LOCUS Exported 6639 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Entry vector for adenoviral expression of Ecdysone receptor-based inducible transgene expression switch.. ACCESSION . VERSION . KEYWORDS pENTR-EUI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6639) AUTHORS Lee S, Sohn KC, Choi DK, Won M, Park KA, Ju SK, Kang K, Bae YK, Hur GM, Ro H TITLE Ecdysone Receptor-based Singular Gene Switches for Regulated Transgene Expression in Cells and Adult Rodent Tissues. JOURNAL Mol Ther Nucleic Acids. 2016 Sep 27;5(9):e367. doi: 10.1038/mtna.2016.74. PUBMED 27673563 REFERENCE 2 (bases 1 to 6639) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6639 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" enhancer 837..1216 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1217..1420 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 1371..1391 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 1465..1483 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1503..1940 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain /translation="MKLQSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV" promoter complement(3045..3063) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(3083..3100) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3089..3313 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 4060..4078 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 4103..4119 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 4104..4120 /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter 4459..4477 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" promoter complement(4569..4586) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 4591..4725 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4615..4634) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4669..4688 /label=SV40pA-R /note="SV40 polyA, reverse primer" protein_bind complement(4764..4863) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(4881..4899) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4904..4920) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4904..4920) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(5022..5041) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 5033..5842 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(5100..5119) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 5989..6577 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6478..6497 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agcaggctcc gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 721 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 781 ccgcatagtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 841 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 901 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 961 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 1021 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 1081 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 1141 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 1201 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1261 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 1321 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 1381 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc 1441 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttatcg 1501 atatgaagct acagtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca 1561 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct 1621 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa 1681 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga 1741 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag 1801 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc 1861 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag 1921 gtcaaagaca gttgactgta gagttcctgg atatggccga cttcgagttt gagcagatgt 1981 ttaccgatgc ccttggaatt gacgagtacg gtgggctgca gatgaggcct gaatgtgtca 2041 tacaggagcc cagtaaaaat aaagacaggc aaagacaaaa gaaagacaaa ggaatattat 2101 tacctgttag tacgaccaca gtcgaagacc acatgccccc gatcatgcaa tgtgatccac 2161 ctccgcccga ggccgccagg attcacgaag tcgtcccgag gtatctttcg gagaagctga 2221 tggagcagaa caggcagaag aacataccac cattgtcggc gaatcagaag tctctgatcg 2281 cgaggctcgt gtggtaccag gagggatatg agcagccctc cgacgaggat ctcaaaagag 2341 taacgcagac ttggcagtcg gatgaagagg acgaggaatc cgatctaccc ttccgccaga 2401 tcacggagat gacgatctta acggtccagt tgatcgtcga gttcgccaag ggtctaccgg 2461 gcttttcgaa gatatcacag tctgatcaaa tcaccttatt aaaagcctcg tccagcgagg 2521 tgatgatgct gcgggtggcg aggcgatacg acgccgcgtc cgacagcatt ctgttcgcca 2581 acaacaaggc gtacacgcgc gacaactacc gcaaggcggg catggccgag gtcatcgaag 2641 acctcctaca cttctgccgg tgcatgttcg cgatgggcat ggacaatgtg cactttgcac 2701 tgctcacggc catcgttata ttctcagatc ggcccgggct cgagcagccg tcgctggtag 2761 aagagatcca gagatactac ctgaacacgt tgcgaattta catcatcaac cagaacagcg 2821 cgtcgtcgcg ctgcgccgtg atctacggca ggatcctgag cgtgctgacc gagctacgca 2881 cgctcggcac gcaaaactcc aacatgtgca tctcgctgaa gctgaagaac aggaagctgc 2941 cgccgttcct cgaggagatc tgggacgtgg cggaggtggc cacgacgcat cccacggtgc 3001 tgccgcccac caacccggtg gtgctatagt ctagagggcc ctattctata gtgtcaccta 3061 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 3121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 3181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 3241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 3301 gtgggctcta tggcttctga ggcggaaagt tgagaggaca ttccaatcat aggctgccca 3361 tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat tgggtagggg 3421 tttttcacag accgctttct aagggtaatt ttaaaatatc tgggaagtcc cttccactgc 3481 tgtgttccag aagtgttggt aaacagccca caaatgtcaa cagcagaaac atacaagctg 3541 tcagctttgc acaagggccc aacaccctgc tcatcaagaa gcactgtggt tgctgtgtta 3601 gtaatgtgca aaacaggagg cacattttcc ccacctgtgt aggttccaaa atatctagtg 3661 ttttcatttt tacttggatc aggaacccag cactccactg gataagcatt atccttatcc 3721 aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa ctgtagcatt ttttggggtt 3781 acagtttgag caggatattt ggtcctgtag tttgctaaca caccctgcag ctccaaaggt 3841 tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa tgggttttcc agcaccattt 3901 tcatgagttt tttgtgtccc tgaatgcaag tttaacatag cagttacccc aataacctca 3961 gttttaacag taacagcttc ccacatcaaa atatttccac aggttaagtc ctcatttaaa 4021 ttaggcaaag gaattgctca actttgtaag aaaagttggt aatacgactc actatagggc 4081 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttag gcctccaagg 4141 cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg agtactgtcc 4201 tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg tcctccgggc 4261 tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag aggtcggagt 4321 actgtcctcc gacgcaaggc ggagtactgt cctccgggct ggcggagtac tgtcctccgg 4381 caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct cactttgagc 4441 tcctccacac ggagcttgat ttaggtgaca ctatagaata caagctactt gttctttttg 4501 caggatccca tcgattcgaa ttcacgtgct agcgcttaag aaattcaagg cctctcgagc 4561 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag 4621 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 4681 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 4741 attcatttta tgtttcaggg ccgacccagc tttcttgtac aaagttggca ttataagaaa 4801 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat 4861 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 4921 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 4981 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 5041 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 5101 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 5161 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 5221 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 5281 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 5341 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 5401 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 5461 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 5521 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 5581 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 5641 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 5701 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 5761 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 5821 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 5881 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 5941 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 6001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 6121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6601 acggttcctg gccttttgct ggccttttgc tcacatgtt //