LOCUS       Exported                9346 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Induces expression of human MICB allele 005 cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-EF1a-MICB*005-IRES-ZsGreen
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Ferrari de Andrade L, Tay RE, Pan D, Luoma AM, Ito Y, Badrinath S, 
            Tsoucas D, Franz B, May KF Jr, Harvey CJ, Kobold S, Pyrdol JW, Yoon 
            C, Yuan GC, Hodi FS, Dranoff G, Wucherpfennig KW
  TITLE     Antibody-mediated inhibition of MICA and MICB shedding promotes NK 
            cell-driven tumor immunity.
  JOURNAL   Science. 2018 Mar 30;359(6383):1537-1542. doi: 
            10.1126/science.aao0505.
  PUBMED    29599246
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    365..932
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    382..934
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(526..543)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     753..772
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             946..1641
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    1655..2243
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1708..1728)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2126..2137)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2318..2551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2670..2688)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2756..2860
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2861..3721
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3079..3098)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3892..4480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4381..4400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4634..4651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4743..4762
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    4891..4912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4927..4957
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4965..4981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4970..4992
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4989..5005
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4989..5005
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5055..5074
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5266..5899
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5946..6071
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6568..6801
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6986..7030
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7323..7440
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7488..8666
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7719..8657
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8614..8634
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 atgcagggct tccagcttct atccccggaa tatcacactg acctggcgtc aggatggggt
       61 atctttgagc cacaacaccc agcagtgggg ggatgtcctg cctgatggga atggaaccta
      121 ccagacctgg gtggccacca ggattcgcca aggagaggag cagaggttca cctgctacat
      181 ggaacacagc gggaatcatg gcactcaccc tgtgccctct gggaaggcgc tggtgcttca
      241 gagtcaacgg acagactttc catatgtttc tgctgctatg ccatgttttg ttattattat
      301 tattctctgt gtcccttgtt gcaagaagaa aacatcagcg gcagagggtc catagacgcg
      361 tggatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
      421 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
      481 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
      541 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
      601 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
      661 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
      721 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
      781 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
      841 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
      901 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggc ccagtccaag
      961 cacggcctga ccaaggagat gaccatgaag taccgcatgg agggctgcgt ggacggccac
     1021 aagttcgtga tcaccggcga gggcatcggc taccccttca agggcaagca ggccatcaac
     1081 ctgtgcgtgg tggagggcgg ccccttgccc ttcgccgagg acatcttgtc cgccgccttc
     1141 atgtacggca accgcgtgtt caccgagtac ccccaggaca tcgtcgacta cttcaagaac
     1201 tcctgccccg ccggctacac ctgggaccgc tccttcctgt tcgaggacgg cgccgtgtgc
     1261 atctgcaacg ccgacatcac cgtgagcgtg gaggagaact gcatgtacca cgagtccaag
     1321 ttctacggcg tgaacttccc cgccgacggc cccgtgatga agaagatgac cgacaactgg
     1381 gagccctcct gcgagaagat catccccgtg cccaagcagg gcatcttgaa gggcgacgtg
     1441 agcatgtacc tgctgctgaa ggacggtggc cgcttgcgct gccagttcga caccgtgtac
     1501 aaggccaagt ccgtgccccg caagatgccc gactggcact tcatccagca caagctgacc
     1561 cgcgaggacc gcagcgacgc caagaaccag aagtggcacc tgaccgagca cgccatcgcc
     1621 tccggctccg ccttgccctg aatcgataga tcctaatcaa cctctggatt acaaaatttg
     1681 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1741 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1801 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1861 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1921 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1981 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2041 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2101 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2161 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2221 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     2281 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     2341 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     2401 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     2461 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     2521 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     2581 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     2641 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2701 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2761 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2821 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2881 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2941 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3001 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3061 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4681 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
     4741 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4801 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
     4861 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4921 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     4981 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
     5041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     5101 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     5161 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     5221 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     5281 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     5341 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     5401 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     5461 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     5521 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     5581 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     5641 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     5701 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5761 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5821 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5881 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     5941 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     6001 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6061 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6121 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6181 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     6241 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     6301 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     6361 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     6421 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     6481 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     6541 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     6601 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     6661 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     6721 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     6781 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     6841 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     6901 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     6961 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     7021 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     7081 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     7141 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     7201 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     7261 tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac
     7321 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     7381 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     7441 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
     7501 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     7561 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     7621 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     7681 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     7741 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     7801 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     7861 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     7921 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     7981 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     8041 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     8101 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     8161 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     8221 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     8281 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     8341 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     8401 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     8461 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     8521 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     8581 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     8641 tttttcttcc atttcaggtg tcgtgaagcg gccgcatggg gctgggccgg gtcctgctgt
     8701 ttctggccgt cgccttccct tttgcacccc cggcagccgc cgctgagccc cacagtcttc
     8761 gttacaacct catggtgctg tcccaggatg gatctgtgca gtcagggttt ctcgctgagg
     8821 gacatctgga tggtcagccc ttcctgcgct atgacaggca gaaacgcagg gcaaagcccc
     8881 agggacagtg ggcagaagat gtcctgggag ctaagacctg ggacacagag accgaggact
     8941 tgacagagaa tgggcaagac ctcaggagga ccctgactca tatcaaggac cagaaaggag
     9001 gcttgcattc cctccaggag attagggtct gtgagatcca tgaagacagc agcaccaggg
     9061 gctcccggca tttctactac gatggggagc tcttcctctc ccaaaacctg gagactcaag
     9121 aatcgacagt gccccagtcc tccagagctc agaccttggc tatgaacgtc acaaatttct
     9181 ggaaggaaga tgccatgaag accaagacac actatcgcgc tatgcaggca gactgcctgc
     9241 agaaactaca gcgatatctg aaatccgggg tggccatcag gagaacagtg ccccccatgg
     9301 tgaatgtcac ctgcagcgag gtctcagagg gcaacatcac cgtgac
//
