LOCUS       Exported               16785 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Used to make transgenic zebrafish expressing human 4R/0N Tau under 
            the neuronal eno2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pBS-I-Sce1-eno2:Tau-IRES-egfp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16785)
  AUTHORS   Bai Q, Garver JA, Hukriede NA, Burton EA
  TITLE     Generation of a transgenic zebrafish model of Tauopathy using a 
            novel promoter element derived from the zebrafish eno2 gene.
  JOURNAL   Nucleic Acids Res. 2007;35(19):6501-16. Epub 2007 Sep 25.
  PUBMED    17897967
REFERENCE   2  (bases 1 to 16785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1160..1746
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1327..1344)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1554..1573
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1747..2466
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1747..2463
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1792..1813)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2053..2072)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2400..2421
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2589..2670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2626..2645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2680..2699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(2845..2863)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2885..2901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2885..2901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2898..2920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2909..2925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2933..2963)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2978..2999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3116..3133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3287..3875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3367..3386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4046..4906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4669..4688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4907..5011)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5038..5494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5125..5144)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5335..5356
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5620..5642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5634..5651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5635..5651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5673..5692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5673..5691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atggctgagc cccgccagga gttcgaagtg atggaagatc acgctgggac gtacgggttg
       61 ggggacagga aagatcaggg gggctacacc atgcaccaag accaagaggg tgacacggac
      121 gctggcctga aagctgaaga agcaggcatt ggagacaccc ccagcctgga agacgaagct
      181 gctggtcacg tgacccaagc tcgcatggtc agtaaaagca aagacgggac tggaagcgat
      241 gacaaaaaag ccaagggggc tgatggtaaa acgaagatcg ccacaccgcg gggagcagcc
      301 cctccaggcc agaagggcca ggccaacgcc accaggattc cagcaaaaac cccgcccgct
      361 ccaaagacac cacccagctc tggtgaacct ccaaaatcag gggatcgcag cggctacagc
      421 agccccggct ccccaggcac tcccggcagc cgctcccgca ccccgtccct tccaacccca
      481 cccacccggg agcccaagaa ggtggcagtg gtccgtactc cacccaagtc gccgtcttcc
      541 gccaagagcc gcctgcagac agcccccgtg cccatgccag acctgaagaa tgtcaagtcc
      601 aagatcggct ccactgagaa cctgaagcac cagccgggag gcgggaaggt gcagataatt
      661 aataagaagc tggatcttag caacgtccag tccaagtgtg gctcaaagga taatatcaaa
      721 cacgtcccgg gaggcggcag tgtgcaaata gtctacaaac cagttgacct gagcaaggtg
      781 acctccaagt gtggctcatt aggcaacatc catcataaac caggaggtgg ccaggtggaa
      841 gtaaaatctg agaagcttga cttcaaggac agagtccagt cgaagattgg gtccctggac
      901 aatatcaccc acgtccctgg cggaggaaat aaaaagattg aaacccacaa gctgaccttc
      961 cgcgagaacg ccaaagccaa gacagaccac ggggcggaga tcgtgtacaa gtcgccagtg
     1021 gtgtctgggg acacgtctcc acggcatctc agcaatgtct cctccaccgg cagcatcgac
     1081 atggtagact cgccccagct cgccacgcta gctgacgagg tgtctgcctc cctggccaag
     1141 cagggtttgt gaggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     1201 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     1261 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     1321 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     1381 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     1441 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     1501 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     1561 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     1621 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     1681 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     1741 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1801 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1861 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1921 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1981 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2041 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     2101 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     2161 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     2221 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     2281 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     2341 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     2401 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     2461 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     2521 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2581 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2641 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2701 atgtatcaaa cacggccatt acggccaaac tcgctcgccc gggccgaacc agtggctgca
     2761 gggccgcctc ggccaaaggc ttaattaaag atctttacta gttctagagc ggccgccacc
     2821 gcggtggagc tccagctttt gttcccttta gtgagggtta attaccctgt tatccctaat
     2881 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     2941 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3001 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3061 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4561 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4921 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4981 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     5041 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5101 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5161 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5221 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5281 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5341 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5401 ggattttgcc gatttcggcc tattggttaa aaaaatgagc tgatttaaca aaaatttaac
     5461 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
     5521 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     5581 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     5641 cgacggccag tgaaattacc ctgttatccc tataatacga ctcactatag ggcgaattgg
     5701 gtacctgtta gtaaaggcag attcataata acaaaaaagt aatttcttta aaagttacag
     5761 aaacatcttg ttgtatatca tcattaataa atgagccaat atgtttccaa aattgtttaa
     5821 aatggtagaa ttacacatgt tctatataca ctgtacaaca tatatgtcaa ttaacctttt
     5881 tttatttgtc ttgtgtttac attcactaag tttgccagtt gtttactcct ttggcattgt
     5941 gttatgtgac ctttatgttt atgttaaaaa tggaactctg cagctcaaca aagtaatttt
     6001 tattgccatt acaccaatat gttttataag aagaataaag tacaggcaat ttatatgacc
     6061 aacccagaaa atagactata ttgtttctaa ctatactaac aatattaaca aaaaacttta
     6121 aacatctaat actgcgattg aaaagcataa atcaacagaa aacactcctt aatcacaaac
     6181 gacattttta cagaacgttt acatttcatg tggaaaattt tgggggcact ttataatttt
     6241 gaaactaatg attacttttg tcgatcaaac gtgccattaa agatatttat aactaaacag
     6301 tgaatttcta attcgtataa ttgttgtttt caagttcaca tgaatacaac agaaggtaat
     6361 gtggacttga atgaattaca ctaactggcc actttattag gtacacacgt ccaactgctc
     6421 tttaacacaa atttctaatc agccaatcac gtggcagcaa ctcaatgcaa ccatgttatt
     6481 taagtaaaag gtgattaaag tgactttgaa cgtggcatgt ttgttggcgc cagacgggct
     6541 ggtcggagta tttcagaaat tgctgatcag taactcaaat aaccactcgt tacaaccgag
     6601 gtatgcagaa gagcatctct gaaaacacaa cacgtccaac cttgaggcag atgggctaca
     6661 gcagcagaag atcacaccgg gtgccactcc tgtcacacct gaggctacaa gtgacgctca
     6721 ccaaaattgg acaataggaa aaacattgcc tgctctgatg agtctccatt tttctgctgc
     6781 aacattcgta tggtagggtc agaatttggc atcaacaaca tgaaagcatg gatccatcct
     6841 gccttgtatc aatgattcag gctgaagatg gtggtgtaat ggtgtggggg atattttctt
     6901 gtgacacttt gggcccatta gtatcaactg agcatcatgt aaatgccaca gcctacctga
     6961 gtattgtcgc tgaccatgtc catctcttta agaccacagt gtacccatct cctgatggct
     7021 acttccagca ggatatcgta ccatgtcata aagcgtgaaa catctcagac tggtttctta
     7081 aacatgacaa tgaggtcact gtactcaaat ggcctccaca gtcaccaggc ctcaatccaa
     7141 tagagcacct ttggaatgtg gtggaatggg agatttgcat catggatgtg catccaacaa
     7201 atctgcagca actgtgtgat gccatcatgt caatatgacc aaaatctctg aggaatattt
     7261 ccagttcaat ccatgccacg aaggattaag gcagctctaa aggcataagc gggtcctaac
     7321 ctggtactag taaggtgtac ctaataaagt ggctagtgtg tgtaagttac tcaaaaatat
     7381 tagttttaaa acataaaagc agtctgtatt caaaatagtt acctcagctc ttcggtttta
     7441 cagtgtttac tgacagaatc aagcagcttt acatgagtga caggggtgtt tctctgaact
     7501 gcacactgct gtgttttttt gaagtacgac tgcaaaagaa atgtgacagt ctgtgactct
     7561 acagtgaccc gtctgctaaa gtgaatttga cacatgctga aaaaaaagcc atgactagaa
     7621 cggaccacac atttgtttat tcagctcggc tctgatgtca acaatgaaat atctttcctt
     7681 taagaaagcc atttcaaaag agacgtaaag acattgttgc tcttattcgt tttgaatact
     7741 gttggcaggt tatacactgg ctaagttaat ataaaaatgt tagattgtaa ttgtaattta
     7801 tatttaataa taataataat aataatacat tttatttata ggcgcctttc tagcatctca
     7861 aggacaccgt acaatcaaca agagcctaaa acatcaagag aaataataaa atatacacaa
     7921 caatagtgca gataaaatga agtattaaaa gccatcttaa ataagtgggt tttgagtttt
     7981 gatttgaata gtgtgaggga gtcaatgttt ctgatggcag gtggtaatga gttccaaagc
     8041 cgcggagcag agcggctaaa tgctctgttg cccatagttg aaagacgaga agagggaaca
     8101 gacaagtgga gggaggaaga agatcgcaga gtgcgagaag gaacagagat gtggatgagg
     8161 gcagacaaat atggaggggc gagattgcgg atggctttga atgttaacat caagatttta
     8221 aagtcaattc gaaaatgaac aggtcattta aattggtata cgttacactg taaaaaaaaa
     8281 aaaagccaca caattcaggt catcacaaca caactcaatt agttaacaaa tttaagtgga
     8341 ttgaacataa aacaacccca acaaaaaaat ctcaagaatc gtgttgattc agctctttta
     8401 aaaaatagtt tgaacaagct gcaaaaatct tttattttgt gtaaagcaat tactatcaga
     8461 attacaaaaa atcattcggg caataaattg actcaggttt ctttccaatg aaagatgtgt
     8521 actctcaata actcctctca aaacactctt tctttcactt ttctttcaca aacaacggag
     8581 caggggagaa atagcgaata tatctaaaca tatcaacacg tgtaaactgt acataaattt
     8641 gtgcgttatt aataaattat attaaataaa atttgagctc tttcgatatc tttagtttca
     8701 agcttaaaca tgtcctagat aaaacctact gatttgaagg gtagcgtata aagatttaaa
     8761 aagacaataa attatttagc actttcatcc ttaaataggg tcttttaata cgtcgtcaaa
     8821 tgaattatag aatttttttt ttggtgttta aatgaatgtt acactgagtg acattttaga
     8881 ggcatcatgg taaaattgca tgatagtcat tttttttatt actgattaaa aataaaatac
     8941 aagtactatt aataatataa aaaaactact aataactaaa aaataatata tgaataaaat
     9001 taactgagca cattcgaagg aacaaaatat gttttttaaa tgataatatt gatattattt
     9061 aaatattaat attgttttga tgcttaaaac ccattttcat gggttttcat gggtccgttt
     9121 tactttattt taagcatttt taacaagaac aaacattggc tcatacttta cacgaacaaa
     9181 agacaatata aaagaaaaca acaaagaaaa gtgacaaaaa acacacacac acacacacac
     9241 acacacacat catagtcaca aaacatgcaa ttaaaactag agagggtgta tagtttaaac
     9301 ctcagagggg tcagtggatt gctcttgaga tatagatttg tcaaataaat caagaaattg
     9361 tcctcaaata gccaaaattt ttctcgtgga gctagtcctt aaaattctga gtctctctat
     9421 ctgaatgaca gacatcatat cagaaatcca gttctggaat gagggggggg gtgttgtaaa
     9481 gatatcgtaa aacacgtgat tttttggaca gagttcgaaa ttgggaattt tggacttcac
     9541 aaaatgcctt ggtttacaat catttaatac catttatatt gtttataata tattaataat
     9601 ataatagtaa tatatcatta aacacgctta aaaaaacttc tgcaatttgt cttgaaggtt
     9661 tttcattctt ctcttctgaa ctcatcaacc aacaagataa cataagtgaa atcaagttac
     9721 agctggttct ttagatcatt ctcattttaa agatgtgaca ttattccaat agtatctgga
     9781 tgcagccttt atgatgcaag ctgagattgc atcactcagc gatgcaatgt caggcacaat
     9841 gcactcaaat ggaacatgtg acgtcactgt gatgggtggg gttcggtgag cccattaaaa
     9901 agcattggat gcagctcaga ctgcatctgc accaggtctg catccagacc catctctgtt
     9961 attctgcagt cttagttatc tatagtggtt agcaacagtt acttcacagc aagaagattg
    10021 ctggttcaag ccctggctgg gttatttacg tgtttctgtg tggagtttgc atgttctcct
    10081 tgtgttggtg tgggtttcct ccggtttcca tcacagcccg aagacatgcg ctattgggga
    10141 attaaataaa ctaaaatggc tgtagtgttt gtgtgtaaat gcgagagtgt ataggtgttt
    10201 cccagaactg gattgcagct ggaagggcat ccagtgcata aaacatatgc tggaatagtt
    10261 ggcggttcat tccgctgtag tgaccgctga taaacaaagg gactaagccg aagaaaaact
    10321 attggatgaa cgtgatacat gtaaaagagt gaagttggtg taattgtatc tggctaagat
    10381 gtttgtagtc tgttgtcctc ataaccacct tctgtatctc agtctgaacg tatgtagtgt
    10441 caagatcagc gtgacccaat tctcagaaaa aataatctta ttaaaataaa cagcgtaatc
    10501 acagattatc tttcgctcac ctttctgcat cagtctcgta aacacagtct aacgtttcgt
    10561 attcacttta acaatataca tatttccgca caaaatcagc aattcagcat ttttactttt
    10621 caaatgcgta catttcaaac atatattatt acagtttact tcttagtaga atatggtaga
    10681 ttgaaaatcc aatagtttaa ttaagtcaac aacaatgtgt tttgtctttt gtaatgatct
    10741 tggtttgtgt gtaaaaaaat gtaatttgaa tgtgactatc cacaaggaat ttacaacatt
    10801 gtaaaaagaa attgctacat ttattaaccg aaaaatattt atgaataatt ttccacaatg
    10861 ttgacatcag cagagaatga aatgctgttt tcgagacaca gccactagaa aatcaacgga
    10921 tgtttaccaa aacaaaaatg tcttatagtt tgaaaaaata aaaagcatat gaagtattaa
    10981 gaaaagtgaa cagtcaattt tgattttacc gtttttttaa acactcaagt aaaatgtttt
    11041 tatttcaaat aatttttata ataaaaacta aatatttata aagtttcaca aagtaattta
    11101 ttcattcatt ttccttcggc ttagtccctt tattaatccg gggtcgccac agcggaatga
    11161 tccgccaacc catctctggg aaacatcaac acacacacac acacacacac tacggacaat
    11221 ttagcctcgg ctcagttggc gtttctgtgt ggagtttgca tgttctccct gccttcgcgt
    11281 gggtttcccc ctggtgctcc ggtttccccc acagtccaaa gacatgcggt gcaggtgaat
    11341 tgggtaggct aaattgtccg tagagtatga gcgtgtgtgt gaatgtgtgt gtggatgttt
    11401 cccagtgatg ggttgcggct ggaagggcat ccgttgtgta aaaacttgct ggataagttg
    11461 gcggttcatt ccgctgtggc gaccccggat taataaaggg actaagccga caagaaaatg
    11521 aatgaatgaa tgaatgaata tctgattttg tcattcagac catttttcaa ccaaaccttt
    11581 atttgtccca caactactca tgtttaagaa acaatttgat attttagaaa tttatgacag
    11641 catatagtga cattatacag tatatagtta aagtttaaat tattagccct ttgtgaattt
    11701 ttctatattt ttgaaaatat ttttcacatt ttttttcaca gtatttccaa taatattttt
    11761 ttttcttcag gagaaagtct tatttgtttt atttcagcta gaataaaagc agtttatata
    11821 taaagcattt taaggtcaat tctatttatt ttggattgtc tacaggacaa actattgtta
    11881 tacaatgact tgccttatta atctaagtta ggccattaaa ttgcacttta agctgaatac
    11941 tagtatcttg aaaaatatct agtcaaatat tatgtcctgt catcatggca aaaataaaag
    12001 aaataagtta ttaaaaccaa cacaggctca ttctgaaaac atagccctac tgtatataca
    12061 tttctggaga ttgcgaatta tgtagccaga agtaagtatg gctatatttc gtctttaaaa
    12121 tgaacactag ggggcgatat gatgccgttc cttttctcgc ttaccagctg accacttacc
    12181 tcagtataga cggctttccc gctgttacca gtttgtccaa tagctcgcgc atacatcgca
    12241 gtcttgaaac acagagaagg gttgactgcg ccgacggggt tcaagtccag tgaagaacgc
    12301 ttccagaaag caggtaagac ataaacaaaa gccaaaaata aaataaacaa gtaaataaca
    12361 ttttgataat gtaagtaaaa gctgaaaaca ttgtaaaaat caggcgaggg cttttctttt
    12421 tctggattgc ttttcaaaac actgtcggtt gggcttagga aagtgggtcg gcaagtcaat
    12481 ttgagatctc aaaaggcgta cacagcggcc tctggtggat tcgcataaac aaaaaactgc
    12541 aaaaaaacat atctcctggg atgttttggt gctctccaga aatgtatata ggggtacgtt
    12601 ttcagaatga gcctgggttg attcaaacta taatgtttag aaatgtataa aaatgaaaat
    12661 ttaactggag ggctaataat tctgaactgt atgtatacat tttttttatt gcaaaaaaac
    12721 atcctgtaaa ataacacatt ttaaaaatac accaaaaaca caaagttctg caacctaaaa
    12781 tcctcattcc accaacaata tgtttcaaca ttttcagcat tcgccactaa tgcaggcaca
    12841 ttcagcatct tttaaaccca gttcctccat gcatctattt ttaccagccg tcatgcacag
    12901 aggagcattg cagcaccctg tgtttgttgt ctcccctcct cttcccgggt cccttgttcc
    12961 cgcttattaa tcagtcgcct tgacctactc atcctcctgt ttctctcccc tctaattctc
    13021 agcatcttcc tctgactcat cttttcatcc cctccccctt ctgtttctcc tctcatctcg
    13081 ctctcattct gctttcctcc caccctcgct ctgttttcct ctctcttccg cagtttcaca
    13141 cttccaggct tgcctctcgc aaggctacac gcactgacgg caggtaagaa gcctttttat
    13201 ttgacatcta tttaggcagc tcatttccat catctgccat ggggtcattg aggtcattat
    13261 gctaattatt ctgtatacgg acttgatttg tgaatgttcc agttagaaaa ggaagacatc
    13321 gactcgtgcg tgaggtgtca cttcttatgt tgggatgctg gatttgattt tgggtggcgt
    13381 tttttttttt tttttggtgt ccgctggtag gctaaggggt cgggcagatg gatatcgcac
    13441 tatgtgtttg caacactctt tctgtattgc ctctcagagt gtgtgtgtgt gtgtgtgttg
    13501 tcagtgtgtg tccaatctgt tctaattcgt gatgtaagag tgggtcaatt tacagaattg
    13561 ctcatgatac atacagtttt gactaattta accaaagttt aatcgaaata ttacattttt
    13621 tacccctaaa tgaacacaaa tatgattaaa aatgcaacta tttatacagg aaaagataag
    13681 aaacaaggaa aaaatcgcga gtcgtacgga gttatttgta tattagtcac gtgacgcgcg
    13741 cgaaaaaaaa cacagaaacg aatcgctcgt gaacaaccca ctacatacta ctcgcttcga
    13801 tgctccattt cagaaagctc ttcttttcgc caaaggcatg ttaaaatatt tatcttgttc
    13861 ttgccccatc acgccacttt ctgcgctata tgcaccccac ccgtccgccc cctcctcccc
    13921 ttatatctgc tgcagtacca cacacactct ctctcacaca cacacattgg caaattagcc
    13981 tcatccggat ttagatgctc gatcataacc tgagcacctg cgggcaaaca ttccactgtg
    14041 tcatcccgaa taagggccct cctcctcggc cgctttcggt gctggtggtc gcccgttgca
    14101 ttaacaaggt gacacgtgcg cgcgcccatg tcggttgtag catatttagg atgcatttcg
    14161 catgttctgc ttttccattg gctgccagct caggcatctc catgtcgatg cttgagcagt
    14221 ttctcccact gctctacaaa agacgggact gaatgttcca gaaaaacaca gccgttttaa
    14281 aaaggcacca tcagattgat tgacatctcc tgacgcgttt gcagagatgc tgaagtccaa
    14341 aaaaagatgg cggtcacttc gtgtatgttt gtggggttat tttgtagaat taaagtgcat
    14401 attttaaata ttatataata ttcacagtct agccattttc cccatactat ttttccagtc
    14461 acacgcaatt ctttataaac cgtttgctat gactttgcct caataaactg ctaatttgct
    14521 gcttattaat agctagtagt tggttttaag tactaggtag gattagggat atgtaaaaaa
    14581 gatcaacaca gaacagaata tgtgctttat aagtactaat aaacaattat taaaccacta
    14641 atagtgagaa ttggtgctta aactacttat tttttattga atagcaaatg gtaataataa
    14701 ttcctgtgaa ttgtctagtc aagctgtcaa aatcccgaag cctaagggtg tgttcacact
    14761 gcaagtttgg tttaattaaa gtttggtaca caccaaggtg atggtcacct gtagtcattg
    14821 tcctaatgac tacgttacat ggacatcagt cattgatagg gctgggtgat tttaaaacga
    14881 aaatcttgat taattgaacg tcttaaatcg attacaatta atgaacgatt attttattta
    14941 tttcctaatt gtattttttg tcttcatagt tttatatgcc atcttaaaga atctctggcg
    15001 cagcttccaa tcatcgtcag tgtagtgcaa agtcaagaat gagtccatcg tcctactggt
    15061 ccagagatca gatgtggctg caaagtggct gcagaaatca gccacagcct ttaacagagt
    15121 ggggtcacgt gacacattaa gttatattgt tatattgaaa agttatattg attataggtt
    15181 ctgaatgttg acttcgattt cttttcgatt aatcgcccag tgatcaaatg atttgcctta
    15241 atcgaaaaaa atataatgat gatgtttaca tgagttgctt tttgaatgtt ccttttatac
    15301 atgttatata tcattataca gcaattaatg tcattgcctc agcccgctat ccatgtttcc
    15361 tctggagttt catgtaattt cgggtgtttt gtttttaatt tgttgacttt aactgcactt
    15421 tggcactttc actttcatct ggaacatttc atgtatgtcc gccctgacaa acaagatatt
    15481 agatgtgtgg aactgctgga agagtgtagt tttttttttt tttttgaaac ctttattgta
    15541 taataagaca gtagagagta ttgacaggaa agcatgggga gcagagagag gggaaggccg
    15601 tgaggcagga ttcaaactca ggtcgccgtg agcaccggtg tgtcgaccca ctaactaacc
    15661 actgcaccac tggcgccgac gaaaagtgta gttttaaagg aatttgatac catatgccga
    15721 atagggaaac aacctctgca tttcatgatg tgtgtgtctg tgttccttaa ctgcctcggt
    15781 aggtgcagag aagtgtcaaa cagttgtctg tgtatagaca aaatgcggtg atagtttgat
    15841 tgtgttgttt acatgtttgt actgcacttc aataacgaca gcgagagcat ctaagttcac
    15901 tatggcgcac ctgacaacaa cgatctggcc ctgagagcac caaaatttgc gacattactc
    15961 atatttaaag gggccgttgc agcatatcag caaatcacca acattcccgc atacaaatgt
    16021 gctttctcgt gtgaaaatgt tgcgcgcctt ttgtcaaatt catttaatac agaagatcca
    16081 gttgtttgca tgtgctgtgg ctttgctctt atccaatatg ttaatatgtc tgaaatattc
    16141 tgaagcagca atattgtttt gtactctcat tagttagcat aagattcccg tttaaaaaca
    16201 aacatatata acatttaatg cacatcagaa actcgccagt aaattttttt tatttaagac
    16261 cctgtcagaa aacattgtga acaattaaaa atccggcaac agcaatatta aaaacccttg
    16321 ggaacaacag tggtcagaac caaagttcac agggctgtgt tcagtggact tctctcaagt
    16381 ggtggacctt tacaatctgc catactatgg ctacaacagg cacactgata ttacagacgt
    16441 ttcatttttc gtccatcaca atgtatcagc agaagagtca agcttcaaat aatacaaact
    16501 ctttcgccat ttttgagtga catgctaatg gtctaatcgg attcaatgat gtatgctaag
    16561 ctaagctaaa agtgctcccg ccaaacctgt taaatgtctg aatggattaa aaaattataa
    16621 aactgaactg tttaacttta ggggagctgt aaaggaactt attttttaaa aagtggagtg
    16681 ttactttaaa ataaatgcca taaacagtgt ctaaacaaca cactgacatt gagaaaagta
    16741 gaggtttagc ttatcgtttg tctgtttttg ctcttgtctt taagg
//
