LOCUS Exported 11440 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION TET-inducible expression of a dominant negative form of the TCF4 transcription factor. ACCESSION . VERSION . KEYWORDS pCWXPGR-pTF-dnTCF4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11440) AUTHORS Bocchi R, Egervari K, Carol-Perdiguer L, Viale B, Quairiaux C, De Roo M, Boitard M, Oskouie S, Salmon P, Kiss JZ TITLE Perturbed Wnt signaling leads to neuronal migration delay, altered interhemispheric connections and impaired social behavior. JOURNAL Nat Commun. 2017 Oct 27;8(1):1158. doi: 10.1038/s41467-017-01046-w. PUBMED 29079819 REFERENCE 2 (bases 1 to 11440) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11440 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..24 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" protein_bind complement(1741..1765) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter 1782..2292 /label=hPGK promoter /note="human phosphoglycerate kinase 1 promoter" primer_bind 2211..2229 /label=hPGK-F /note="Human PGK promoter, forward primer" regulatory 2308..2317 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2314..3030 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2314..3030 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2359..2380) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2620..2639) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2967..2988 /label=EGFP-C /note="EGFP, forward primer" CDS 3046..3105 /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS 4174..4545 /codon_start=1 /product="transcriptional activation domain of human RelA, also known as p65 (O'Shea and Perkins, 2008)" /label=RelA (p65) AD /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD EDFSSIADMDFSALLSQISS" misc_feature 4571..5159 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4624..4644) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5042..5053) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5162..5178) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5163..5179) /label=pBluescriptKS /note="For pBluescript vector" protein_bind 5307..5340 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 5360..5540 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5578..5597) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5643..5698 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5697..5716) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(6059..6075) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6059..6075) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6072..6094) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6083..6099 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6107..6137) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6152..6173 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6232..6427 /label=SV40 promoter /note="SV40 early promoter" rep_origin 6278..6413 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6340..6359 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 6433..6567 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6470..6489) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6524..6543 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6635..6652) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6806..7393) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6886..6905) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7564..8424) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8187..8206 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8425..8529) /gene="bla" /label=AmpR promoter enhancer 8560..8939 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" LTR 8941..9198 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 9245..9370 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9862..9979 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" misc_feature 10339..10572 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 10757..10801 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" protein_bind 11076..11096 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 11123..11141 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" protein_bind 11164..11182 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" protein_bind 11328..11346 /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O1 for the tetR and tetA genes" protein_bind 11408..11432 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 gactacaaag acgatgacga taaaaaggat ccgagctcga attccgaaaa ctcctcggca 61 gagagggatt tagctgatgt caaatcgtct ctagtcaatg aatcagaaac gaatcaaaac 121 agctcctccg attccgaggc ggaaagacgg cctccgcctc gctccgaaag tttccgagac 181 aaatcccggg aaagtttgga agaagcggcc aagaggcaag atggagggct ctttaagggg 241 ccaccgtatc ccggctaccc cttcatcatg atccccgacc tgacgagccc ctacctcccc 301 aacggatcgc tctcgcccac cgcccgaacc tatctccaga tgaaatggcc actgcttgat 361 gtccaggcag ggagcctcca gagtagacaa gccctcaagg atgcccggtc cccatcaccg 421 gcacacattg tctctaacaa agtgccagtg gtgcagcacc ctcaccatgt ccaccccctc 481 acgcctctta tcacgtacag caatgaacac ttcacgccgg gaaacccacc tccacactta 541 ccagccgacg tagaccccaa aacaggaatc ccacggcctc cgcaccctcc agatatatcc 601 ccgtattacc cactatcgcc tggcaccgta ggacaaatcc cccatccgct aggatggtta 661 gtaccacagc aaggtcaacc agtgtaccca atcacgacag gaggattcag acacccctac 721 cccacagctc tgaccgtcaa tgcttccatg tccaggttcc ctccccatat ggtcccacca 781 catcatacgc tacacacgac gggcattccg catccggcca tagtcacacc aacagtcaaa 841 caggaatcgt cccagagtga tgtcggctca ctccatagtt caaagcatca ggactccaaa 901 aaggaagaag aaaagaagaa gccccacata aagaaacctc ttaatgcatt catgttgtat 961 atgaaggaaa tgagagcaaa ggtcgtagct gagtgcacgt tgaaagaaag cgcggccatc 1021 aaccagatcc ttgggcggag gtggcatgca ctgtccagag aagagcaagc gaaatactac 1081 gagctggccc ggaaggagcg acagcttcat atgcaactgt accccggctg gtccgcgcgg 1141 gataactatg gaaagaagaa gaagaggaaa agggacaagc agccgggaga gaccaatgaa 1201 cacagcgaat gtttcctaaa tccttgcctt tcacttcctc cgattacaga cctgagcgct 1261 cctaagaaat gccgagcgcg ctttggcctt gatcaacaga ataactggtg cggcccttgc 1321 aggagaaaaa aaaagtgcgt tcgctacata caaggtgaag gcagctgcct cagcccaccc 1381 tcttcagatg gaagcttact agattcgcct cccccctccc cgaacctgct aggctcccct 1441 ccccgagacg ccaagtcaca gactgagcag acccagcctc tgtcgctgtc cctgaagccc 1501 gaccccctgg cccacctgtc catgatgcct ccgccacccg ccctcctgct cgctgaggcc 1561 acccacaagg cctccgccct ctgtcccaac ggggccctgg acctgccccc agccgctttg 1621 cagcctgccg ccccctcctc atcaattgca cagccgtcga cttcttcctt acattcccac 1681 agctccctgg ccgggaccca gccccagccg ctgtcgctcg tcaccaagtc tttagaatag 1741 acccagcttt cttgtacaaa gtggtgataa tcgaattcca cggggttggg gttgcgcctt 1801 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa 1861 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg 1921 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg 1981 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact 2041 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct 2101 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg 2161 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca 2221 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac 2281 ctctctcccc agggggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc 2341 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 2401 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 2461 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 2521 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 2581 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 2641 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 2701 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 2761 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 2821 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 2881 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 2941 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 3001 atcactctcg gcatggacga gctgtacaag gctagcggat caggtcaatg tactaactac 3061 gctttgttga agcttgccgg cgatgttgaa agtaaccccg gtcctggatc catgtccaga 3121 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa 3181 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg 3241 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat 3301 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc 3361 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc 3421 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa 3481 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc 3541 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc 3601 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa 3661 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc 3721 gaaagcggcg ggccggcctc gagcgaattc cagtacctgc cagatacaga cgatcgtcac 3781 cggattgagg agaaacgtaa aaggacatat gagaccttca agagcatcat gaagaagagt 3841 cctttcagcg gacccaccga cccccggcct ccacctcgac gcattgctgt gccttcccgc 3901 agctcagctt ctgtccccaa gccagcaccc cagccctatc cctttacgtc atccctgagc 3961 accatcaact atgatgagtt tcccaccatg gtgtttcctt ctgggcagat cagccaggcc 4021 tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc tgcccctgct 4081 ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct agccccaggc 4141 cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga aggaacgctg 4201 tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt gcttggcaac 4261 agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga gtttcagcag 4321 ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct gatggagtac 4381 cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc agctcctgct 4441 ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga cttctcctcc 4501 attgcggaca tggacttctc agccctgctg agtcagatca gctcctaaag atctgatcaa 4561 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa 4621 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat 4681 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta 4741 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc 4801 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt 4861 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg 4921 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc 4981 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc 5041 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct 5101 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca 5161 tcgataccgt cgacctcgag gttcgcttta agaccaatga cttacaaggc agctgtagat 5221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga 5281 caagatggga tcaattcacc atgggaataa cttcgtatag catacattat acgaagttat 5341 gctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 5401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 5461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 5521 agtgtggaaa atctctagca gcatctagtt gaatcgccag tgtgtcgagc tagcagatct 5581 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 5641 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 5701 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 5761 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 5821 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 5881 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 5941 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 6001 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 6061 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6121 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6181 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cgcatctcaa 6241 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 6301 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 6361 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 6421 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 6481 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 6541 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga 6601 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6661 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6721 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6781 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6901 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt acggatacct 6961 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 7021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 7081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7141 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7201 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 7261 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7321 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7381 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7441 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7501 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7561 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7621 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7681 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7741 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7801 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7861 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7921 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 7981 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8041 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8101 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8161 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8221 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8281 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8341 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8401 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8461 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8521 ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt 8581 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta 8641 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 8701 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 8761 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 8821 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 8881 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 8941 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc 9001 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 9061 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 9121 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 9181 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga 9241 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg 9301 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg 9361 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag 9421 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga 9481 acgattcgca gttaatactg gcctgttaga aacatcagaa ggctgtagac aaatactggg 9541 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt 9601 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga 9661 caagatagag gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag caggatcttc 9721 agacctggaa attccctaca atccccaaag tcaaggagta gtagaatcta tgaataaaga 9781 attaaagaaa attataggac aggtaagaga tcaggctgaa catcttaaga cagcagtaca 9841 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg 9901 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat 9961 tacaaaaatt caaaattttc gggtttatta cagggacagc agaaatccac tttggaaagg 10021 accagcaaag ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat 10081 aaaagtagtg ccaagaagaa aagcaaagat cattagggat tatggaaaac agatggcagg 10141 tgatgattgt gtggcaagta gacaggatga ggattagaac atggaaaagt ttagtaaaac 10201 accataagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt 10261 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga 10321 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac 10381 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt 10441 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac 10501 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga 10561 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc 10621 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat 10681 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc 10741 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt 10801 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt 10861 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag 10921 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag 10981 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt 11041 gaacggatct cgacggtatc tagtgattat cggatcaact ttgtatagaa aagttgggta 11101 caactcccac tagacatatc ctactctatc attgatagag tgacccttcc atagacatat 11161 cctactctat cattgataga gtgacccttc cactaggttc acttttctct atcattgata 11221 gggagtggta aacaattgat ggacaaaatc tatcaatgat agagagtgca tgttactagt 11281 attgttcttc tctatcattg atagtgactt tgcctaatca cttagcaact ctatcattga 11341 tagagttcta gtattagcaa ggcctttcct ctatataagg aagttcattt catttggaga 11401 agatccccca agtttgtaca aaaaagcagg cttcaccatg //