LOCUS Exported 14289 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion. ACCESSION . VERSION . KEYWORDS GFP-UBR5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14289) AUTHORS Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B, Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C TITLE TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes. JOURNAL Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. Epub 2012 Aug 9. PUBMED 22884692 REFERENCE 2 (bases 1 to 14289) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14289 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" CDS 709..1425 /codon_start=1 /product="Emerald GFP" /label=EmGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(754..775) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1015..1034) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1362..1383 /label=EGFP-C /note="EGFP, forward primer" protein_bind 1441..1465 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(9880..9904) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 9912..9953 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" primer_bind complement(9993..10012) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 10037..10085 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 10287..10715 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(10374..10393) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 10584..10605 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10724..10744) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 10729..11058 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 10909..11044 /label=SV40 ori /note="SV40 origin of replication" primer_bind 10971..10990 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 11106..11153 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 11172..11570 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" polyA_signal 11728..11849 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(11765..11784) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11819..11838 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(11898..11914) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11898..11914) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(11911..11933) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 11922..11938 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(11946..11976) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 11991..12012 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(12129..12146) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(12300..12888) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(12380..12399) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13059..13919) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 13682..13701 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(13920..14024) /gene="bla" /label=AmpR promoter primer_bind complement(14099..14118) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg gactctagag gatccctacc ggtgatatcc tcgagaccat ggtgagcaag 721 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 781 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 841 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 901 ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc 961 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 1021 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 1081 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 1141 aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg 1201 aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 1261 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 1321 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 1381 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggctc gagcccatca 1441 acaagtttgt acaaaaaagc aggcttccgg accatgacgt ccatccattt cgtggttcac 1501 ccgctgccgg gcaccgagga ccagctcaat gacaggttac gagaagtttc tgagaagctg 1561 aacaaatata atttaaacag ccacccccct ttgaatgtat tggaacaggc tactattaaa 1621 cagtgtgtgg tgggaccaaa tcatgctgcc tttcttcttg aggatggtag agtttgcagg 1681 attggttttt cagtacagcc agacagattg gaattgggta aacctgataa taatgatggg 1741 tcaaagttga acagcaactc gggggcaggg aggacgtcaa ggcctggtag gacaagcgac 1801 tctccatggt ttctctcagg ttctgagact ctaggcaggc tggcaggcaa caccttagga 1861 agccgctgga gttctggagt gggtggaagt ggtggaggat cctctggtag gtcatcagct 1921 ggagctcgag attcccgccg gcagactcga gttattcgga caggacggga tcgagggtct 1981 gggcttttgg gcagtcagcc ccagccagtt attccagcat ctgtcattcc agaggagctg 2041 atttcacagg cccaagttgt tttacaaggc aaatccagaa gtgtcattat tcgagaactt 2101 cagagaacaa atcttgatgt gaaccttgct gtaaataatt tacttagccg ggatgatgaa 2161 gatggagatg atggggatga tacagccagc gaatcttatt tgcctggaga ggatcttatg 2221 tctctccttg atgccgacat tcattctgcc cacccaagtg tcattattga tgcagatgcc 2281 atgttttctg aagacattag ctattttggt tacccttctt ttcgtcgttc atcactttcc 2341 aggctaggct catctcgagt tctccttctt cccttagaga gagactctga gctgttgcgt 2401 gaacgtgaat ccgttttacg tttacgtgaa cgaaggtggc ttgatggagc ctcatttgat 2461 aatgaaaggg gttctaccag caaggaagga gagccaaact tggataagaa gaatacacct 2521 gttcaaagtc cagtatctct aggagaagat ttgcagtggt ggcctgataa ggatggaaca 2581 aaattcatct gtattggggc tctgtattct gaacttctgg ctgtcagcag taaaggagaa 2641 ctttatcagt ggaaatggag tgaatctgag ccttacagaa atgcccagaa tccttcatta 2701 catcatccac gagcaacatt tttggggtta accaatgaaa agatagtcct cctgtctgca 2761 aatagcataa gagcaactgt agctacagaa aataacaagg ttgctacatg ggtggatgaa 2821 actttaagtt ctgtggcttc taaattagag cacactgctc agacttactc tgaacttcaa 2881 ggagagcgga tagtttcttt acattgctgt gccctttaca cctgcgctca gctggaaaac 2941 agtttatatt ggtggggtgt agttcctttt agtcaaagga ggaaaatgtt agagaaagct 3001 agagcaaaaa ataaaaagcc taaatccagt gctggtattt cttcaatgcc gaacatcact 3061 gttggtaccc aggtatgctt gagaaataat cctctttatc atgctggagc agttgcattt 3121 tcaattagtg ctgggattcc taaagttggt gtcttaatgg agtcagtttg gaatatgaat 3181 gacagctgta gatttcaact tagatctcct gaaagcttga aaaacatgga aaaagctagc 3241 aaaactactg aagctaagcc tgaaagtaag caggagccag tgaaaacaga aatgggtcct 3301 ccaccatctc cagcatccac gtgtagtgat gcatcctcaa ttgccagcag tgcatcaatg 3361 ccatacaaac gacgacggtc aacccctgca ccaaaagaag aggaaaaggt gaatgaagag 3421 cagtggtctc ttcgggaagt ggtttttgtg gaagatgtca agaatgttcc tgttggcaag 3481 gtgctaaaag tagatggtgc ctatgttgct gtaaaatttc caggaacctc cagtaatact 3541 aactgtcaga acagctctgg tccagatgct gacccttctt ctctcctgca ggattgtagg 3601 ttacttagaa ttgatgaatt gcaggttgtc aaaactggtg gaacaccgaa ggttcccgac 3661 tgtttccaaa ggactcctaa aaagctttgt atacctgaaa aaacagaaat attagcagtg 3721 aatgtagatt ccaaaggtgt tcatgctgtt ctgaagactg gaaattgggt acgatactgt 3781 atctttgatc ttgctacagg aaaagcagaa caggaaaata attttcctac aagcagcatt 3841 gctttccttg gtcagaatga gaggaatgta gccattttca ctgctggaca ggaatctccc 3901 attattcttc gagatggaaa tggtaccatc tacccaatgg ccaaagattg catgggagga 3961 ataagggatc ccgattggct ggatcttcca cctattagta gtcttggaat gggtgtgcat 4021 tctttaataa atcttcctgc caattcaaca atcaaaaaga aagctgctgt tatcatcatg 4081 gctgtagaga aacaaacctt aatgcaacac attctgcgct gtgactatga ggcctgtcga 4141 caatatctaa tgaatcttga gcaagcggtt gttttagagc agaatctaca gatgctgcag 4201 acattcatca gccacagatg tgatggaaat cgaaatattt tgcatgcttg tgtatcagtt 4261 tgctttccaa ccagcaataa agaaactaaa gaagaagagg aagcggagcg ttctgaaaga 4321 aatacatttg cagaaaggct ttctgctgtt gaggccattg caaatgcaat atcagttgtt 4381 tcaagtaatg gcccaggtaa tcgggctgga tcatcaagta gccgaagttt gagattacgg 4441 gaaatgatga gacgttcgtt gagagcagct ggtttgggta gacatgaagc tggagcttca 4501 tccagtgacc accaggatcc agtttcaccc cccatagctc cccctagttg ggttcctgac 4561 cctcctgcga tggatcctga tggtgacatt gattttatcc tggcccccgc tgtgggatct 4621 cttaccacag cagcaaccgg tactggtcaa ggaccaagca cctccactat tccaggtcct 4681 tccacagagc catctgtagt agaatccaag gatcgaaagg cgaatgctca ttttatattg 4741 aaattgttat gtgacagtgt ggttctccag ccctatctac gagaacttct ttctgccaag 4801 gatgcaagag ggatgacccc atttatgtca gctgtaagtg gccgagctta tcctgctgca 4861 attaccatct tagaaactgc tcagaaaatt gcaaaagctg aaatatcctc aagtgaaaaa 4921 gaggaagatg tattcatggg aatggtttgc ccatcaggta ccaaccctga tgactctcct 4981 ttatatgttt tatgttgtaa tgacacttgc agttttacat ggactggagc agagcacatt 5041 aaccaggata tttttgagtg tcgaacttgt ggcttgctgg agtcactgtg ttgttgtacg 5101 gaatgtgcaa gggtttgtca taaaggtcat gattgcaaac tcaaacggac atcaccaaca 5161 gcctactgtg attgttggga gaaatgtaaa tgtaaaactc ttattgctgg acagaaatct 5221 gctcgtcttg atctacttta tcgcctgctc actgctacta atctggttac tctgccaaac 5281 agcaggggag agcacctctt actattctta gtacagacag tcgcaaggca gacggtggag 5341 cattgtcaat acaggccacc tcgaatcagg gaagatcgta accgaaaaac agccagtcct 5401 gaagattcag atatgccaga tcatgatttg gagcctccaa gatttgccca gcttgcattg 5461 gagcgtgttc tacaggactg gaatgccttg aaatctatga ttatgtttgg gtcgcaggag 5521 aataaagacc ctcttagtgc cagcagtaga ataggccatc ttttgccaga agagcaagta 5581 tacctcaatc agcaaagtgg cacaattcgg ctggactgtt tcactcattg ccttatagtt 5641 aagtgtacag cagatatttt gcttttagat actctactag gtacactagt gaaagaactc 5701 caaaacaaat atacacctgg acgtagagaa gaagctattg ctgtgacaat gaggtttcta 5761 cgttcagtgg caagagtttt tgttattctg agtgtggaaa tggcttcatc caaaaagaaa 5821 aacaacttta ttccacagcc aattggaaaa tgcaagcgtg tattccaagc attgctacct 5881 tacgctgtgg aagaattgtg caacgtagca gagtcactga ttgttcctgt cagaatgggg 5941 attgctcgtc caactgcacc atttaccctg gctagtacta gcatagatgc catgcagggc 6001 agtgaagaat tattttcagt ggaaccacta ccaccacgac catcatctga tcagtctagc 6061 agctccagtc agtctcagtc atcctacatc atcaggaatc cacagcagag gcgcatcagc 6121 cagtcacagc ccgttcgggg cagagatgaa gaacaggatg atattgtttc agcagatgtg 6181 gaagaggttg aggtggtgga gggtgtggct ggagaagagg atcatcatga tgaacaggaa 6241 gaacacgggg aagaaaatgc tgaggcagag ggacaacatg atgagcatga tgaagacggg 6301 agtgatatgg agctggactt gttagcagca gctgaaacag aaagtgatag tgaaagtaac 6361 cacagcaacc aagataatgc tagtgggcgc agaagcgttg tcactgcagc aactgctggt 6421 tcagaagcag gagcaagcag tgttcctgcc ttcttttctg aagatgattc tcaatcgaat 6481 gactcaagtg attctgatag cagtagtagt cagagtgacg acatagaaca ggagaccttt 6541 atgcttgatg agccattaga aagaaccaca aatagctccc atgccaatgg tgctgcccaa 6601 gctccccgtt caatgcagtg ggctgtccgc aacacccagc atcagcgagc agccagtaca 6661 gccccttcca gtacatctac accagcagca agttcagcgg gtttgattta tattgatcct 6721 tcaaacttac gccggagtgg taccatcagt acaagtgctg cagctgcagc agctgctttg 6781 gaagctagca acgccagcag ttacctaaca tctgcaagca gtttagccag ggcttacagc 6841 attgtcatta gacaaatctc ggacttgatg ggccttattc ctaagtataa tcacctagta 6901 tactctcaga ttccagcagc tgtgaaattg acttaccaag atgcagtaaa cttacagaac 6961 tatgtagaag aaaagcttat tcccacttgg aactggatgg tcagtattat ggattctact 7021 gaagctcaat tacgttatgg ttctgcatta gcatctgctg gtgatcctgg acatccaaat 7081 catcctcttc acgcttctca gaattcagcg agaagagaga ggatgactgc gcgagaagaa 7141 gctagcttac gaacacttga aggcagacga cgtgccacct tgcttagcgc ccgtcaagga 7201 atgatgtctg cacgaggaga cttcctaaat tatgctctgt ctctaatgcg gtctcataat 7261 gatgagcatt ctgatgttct tccagttttg gatgtttgct cattgaagca tgtggcatat 7321 gtttttcaag cacttatata ctggattaag gcaatgaatc agcagacaac attggataca 7381 cctcaactag aacgcaaaag gacgcgagaa ctcttggaac tgggtattga taatgaagat 7441 tcagaacatg aaaatgatga tgacaccaat caaagtgcta ctttgaatga taaggatgat 7501 gactctcttc ctgcagaaac tggccaaaac catccatttt tccgacgttc agactccatg 7561 acattccttg ggtgtatacc cccaaatcca tttgaagtgc ctctggctga agccatcccc 7621 ttggctgatc agccacatct gttgcagcca aatgctagaa aggaggatct ttttggccgt 7681 ccaagtcagg gtctttattc ttcatctgcc agtagtggga aatgtttaat ggaggttaca 7741 gtggatagaa actgcctaga ggttcttcca acaaaaatgt cttatgctgc caatctgaaa 7801 aatgtaatga acatgcaaaa ccggcaaaaa aaagaagggg aagaacagcc cgtgctgcca 7861 gaagaaactg agagttcaaa accagggcca tctgctcatg atcttgctgc acaattaaaa 7921 agtagcttac tagcagaaat aggacttact gaaagtgaag ggccacctct cacatctttc 7981 aggccacagt gtagctttat gggaatggtt atttcccatg atatgctgct aggacgttgg 8041 cgcctttctt tagaactgtt cggcagggta ttcatggaag atgttggagc agaacctgga 8101 tcaatcctaa ctgaattggg tggttttgag gtaaaagaat caaaattccg cagagaaatg 8161 gaaaaactga gaaaccagca gtcaagagat ttgtcactag aggttgatcg ggatcgagat 8221 cttctcattc agcagactat gaggcagctt aacaatcact ttggtcgaag atgtgctact 8281 acaccaatgg ctgtacacag agtaaaagtc acatttaagg atgagccagg agagggcagt 8341 ggtgtagcac gaagttttta tacagccatt gcacaagcat ttttatcaaa tgaaaaattg 8401 ccaaatctag agtgtatcca aaatgccaac aaaggcaccc acacaagttt aatgcagaga 8461 ttaaggaacc gaggagagag agaccgggaa agggagagag aaagggaaat gaggaggagt 8521 agtggtttgc gagcaggttc tcggagggac cgggatagag actttagaag acagctttcc 8581 atcgacacta ggccctttag accagcctct gaagggaatc ctagcgatga tcctgagcct 8641 ttgccagcac atcggcaggc acttggagag aggctttatc ctcgtgtaca agcaatgcaa 8701 ccagcatttg caagtaaaat cactggcatg ttgttggaat tatccccagc tcagctgctt 8761 ctccttctag caagtgagga ttctctgaga gcaagagtgg atgaggccat ggaactcatt 8821 attgcacatg gacgggaaaa tggagctgat agtatcctgg atcttggatt agtagactcc 8881 tcagaaaagg tacagcagga aaaccgaaag cgccatggct ctagtcgaag tgtagtagat 8941 atggatttag atgatacaga tgatggtgat gacaatgccc ctttgtttta ccaacctggg 9001 aaaagaggat tttatactcc aaggcctggc aagaacacag aagcaaggtt gaattgtttc 9061 agaaacattg gcaggattct tggactatgt ctgttacaga atgaactctg tcctatcaca 9121 ttgaatagac atgtaattaa agtattgctt ggtagaaaag tcaattggca tgattttgct 9181 ttttttgatc ctgtaatgta tgagagtttg cggcaactaa tcctcgcgtc tcagagttca 9241 gatgctgatg ctgttttctc agcaatggat ttggcatttg caattgacct gtgtaaagaa 9301 gaaggtggag gacaggttga actcattcct aatggtgtaa atataccagt cactccacag 9361 aatgtatatg agtatgtgcg gaaatacgca gaacacagaa tgttggtagt tgcagaacag 9421 cccttacatg caatgaggaa aggtctacta gatgtgcttc caaaaaattc attagaagat 9481 ttaacggcag aagattttag gcttttggta aatggctgcg gtgaagtcaa tgtgcaaatg 9541 ctgatcagtt ttacctcttt caatgatgaa tcaggagaaa atgctgagaa gcttctgcag 9601 ttcaagcgtt ggttctggtc aatagtagag aagatgagca tgacagaacg acaagatctt 9661 gtttactttt ggacatcaag cccatcactg ccagccagtg aagaaggatt ccagcctatg 9721 ccctcaatca caataagacc accagatgac caacatcttc ctactgcaaa tacttgcatt 9781 tctcgacttt acgtcccact ctattcctct aaacagattc tcaaacagaa attgttactc 9841 gccattaaga ccaagaattt tggttttgtg tagccgcgga cccagctttc ttgtacaaag 9901 tggttgataa cgggaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg 9961 gttagtaatg agtttaaacg ggggaggcta actgaaacac ggaaggagac aataccggaa 10021 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt 10081 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc 10141 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg 10201 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cagatctgcg 10261 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 10321 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 10381 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 10441 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 10501 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 10561 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 10621 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 10681 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 10741 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 10801 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 10861 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 10921 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 10981 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 11041 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag 11101 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga 11161 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa 11221 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct 11281 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg 11341 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg 11401 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc 11461 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg 11521 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacttcgtg 11581 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa 11641 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 11701 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 11761 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 11821 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 11881 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 11941 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 12001 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 12061 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 12121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 12181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 12241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 12301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 12361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 12421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 12481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 12541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 12601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 12661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 12721 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 12781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 12841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 12901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 12961 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 13021 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 13081 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 13141 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 13201 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 13261 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 13321 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 13381 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 13441 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 13501 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 13561 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 13621 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 13681 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 13741 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 13801 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 13861 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 13921 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 13981 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 14041 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta 14101 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg 14161 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt 14221 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat 14281 atacgcgtt //