LOCUS       Exported                5952 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Localization: Membrane, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    mIFP12-Annexin-12
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        52..355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        356..559
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     510..530
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             604..1563
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    2731..2852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2768..2787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2822..2841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2859..3314)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2996..3017)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3208..3227
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3341..3445
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3447..3804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3470..3490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3655..3790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3717..3736
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3839..4633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3893..3912)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4503..4522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4821..4840)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4865..4912
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5241..5829
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5730..5749
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc
      601 accatgtcgg taccgctgac tacctcagca ttcggccacg cgtttctggc taactgtgaa
      661 cgcgagcaga tccacctggc gggctccatt cagccgcacg gtatcctgct ggctgtgaaa
      721 gagccggaca acgtggtgat ccaggcttct attaacgctg cggagttcct gaacaccaac
      781 tctgttgttg gccgtccgct gcgtgacctg ggcggcgatc tgcctttgca gatcctgccg
      841 cacctgaacg gcccgctgca cctggctccg atgaccctgc gttgtaccgt gggttctccg
      901 ccgcgtcgtg tggactgtac cattcatcgt ccgtctaacg gcggcctgat cgtagaactg
      961 gaaccagcaa ccaagaccac taacattgcg ccggctctgg acggtgcgtt tcatcgtatc
     1021 acttcttcat cctccctgat gggcctgtgt gacgaaaccg cgactattat ccgtgagatt
     1081 actggctacg accgtgtgat ggtagtacgt ttcgatgaag agggtaatgg cgaaattctg
     1141 tccgaacgtc gtcgtgcgga cctggaagcg ttcctgggta accgctaccc ggcgtctact
     1201 attccgcaga tcgctcgtcg cctgtacgaa cataaccgtg ttcgcctgct ggtagatgtg
     1261 aactatactc cggttccgct acagccgcgc atcagcccgc tgaacggtcg tgatctggat
     1321 atgtccctgt cttgcctgcg ctctatgtcc ccgatccacc agaaatacat gcaggacatg
     1381 ggcgttggcg cgaccctggt ttgctctctg atggtgtctg gtcgtctgtg gggtctgatc
     1441 gcttgccacc actacgaacc gcgcttcgtt ccgttccaca ttcgcgctgc tggcgaagcg
     1501 ctggcggaaa cttgtgcgat ccgcatcgcg acgctggaga gctttgcaca gtctcagtcc
     1561 aaaggtggcg gaagcggcgg cggaagcggt ggcggaagtt ccggactcag atctcgagct
     1621 caagcttcga attctatggc catggcaacc aaaggaggta ctgtcaaagc tgcttcagga
     1681 ttcaatgcca tggaagatgc ccagaccctg aggaaggcca tgaaagggct cggcaccgat
     1741 gaagacgcca ttattagcgt ccttgcctac cgcaacaccg cccagcgcca ggagatcagg
     1801 acagcctaca agagcaccat cggcagggac ttgatagacg acctgaagtc agaactgagt
     1861 ggcaacttcg agcaggtgat tgtggggatg atgacgccca cggtgctgta tgacgtgcaa
     1921 gagctgcgaa gggccatgaa gggagccggc actgatgagg gctgcctaat tgagatcctg
     1981 gcctcccgga cccctgagga gatccggcgc ataagccaaa cctaccagca gcaatatgga
     2041 cggagccttg aagatgacat tcgctctgac acatcgttca tgttccagcg agtgctggtg
     2101 tctctgtcag ctggtgggag ggatgaagga aattatctgg acgatgctct cgtgagacag
     2161 gatgcccagg acctgtatga ggctggagag aagaaatggg ggacagatga ggtgaaattt
     2221 ctaactgttc tctgttcccg gaaccgaaat cacctgttgc atgtgtttga tgaatacaaa
     2281 aggatatcac agaaggatat tgaacagagt attaaatctg aaacatctgg tagctttgaa
     2341 gatgctctgc tggctatagt aaagtgcatg aggaacaaat ctgcatattt tgctgaaaag
     2401 ctctataaat cgatgaaggg cttgggcacc gatgataaca ccctcatcag agtgatggtt
     2461 tctcgagcag aaattgacat gttggatatc cgggcacact tcaagagact ctatggaaag
     2521 tctctgtact cgttcatcaa gggtgacaca tctggagact acaggaaagt actgcttgtt
     2581 ctctgtggag gagatgatta aggatccacc ggatctagat aactgatcat aatcagccat
     2641 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2701 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2761 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2821 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     2881 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     2941 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3001 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3061 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3121 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3181 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3241 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3301 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3361 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3421 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3481 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3541 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3601 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3661 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3721 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3781 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3841 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3901 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3961 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4021 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4081 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4141 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4201 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4261 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4321 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4381 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4441 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4501 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4561 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4621 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4681 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4741 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4801 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4861 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     4921 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     4981 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5041 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5101 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5161 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5221 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5281 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5341 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5401 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5461 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5521 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5581 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5641 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5701 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5761 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5821 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5881 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     5941 cattagttat ta
//
