa agtgatgggt cggcacaagc cagagaatat cgttatcgaa 4561 atggcaaggg agaaccaaac cacccagaag ggccagaaga actctcggga acggatgaaa 4621 agaatcgaag agggaattaa ggagctggga tctcagatac tgaaggagca ccctgtggag 4681 aatacacagc tccagaacga gaaactctac ctgtactacc tccagaacgg gcgggacatg 4741 tacgttgacc aggaactcga catcaaccgg ctgtccgatt atgacgtgga cgctattgtt 4801 ccacagtcct tcctcaaaga tgactccatt gacaacaagg tgctgaccag atccgataag 4861 gcccgcggta agtctgacaa tgttccatca gaagaggtgg tcaagaagat gaagaattac 4921 tggcggcagc tcctcaacgc caaactgatc acccagcgga agtttgacaa tctgactaag 4981 gcagaaagag gaggtctgag cgaactcgac aaggccggct ttattaagag gcaactggtc 5041 gaaacacgcc agattaccaa acacgtggca caaatcctcg actctaggat gaacactaag 5101 tacgatgaga acgataagct gatcagggaa gtgaaagtga taactctgaa gagcaagctg 5161 gtgtctgact tccggaagga ctttcaattc tacaaagttc gcgaaataaa caattaccat 5221 catgctcacg atgcctatct caatgctgtc gttggcaccg ccctgatcaa gaaataccct 5281 aaactggagt ctgagttcgt gtacggtgac tataaagtct acgatgtgag gaagatgata 5341 gcaaagtctg agcaagagat tggcaaagcc accgccaagt acttcttcta ctctaatatc 5401 atgaatttct ttaagactga gataaccctg gctaacggcg aaatccggaa gcgcccactg 5461 atcgaaacaa acggagaaac aggagaaatc gtgtgggata aaggcaggga cttcgcaact 5521 gtgcggaagg tgctgtccat gccacaagtc aatatcgtga agaagaccga agtgcagacc 5581 ggcggattct caaaggagag catcctgcca aagcggaact ctgacaagct gatcgccagg 5641 aagaaagatt gggacccaaa gaagtatggc ggtttcgatt cccctacagt ggcttattcc 5701 gttctggtcg tggcaaaagt ggagaaaggc aagtccaaga aactcaagtc tgttaaggag 5761 ctgctcggaa ttactattat ggagagatcc agcttcgaga agaatccaat cgatttcctg 5821 gaagctaagg gctataaaga agtgaagaaa gatctcatca tcaaactgcc caagtactct 5881 ctctttgagc tggagaatgg taggaagcgg atgctggcct ccgccggaga gctgcagaaa 5941 ggaaacgagc tggctctgcc ctccaaatac gtgaacttcc tgtatctggc ctcccactac 6001 gagaaactca aaggtagccc tgaagacaat gagcagaagc aactctttgt tgagcaacat 6061 aaacactacc tggacgaaat cattgaacag attagcgagt tcagcaagcg ggttattctg 6121 gccgatgcaa acctcgataa agtgctgagc gcatataata agcacaggga caagccaatt 6181 cgcgaacaag cagagaatat tatccacctc tttactctga ctaatctggg cgctcctgct 6241 gccttcaagt atttcgatac aactattgac aggaagcggt acacctctac caaagaagtt 6301 ctcgatgcca ccctgataca ccagtcaatt accggactgt acgagactcg catcgacctg 6361 tctcagctcg gcggcgacgg ttctcccaag aagaagagga aagtctcgag cggtggagct 6421 gcaggatagg aattcgggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc 6481 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc 6541 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 6601 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 6661 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 6721 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 6781 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 6841 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 6901 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 6961 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 7021 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 7081 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 7141 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 7201 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 7261 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 7321 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 7381 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 7441 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 7501 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 7561 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 7621 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga 7681 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga 7741 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc 7801 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg 7861 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac 7921 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg 7981 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca 8041 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc 8101 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct 8161 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 8221 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg 8281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 8341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct 8401 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 8461 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 8521 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 8581 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 8641 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 8701 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 8761 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 8821 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 8881 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 8941 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 9001 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 9061 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 9121 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 9181 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 9241 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 9301 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 9361 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 9421 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 9481 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 9541 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 9601 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 9661 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 9721 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 9781 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 9841 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 9901 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 9961 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 10021 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 10081 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 10141 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 10201 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 10261 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 10321 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 10381 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 10441 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 10501 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 10561 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac 10621 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt 10681 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac 10741 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg 10801 ggccagatat acgcgtt //