LOCUS Exported 11373 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.. ACCESSION . VERSION . KEYWORDS Ccas9LacZ SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11373) AUTHORS Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T TITLE Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. JOURNAL Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. PUBMED 29924347 REFERENCE 2 (bases 1 to 11373) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11373 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(6..23) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(6..22) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 43..392 /label=CeU6 promoter /note="promoter for the C. elegans R07E5.16 U6 snRNA gene (Dickinson et al., 2013)" CDS 505..3552 /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /label=lacZ /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" misc_RNA 3654..3729 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 4001..4023 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4061..4079) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4147..4251 /gene="bla" /label=AmpR promoter CDS 4252..5112 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4470..4489) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5283..5871 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5772..5791 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5998..6020 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6017..6033 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6017..6033 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" protein_bind 6053..6073 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" promoter 6074..6673 /gene="eft-3" /label=eft-3 promoter /note="promoter for the C. elegans elongation factor 1-alpha gene (Mitrovich and Anderson, 2000)" CDS join(6690..7733,7785..8855,8907..9932,9984..10946) /codon_start=1 /product="S. pyogenes Cas9, codon optimized for C. elegans with synthetic introns" /label=Cas9 /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 10962..10982 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 10983..11009 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" terminator 11024..11347 /gene="tbb-2" /label=tbb-2 terminator /note="transcription terminator for the C. elegans tubulin beta-2 gene" protein_bind complement(11351..11371) /gene="mutant version of attB" /label=attB3 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" ORIGIN 1 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accaaaaaaa actagcaata 61 aaggaataaa aaactgtaca ccttaaaggc gcacactctg ttttgcaaat tttattttta 121 gttgtgaatt ttctgctgag acctgaaaat agcaacttta gtactactat aatttgtcaa 181 ccttttcaaa aaaagcatgc aatttttgag aaactcttat aaaagctatt attaaaaaaa 241 cacctttttt ccaaaattat tccacaaaaa atatgttatg aaatgcctac accctctcac 301 acacactctt tatactactc tgtcaaactc acgagatgtc tgccgcctct tgtgttgccc 361 ctatataaac acctcctatt gcgagatgtc ttggcaggtg gaggtcgacg gtatcgataa 421 gcttagtctc cgaaggaacg tgtcacggtt gacagcagtc ctaggtataa tactagtgga 481 ctttaagaag gagatataca tatggtcgtt ttacaacgtc gtgactggga aaaccctggc 541 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 601 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt 661 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 721 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac 781 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg 841 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga 901 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt 961 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 1021 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag 1081 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca 1141 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg 1201 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta 1261 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 1321 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg 1381 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc 1441 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt 1501 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat 1561 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 1621 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg 1681 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg 1741 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta 1801 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat 1861 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 1921 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc 1981 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc 2041 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac 2101 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc 2161 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 2221 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag 2281 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca 2341 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc 2401 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt 2461 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 2521 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta 2581 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg 2641 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg 2701 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc 2761 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 2821 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc 2881 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca 2941 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac 3001 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt 3061 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 3121 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta 3181 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg 3241 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg 3301 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc 3361 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 3421 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc 3481 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg 3541 tgtcaaaaat aataaccctt atcaacttga aaaagtggca ccgagtcggt gcttttttcc 3601 gtgacacgtt ccttcggaga ctctagagcg gccgccaccg aagcttcacc tgcgttttag 3661 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 3721 agtcggtgct ttttgtgaaa tttctggcgt aatagcgaag aggcccgcac cgatcgccct 3781 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 3841 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtgcaatctg ctctgatgcc 3901 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 3961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 4021 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 4081 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 4141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 4201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 4261 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4321 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 4381 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 4441 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 4501 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 4561 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 4621 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 4681 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4741 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4801 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4861 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4921 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4981 attgcagcac tggggtcaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 5041 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 5101 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 5161 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5341 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5401 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 5461 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5521 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 5581 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 5641 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 5701 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5761 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5821 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 5881 aacgcggcct ttttacggtt cctggtcttt tgctggcctt ttgctcacat gttctttcct 5941 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccagc 6001 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atcaactttg 6061 tatagaaaag ttggcacctt tggtctttta ttgtcaactt ccattggttc ttccattgtt 6121 tctgttaaat taatgaattt ttcataaaat aaagacatta tacaatataa aaatgaagaa 6181 tttattgaaa ataaactgcc agagagaaaa agtatgcaac actcccgccg agagtgtttg 6241 aaatggtgta cggtacattt tcgtgctagg agttagatgt gcaggcagca acgagagggg 6301 gagagatttt tttgggcctt gtgaaattaa cgtgagtttt ctggtcatct gactaatcat 6361 gttggttttt tgttggttta ttttgttttt atctttgttt ttatccagat taggaaattt 6421 aaattttatg aatttataat gaggtcaaac attcagtccc agcgtttttc ctgttctcac 6481 tgtttagtcg aatttttatt ttaggctttc aacaaatgtt ctaactgtct tatttgtgac 6541 ctcacttttt atattttttt aatttttaaa aatattagaa gtttctagga taattttttc 6601 gacttttatt ctctctaccg tccgcactct tcttactttt aaattaaatt gttttttttt 6661 cagttgggaa acactttgct ctagaaaaaa tggacaaaaa atacagcatc ggccttgaca 6721 tcggaacgaa ctccgtcgga tgggccgtca tcaccgacga gtacaaggtc ccatccaaga 6781 agttcaaggt cctcggaaac accgaccgtc actccatcaa gaagaacctc atcggagccc 6841 tcctcttcga ctccggagag accgccgagg ccacccgtct caagcgtacc gcccgtcgtc 6901 gttacacccg tcgtaagaac cgtatctgct acctccaaga gatcttctcc aacgagatgg 6961 ccaaggtcga cgactccttc ttccaccgtc tcgaggagtc cttcctcgtc gaggaggaca 7021 agaagcacga gcgtcaccca atcttcggaa acatcgtcga cgaggtcgcc taccacgaga 7081 agtacccaac catctaccac ctccgtaaga agctcgtcga ctccaccgac aaggccgacc 7141 tccgtctcat ctacctcgcc ctcgcccaca tgatcaagtt ccgtggacac ttcctcatcg 7201 agggagacct caacccagac aactccgacg tcgacaagct cttcatccaa ctcgtccaaa 7261 cctacaacca actcttcgag gagaacccaa tcaacgcctc cggagtcgac gccaaggcca 7321 tcctctccgc ccgtctctcc aagtcccgtc gtctcgagaa cctcatcgcc caactcccag 7381 gagagaagaa gaacggactc ttcggaaacc tcatcgccct ctccctcgga ctcaccccaa 7441 acttcaagtc caacttcgac ctcgccgagg acgccaagct ccaactctcc aaggacacct 7501 acgacgacga cctcgacaac ctcctcgccc aaatcggaga ccaatacgcc gacctcttcc 7561 tcgccgccaa gaacctctcc gacgccatcc tcctctccga catcctccgt gtcaacaccg 7621 agatcaccaa ggccccactc tccgcctcca tgatcaagcg ttacgacgag caccaccaag 7681 acctcaccct cctcaaggcc ctcgtccgtc aacaactccc agagaagtac aaggtaagtt 7741 taaacatata tatactaact aaccctgatt atttaaattt tcaggagatc ttcttcgacc 7801 aatccaagaa cggatacgcc ggatacatcg acggaggagc ctcccaagag gagttctaca 7861 agttcatcaa gccaatcctc gagaagatgg acggaaccga ggagctcctc gtcaagctca 7921 accgtgagga cctcctccgt aagcaacgta ccttcgacaa cggatccatc ccacaccaaa 7981 tccacctcgg agagctccac gccatcctcc gtcgtcaaga ggacttctac ccattcctca 8041 aggacaaccg tgagaagatc gagaagatcc tcaccttccg tatcccatac tacgtcggac 8101 cactcgcccg tggaaactcc cgtttcgcct ggatgacccg taagtccgag gagaccatca 8161 ccccatggaa cttcgaggag gtcgtcgaca agggagcctc cgcccaatcc ttcatcgagc 8221 gtatgaccaa cttcgacaag aacctcccaa acgagaaggt cctcccaaag cactccctcc 8281 tctacgagta cttcaccgtc tacaacgagc tcaccaaggt caagtacgtc accgagggaa 8341 tgcgtaagcc agccttcctc tccggagagc aaaagaaggc catcgtcgac ctcctcttca 8401 agaccaaccg taaggtcacc gtcaagcaac tcaaggagga ctacttcaag aagatcgagt 8461 gcttcgactc cgtcgagatc tccggagtcg aggaccgttt caacgcctcc ctcggaacct 8521 accacgacct cctcaagatc atcaaggaca aggacttcct cgacaacgag gagaacgagg 8581 acatcctcga ggacatcgtc ctcaccctca ccctcttcga ggaccgtgag atgatcgagg 8641 agcgtctcaa gacctacgcc cacctcttcg acgacaaggt catgaagcag cttaagcgtc 8701 gtcgttacac cggatgggga cgtctctccc gtaagctcat caacggaatc cgtgacaagc 8761 aatccggaaa gaccatcctc gacttcctca agtccgacgg attcgccaac cgtaacttca 8821 tgcaactcat ccacgacgac tccctcacct tcaaggtaag tttaaacagt tcggtactaa 8881 ctaaccatac atatttaaat tttcaggagg acatccaaaa ggcccaagtc tccggacaag 8941 gagactccct ccacgagcac atcgccaacc tcgccggatc cccagccatc aagaagggaa 9001 tcctccaaac cgtcaaggtc gtcgacgagc tcgtcaaggt catgggacgt cacaagccag 9061 agaacatcgt catcgagatg gcccgtgaga accaaaccac ccaaaaggga caaaagaact 9121 cccgtgagcg tatgaagcgt atcgaggagg gaatcaagga gctcggatcc caaatcctca 9181 aggagcaccc agtcgagaac acccaactcc aaaacgagaa gctctacctc tactacctcc 9241 aaaacggacg tgacatgtac gtcgaccaag agctcgacat caaccgtctc tccgactacg 9301 acgtcgacca catcgtccca caatccttcc tcaaggacga ctccatcgac aacaaggtcc 9361 tcacccgttc cgacaagaac cgtggaaagt ccgacaacgt cccatccgag gaggtcgtca 9421 agaagatgaa gaactactgg cgtcaactcc tcaacgccaa gctcatcacc caacgtaagt 9481 tcgacaacct caccaaggct gagcgtggag gactctccga gctcgacaag gccggattca 9541 tcaagcgtca actcgtcgag acccgtcaaa tcaccaagca cgtcgcccaa atcctcgact 9601 cccgtatgaa caccaagtac gacgagaacg acaagctcat ccgtgaggtc aaggtcatca 9661 ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccaattctac aaggtccgtg 9721 agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtagta ggaaccgccc 9781 tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac aaggtctacg 9841 acgtccgtaa gatgatcgcc aagtccgagc aagagatcgg aaaggccacc gccaagtact 9901 tcttctactc caacatcatg aacttcttca aggtaagttt aaacatgatt ttactaacta 9961 actaatctga tttaaatttt cagaccgaga tcaccctcgc caacggagag atccgtaagc 10021 gtccactcat cgagaccaac ggagagaccg gagagatcgt ctgggacaag ggacgtgact 10081 tcgccacagt ccgtaaggtc ctctccatgc cacaagtcaa catcgtcaag aagaccgagg 10141 tccaaaccgg aggattctcc aaggagtcca tcctcccaaa gcgtaactcc gacaagctca 10201 tcgcccgtaa gaaggactgg gacccaaaga agtacggagg attcgactcc ccaaccgtcg 10261 cctactccgt cctcgtcgtc gccaaggtcg agaagggaaa gtccaagaag ctcaagtccg 10321 tcaaggagct cctcggaatc accatcatgg agcgttcctc cttcgagaag aacccaatcg 10381 acttcctcga ggccaaggga tacaaggagg tcaagaagga cctcatcatc aagctcccaa 10441 agtactccct cttcgagctc gagaacggac gtaagcgtat gctcgcctcc gccggagagc 10501 tccaaaaggg aaacgagctc gccctcccat ccaagtacgt caacttcctc tacctcgcct 10561 cccactacga gaagctcaag ggatccccag aggacaacga gcaaaagcaa ctcttcgtcg 10621 agcaacacaa gcactacctc gacgagatca tcgagcaaat ctccgagttc tccaagcgtg 10681 tcatcctcgc cgacgccaac ctcgacaagg tcctctccgc ctacaacaag caccgtgaca 10741 agccaatccg tgagcaagcc gagaacatca tccacctctt caccctcacc aacctcggag 10801 ccccagccgc cttcaagtac ttcgacacca ccatcgaccg taagcgttac acctccacca 10861 aggaggtcct cgacgccacc ctcatccacc aatccatcac cggactctac gagacccgta 10921 tcgacctctc ccaactcgga ggagacggag gatccggacc accaaagaag aagcgtaagg 10981 tctacccata cgacgtccca gactacgcct aaactagtga taaatgcaaa atcctttcaa 11041 gcattccctt cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt 11101 atttgctttt ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct 11161 gagtgaagtg aatgctatca aaatgtgatt ctgtctgatg tactttcaca atctctcttc 11221 aattccattt tgaagtgctt taaacccgaa aggttgagaa aaatgcgagc gctcaaatat 11281 ttgtattgtg ttcgttgagt gacccaacaa aaagaggaaa ctttattgtg ccgccaagaa 11341 aaaagtctca caactttatt atacatagtt gat //