LOCUS Exported 8877 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression vector for xCas9(3.7)-BE4. ACCESSION . VERSION . KEYWORDS xCas9(3.7)-BE4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8877) AUTHORS Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR TITLE Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. JOURNAL Nature. 2018 Feb 28. pii: nature26155. doi: 10.1038/nature26155. PUBMED 29512652 REFERENCE 2 (bases 1 to 8877) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8877 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 74..453 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 454..657 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 608..628 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 699..718 /label=T7 /note="T7 promoter, forward primer" promoter 699..717 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 731..1417 /codon_start=1 /product="cytidine deaminase (C to U editing enzyme) from rat" /label=APOBEC-1 /note="can use ssDNA as a substrate (Komor et al., 2016)" /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLK" CDS 1514..5614 /codon_start=1 /product="Streptococcus pyogenes Cas9 mutant with broad PAM compatibility (Hu et al., 2018)" /label=xCas9-3.7 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDTKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKLYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGIIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEKVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGDQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFIQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGVLQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5645..5893 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 5924..6172 /codon_start=1 /product="uracil-DNA glycosylase inhibitor from a Bacillus subtilis bacteriophage (Mol et al., 1995)" /label=UGI /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML" CDS 6185..6205 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6214..6231 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(6254..6271) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6260..6484 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(6555..6571) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6555..6571) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6568..6590) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6579..6595 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6603..6633) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6648..6669 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6786..6803) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6957..7545) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7037..7056) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7716..8576) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8339..8358 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8577..8681) /gene="bla" /label=AmpR promoter primer_bind complement(8760..8779) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc 61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca 121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt 661 ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag 721 agccgccacc atgagctcag agactggccc agtggctgtg gaccccacat tgagacggcg 781 gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca aggagacctg 841 cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata catcacagaa 901 cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa gatatttctg 961 tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg gcgaatgtag 1021 tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta tttacatcgc 1081 aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt tgatctcttc 1141 aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga gaaactttgt 1201 gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt gggtacgact 1261 gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca acattctgag 1321 aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc attaccagcg 1381 actgccccca cacattctct gggccaccgg gttgaaatct ggtggttctt ctggtggttc 1441 tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt ctggtggttc 1501 ttctggtggt tctgacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg 1561 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa 1621 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga 1681 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa 1741 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt 1801 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc 1861 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca 1921 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc 1981 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga 2041 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga 2101 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc 2161 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct 2221 gtttggtaat cttatcgccc tgtccctcgg gctgaccccc aactttaaat ctaacttcga 2281 cctggccgaa gataccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa 2341 tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc 2401 agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca aagctccgct 2461 gagcgctagt atgatcaagc tctatgatga gcaccaccaa gacttgactt tgctgaaggc 2521 ccttgtcaga cagcaactgc ctgagaagta caaggaaatt ttcttcgatc agtctaaaaa 2581 tggctacgcc ggatacattg acggcggagc aagccaggag gaattttaca aatttattaa 2641 gcccatcttg gaaaaaatgg acggcaccga ggagctgctg gtaaagctta acagagaaga 2701 tctgttgcgc aaacagcgca ctttcgacaa tggaatcatc ccccaccaga ttcacctggg 2761 cgaactgcac gctatcctca ggcggcaaga ggatttctac ccctttttga aagataacag 2821 ggaaaagatt gagaaaatcc tcacatttcg gataccctac tatgtaggcc ccctcgcccg 2881 gggaaattcc agattcgcgt ggatgactcg caaatcagaa gagaccatca ctccctggaa 2941 cttcgagaaa gtcgtggata agggggcctc tgcccagtcc ttcatcgaaa ggatgactaa 3001 ctttgataaa aatctgccta acgaaaaggt gcttcctaaa cactctctgc tgtacgagta 3061 cttcacagtt tataacgagc tcaccaaggt caaatacgtc acagaaggga tgagaaagcc 3121 agcattcctg tctggagatc agaagaaagc tattgtggac ctcctcttca agacgaaccg 3181 gaaagttacc gtgaaacagc tcaaagaaga ctatttcaaa aagattgaat gtttcgactc 3241 tgttgaaatc agcggagtgg aggatcgctt caacgcatcc ctgggaacgt atcacgatct 3301 cctgaaaatc attaaagaca aggacttcct ggacaatgag gagaacgagg acattcttga 3361 ggacattgtc ctcaccctta cgttgtttga agatagggag atgattgaag aacgcttgaa 3421 aacttacgct catctcttcg acgacaaagt catgaagcag ctcaagaggc gccgatatac 3481 aggatggggg cggctgtcaa gaaaactgat caatgggatc cgagacaagc agagtggaaa 3541 gacaatcctg gattttctta agtccgatgg atttgccaac cggaacttca ttcagttgat 3601 ccatgatgac tctctcacct ttaaggagga catccagaaa gcacaagttt ctggccaggg 3661 ggacagtctt cacgagcaca tcgctaatct tgcaggtagc ccagctatca aaaagggaat 3721 actgcagacc gttaaggtcg tggatgaact cgtcaaagta atgggaaggc ataagcccga 3781 gaatatcgtt atcgagatgg cccgagagaa ccaaaccacc cagaagggac agaagaacag 3841 tagggaaagg atgaagagga ttgaagaggg tataaaagaa ctggggtccc aaatccttaa 3901 ggaacaccca gttgaaaaca cccagcttca gaatgagaag ctctacctgt actacctgca 3961 gaacggcagg gacatgtacg tggatcagga actggacatc aatcggctct ccgactacga 4021 cgtggatcat atcgtgcccc agtcttttct caaagatgat tctattgata ataaagtgtt 4081 gacaagatcc gataaaaaca gagggaagag tgataacgtc ccctcagaag aagttgtcaa 4141 gaaaatgaaa aattattggc ggcagctgct gaacgccaaa ctgatcacac aacggaagtt 4201 cgataatctg actaaggctg aacgaggtgg cctgtctgag ttggataaag ccggtttcat 4261 caaaaggcag cttgttgaga cacgccagat caccaagcac gtggcccaaa ttctcgattc 4321 acgcatgaac accaagtacg atgaaaatga caaactgatt cgagaggtga aagttattac 4381 tctgaagtct aagctggtct cagatttcag aaaggacttt cagttttata aggtgagaga 4441 gatcaacaat taccaccatg cgcatgatgc ctacctgaat gcagtggtag gcactgcact 4501 tatcaaaaaa tatcccaagc ttgaatctga atttgtttac ggagactata aagtgtacga 4561 tgttaggaaa atgatcgcaa agtctgagca ggaaataggc aaggccaccg ctaagtactt 4621 cttttacagc aatattatga attttttcaa gaccgagatt acactggcca atggagagat 4681 tcggaagcga ccacttatcg aaacaaacgg agaaacagga gaaatcgtgt gggacaaggg 4741 tagggatttc gcgacagtcc ggaaggtcct gtccatgccg caggtgaaca tcgttaaaaa 4801 gaccgaagta cagaccggag gcttctccaa ggaaagtatc ctcccgaaaa ggaacagcga 4861 caagctgatc gcacgcaaaa aagattggga ccccaagaaa tacggcggat tcgattctcc 4921 tacagtcgct tacagtgtac tggttgtggc taaagtggag aaagggaagt ctaaaaaact 4981 caaaagcgtc aaggaactgc tgggcatcac aatcatggag cgatcaagct tcgaaaaaaa 5041 ccccatcgac tttctcgagg cgaaaggata taaagaggtc aaaaaagacc tcatcattaa 5101 gcttcccaag tactctctct ttgagcttga aaacggccgg aaacgaatgc tcgctagtgc 5161 gggcgtgctg cagaaaggta acgagctggc actgccctct aaatacgtta atttcttgta 5221 tctggccagc cactatgaaa agctcaaagg gtctcccgaa gataatgagc agaagcagct 5281 gttcgtggaa caacacaaac actaccttga tgagatcatc gagcaaataa gcgaattctc 5341 caaaagagtg atcctcgccg acgctaacct cgataaggtg ctttctgctt acaataagca 5401 cagggataag cccatcaggg agcaggcaga aaacattatc cacttgttta ctctgaccaa 5461 cttgggcgcg cctgcagcct tcaagtactt cgacactacc atagacagaa agcggtacac 5521 ctctacaaag gaggtcctgg acgccacact gattcatcag tcaattacgg ggctctatga 5581 aacaagaatc gacctctctc agctcggtgg agactctggt ggttctggag gatctggtgg 5641 ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg ttatccagga 5701 atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc cggaaagcga 5761 tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc ttctgactag 5821 cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg gtgagaacaa 5881 gattaagatg ctctctggtg gttctggagg atctggtggt tctactaatc tgtcagatat 5941 tattgaaaag gagaccggta agcaactggt tatccaggaa tccatcctca tgctcccaga 6001 ggaggtggaa gaagtcattg ggaacaagcc ggaaagcgat atactcgtgc acaccgccta 6061 cgacgagagc accgacgaga atgtcatgct tctgactagc gacgcccctg aatacaagcc 6121 ttgggctctg gtcatacagg atagcaacgg tgagaacaag attaagatgc tctctggtgg 6181 ttctcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa 6241 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 6301 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 6361 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 6421 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 6481 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc 6541 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 6601 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa 6661 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 6721 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 6781 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 6841 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 6901 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 6961 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 7021 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 7081 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 7141 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7201 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7261 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7321 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7381 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 7441 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 7501 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 7561 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7621 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7681 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7741 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7801 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7861 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7921 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7981 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 8041 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 8101 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 8161 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 8221 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 8281 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 8341 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 8401 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 8461 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 8521 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 8581 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8641 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8701 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt 8761 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag 8821 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgac //