LOCUS Exported 6357 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 503 / 569, Emission: 514 / 581. ACCESSION . VERSION . KEYWORDS mGeos2-VESS-Paxillin-22 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6357) TITLE Davidson Photoactivatable Fluorescent Proteins REFERENCE 2 (bases 1 to 6357) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6357 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 52..355 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 356..559 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 510..530 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 2268..2320 /label=MCS /note="multiple cloning site" CDS 2334..3014 /codon_start=1 /product="truly monomeric reversibly photoswitchable fluorescent protein, suitable for nonlinear structured illumination microscopy (Zhang et al., 2016)" /label=Skylan-NS /note="mEos3.1 derivative that eliminates the green-to-red conversion" /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK EGGPLPFAFDILTTAFSYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL EGNAHYRCDSRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD NARR" polyA_signal 3136..3257 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3173..3192) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3227..3246 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3264..3719) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3401..3422) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3613..3632 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3746..3850 /gene="bla" /label=AmpR promoter promoter 3852..4209 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3875..3895) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4060..4195 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4122..4141 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4244..5038 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4298..4317) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4908..4927 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5226..5245) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5270..5317 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5646..6234 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6135..6154 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcacc atggacgacc 601 tcgatgcctt actggcagac ctggaatcca ccacctcgca tatctctaag cgaccggtgt 661 ttctaacaga ggaaacgcct tactcctacc caactggaaa ccatacgtac caggagattg 721 ctgtgccacc gccggtgccc ccgccgcctt ccagtgaggc cctaaatggc actgtgattg 781 accccttaga ccagtggcaa cccagcgtgt ccagatacgg ccaccagcaa cctcagtccc 841 agtctccgat atacagctct agtgccaaaa gctccagtgc ctctgttcct agagacgggc 901 tcagctcccc ttctccccgt gccagtgagg aggaacacgt gtacagcttc ccaaacaagc 961 agaagtctgc agaaccatct cccacaatga ccagcacctc cctgggcagc aacctctcgg 1021 aactggacag gcttctcctg gaactgaatg ctgttcaaca taatcccccc agtggcttct 1081 cagcagatga agtcagcaga agcccatcac tgcccaatgt gaccggacct cactatgtca 1141 tcccagagag cagcagctct gcaggaggga aggctgcacc ccctacaaaa gaaaaaccaa 1201 agcggaatgg tgggcgtggg attgaagatg tgcgtcccag tgtggagagc ctgctggatg 1261 agctggagag ctctgtgcca agcccagtcc ctgcaatcac tgtgagccaa ggggaggtga 1321 gcagccccca gcgtgtcaac gccagtcagc agcagacccg tatctctgct tcttcagcta 1381 cacgagaact ggatgagctg atggcgtccc tctctgactt taagttcatg gcacagggga 1441 aagccggcgg cagcagctcc ccaccatcca ccaccccaaa gcctggcagt cagctggaca 1501 ccatgctggg aagtctgcag tctgacctga acaaactggg ggtagcgacg gtcgccaagg 1561 gtgtgtgcgg agcctgcaag aagccgattg ctgggcaggt agttacagcc atggggaaga 1621 cctggcaccc tgagcacttc gtctgcaccc actgccagga ggagattgga tcacggaact 1681 tctttgagcg ggatgggcag ccctactgtg agaaggacta tcacaacctc ttctctcctc 1741 gctgctacta ctgcaatggg ccaatccttg ataaagtggt gacggctttg gacaggacgt 1801 ggcaccctga acactttttc tgtgcccagt gtggagtttt ctttggacct gaaggatttc 1861 atgagaagga tggcaaagcc tattgccgca aggactactt tgacatgttt gctcccaagt 1921 gtggaggctg tgcccgggct atcctggaaa attacatttc tgccttaaac accctgtggc 1981 accctgagtg ctttgtctgt cgggaatgtt tcacaccgtt catcaacggc agcttcttcg 2041 agcacgacgg gcagccctac tgcgaggtgc attaccacga gcgccgcggc tcgctgtgct 2101 ctggctgcca gaagcccatc acgggacgct gcatcactgc catgggcaag aaatttcacc 2161 ccgaacactt tgtctgtgcc ttctgcctca agcagctcaa caaaggaacc ttcaaagaac 2221 agaacgacaa gccctactgt cagaactgct ttctcaaact cttctgtctc gagctcaagc 2281 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatgagtg 2341 cgattaagcc agacatgaag atcaaactcc gtatggaagg caacgtaaac gggcaccact 2401 ttgtgatcga cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg 2461 aagtcaaaga gggcggacct ctgccttttg cctttgatat cctgaccact gcattctcct 2521 acggcaacag ggtattcgcc aaatatccag acaacataca agactatttt aagcagtcgt 2581 ttcctaaggg gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcaacg 2641 ccagaaacga cataacaatg gaaggggaca ctttctataa taaagttcga ttttatggta 2701 ccaactttcc cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca 2761 ctgagaaaat gtatgtgcgt gatggagtgc tgacgggtga tgtagagatg gctttgttgc 2821 ttgaaggaaa tgcccattac cgatgtgact ccagaactac ttacaaagct aaggagaagg 2881 gtgtcaagtt accaggcgcc cactttgtgg accactgcat tgagatttta agccatgaca 2941 aagattacaa caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca 3001 atgccagacg ataagcggcc gcgactctag atcataatca gccataccac atttgtagag 3061 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 3121 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 3181 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 3241 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 3301 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 3361 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 3421 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 3481 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 3541 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 3601 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 3661 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 3721 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3841 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 3901 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3961 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 4021 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 4081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 4141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 4201 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 4261 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4321 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4381 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 4441 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4501 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4561 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4621 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4681 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4741 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 4801 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4861 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4921 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4981 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 5041 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 5101 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 5161 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 5221 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 5281 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 5341 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 5401 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 5461 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 5521 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5581 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5761 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 5821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6301 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttatta //