LOCUS       Exported               10876 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase 
            core domain and CASANOVA.
ACCESSION   .
VERSION     .
KEYWORDS    dSpyCas9-p300-P2A-CASANOVA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, 
            Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, 
            Correia BE, Eils R, Niopek D
  TITLE     Engineered anti-CRISPR proteins for optogenetic control of 
            CRISPR-Cas9.
  JOURNAL   Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 
            10.1038/s41592-018-0178-9.
  PUBMED    30377362
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             625..645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             655..4755
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4780..4800
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4810..6660
                     /codon_start=1
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     regulatory      6424..6433
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6661..6687
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6712..6768
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6781..6801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7664..7785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7701..7720)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7755..7774
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7792..8247)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7929..7950)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8141..8160
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8274..8378
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8380..8737
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(8403..8423)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8588..8723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8650..8669
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8772..9566
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8826..8845)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9436..9455
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9754..9773)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9798..9845
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10174..10762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10663..10682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagaggtg
      601 tcgtgacgta cggccaccat gagccccaag aagaagagaa aggtggaggc cagcgacaag
      661 aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac
      721 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      781 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      841 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      901 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      961 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
     1021 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
     1081 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1141 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1201 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     1261 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     1321 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa cctgattgcc
     1381 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     1441 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     1501 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     1561 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     1621 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     1681 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     1741 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     1801 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     1861 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     1921 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     1981 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     2041 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     2101 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     2161 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     2221 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     2281 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     2341 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     2401 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     2461 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     2521 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     2581 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     2641 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     2701 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     2761 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     2821 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     2881 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     2941 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     3001 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     3061 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     3121 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc tatcgtgcct
     3181 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     3241 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     3301 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     3361 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     3421 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     3481 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     3541 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     3601 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     3661 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     3721 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     3781 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     3841 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     3901 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     3961 ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag
     4021 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg
     4081 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     4141 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     4201 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     4261 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga
     4321 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     4381 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     4441 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     4501 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     4561 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc
     4621 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg
     4681 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     4741 cagctgggag gcgacccaat tgccggatcc aaggctagcc cgaaaaagaa acgcaaagtt
     4801 gggcgcgcca ttttcaaacc agaagaacta cgacaggcac tgatgccaac tttggaggca
     4861 ctttaccgtc aggatccaga atcccttccc tttcgtcaac ctgtggaccc tcagctttta
     4921 ggaatccctg attactttga tattgtgaag agccccatgg atctttctac cattaagagg
     4981 aagttagaca ctggacagta tcaggagccc tggcagtatg tcgatgatat ttggcttatg
     5041 ttcaataatg cctggttata taaccggaaa acatcacggg tatacaaata ctgctccaag
     5101 ctctctgagg tctttgaaca agaaattgac ccagtgatgc aaagccttgg atactgttgt
     5161 ggcagaaagt tggagttctc tccacagaca ctgtgttgct acggcaaaca gttgtgcaca
     5221 atacctcgtg atgccactta ttacagttac cagaacaggt atcatttctg tgagaagtgt
     5281 ttcaatgaga tccaagggga gagcgtttct ttgggggatg acccttccca gcctcaaact
     5341 acaataaata aagaacaatt ttccaagaga aaaaatgaca cactggatcc tgaactgttt
     5401 gttgaatgta cagagtgcgg aagaaagatg catcagatct gtgtccttca ccatgagatc
     5461 atctggcctg ctggattcgt ctgtgatggc tgtttaaaga aaagtgcacg aactaggaaa
     5521 gaaaataagt tttctgctaa aaggttgcca tctaccagac ttggcacctt tctagagaat
     5581 cgtgtgaatg actttctgag gcgacagaat caccctgagt caggagaggt cactgttaga
     5641 gtagttcatg cttctgacaa aaccgtggaa gtaaaaccag gcatgaaagc aaggtttgtg
     5701 gacagtggag agatggcaga atcctttcca taccgaacca aagccctctt tgcctttgaa
     5761 gaaattgatg gtgttgacct gtgcttcttt ggcatgcatg ttcaagagta tggctctgac
     5821 tgccctccac ccaaccagag gagagtatac atatcttacc tcgatagtgt tcatttcttc
     5881 cgtcctaaat gcttgaggac tgcagtctat catgaaatcc taattggata tttagaatat
     5941 gtcaagaaat taggttacac aacagggcat atttgggcat gtccaccaag tgagggagat
     6001 gattatatct tccattgcca tcctcctgac cagaagatac ccaagcccaa gcgactgcag
     6061 gaatggtaca aaaaaatgct tgacaaggct gtatcagagc gtattgtcca tgactacaag
     6121 gatattttta aacaagctac tgaagataga ttaacaagtg caaaggaatt gccttatttc
     6181 gagggtgatt tctggcccaa tgttctggaa gaaagcatta aggaactgga acaggaggaa
     6241 gaagagagaa aacgagagga aaacaccagc aatgaaagca cagatgtgac caagggagac
     6301 agcaaaaatg ctaaaaagaa gaataataag aaaaccagca aaaataagag cagcctgagt
     6361 aggggcaaca agaagaaacc cgggatgccc aatgtatcta acgacctctc acagaaacta
     6421 tatgccacca tggagaagca taaagaggtc ttctttgtga tccgcctcat tgctggccct
     6481 gctgccaact ccctgcctcc cattgttgat cctgatcctc tcatcccctg cgatctgatg
     6541 gatggtcggg atgcgtttct cacgctggca agggacaagc acctggagtt ctcttcactc
     6601 cgaagagccc agtggtccac catgtgcatg ctggtggagc tgcacacgca gagccaggac
     6661 tacccgtacg acgttccgga ctacgcttct attaacgcca gcggcagtgg agctacaaac
     6721 tttagccttc tcaaacaagc aggagatgtc gaagagaatc ctggcccggc tagcatggcc
     6781 ccgaagaaaa agcgaaaggt gggcggaagc ggaggttcca tgaacattaa cgacctgatt
     6841 cgggaaataa agaacaagga ctacacggtc aagctgtcag gcaccgactc caactctata
     6901 acacagctca ttataagggt taataatgat ggcaacgaat atgtcatctc tgaatccgag
     6961 aatgaatcaa tcgtggagaa attcatcagt gcatttaaaa acggctggct tgctacaact
     7021 ttggagcgga ttgagaagaa tttcgtcata actgatccca ggttgcctga taatcctata
     7081 atattcgcct ccgatagttt tcttcaactt acggagtact ccagagagga gatactggga
     7141 aggaattgca gattcctcca aggtccagaa actgatagag ctactgtgag gaagatacgg
     7201 gatgccattg acaatcaaac tgaagtgaca gtgcaactta tcaattacac caagtcaggg
     7261 aaaaaattct ggaacctgtt ccatttgcaa cccatgaggg accaaaaggg cgatgtgcaa
     7321 tatttcatag gcgttcaact tgatggtact gaacacgtca gagatgcggc ggagcgagaa
     7381 ggagtgatgc tgattaagaa aacagcagag aatatcgacg aagcggcgaa agagttgtac
     7441 gaagatgagg aagagttcta caatgatatg caaactatca cactgaaatc tgagctgaac
     7501 tagctcgagc ggccgccact gtgctggata tctgcagaat tcgcggccgc gactctagat
     7561 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7621 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     7801 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     7861 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7921 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7981 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8041 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8101 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8161 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8221 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8341 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     8401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8701 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     8761 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     8821 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8881 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8941 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     9001 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9061 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9121 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     9181 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     9241 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     9301 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     9361 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     9421 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     9481 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     9541 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     9601 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9661 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9721 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     9781 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     9841 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     9901 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     9961 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
    10021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    10081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    10141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    10201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    10261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    10321 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    10381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    10441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    10501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    10561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    10621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    10681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    10741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    10801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10861 tattaccgcc atgcat
//
