LOCUS       Exported                4027 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  This plasmid is part of the Minimal Antibiotic Resistance Platform 
            (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that 
            constitutively expresses genes using the Pbla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    pGDP3 apmA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, 
            Wright GD
  TITLE     A Common Platform for Antibiotic Dereplication and Adjuvant 
            Discovery.
  JOURNAL   Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 
            10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.
  PUBMED    28017602
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             273..290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             300..317
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1152..1169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1231..1258
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1283..1387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1388..2248
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1606..1625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2419..3007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2908..2927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3161..3178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3193..3332
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3258..3277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3418..3440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3434..3625)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
       61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
      121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
      241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
      301 tggtgccgcg cggcagccat atgaaaacca gacttgaaca agttttagaa cgttatctca
      361 acggacgcga ggtagctgta tggggtgttc cgacacgccg tttgcttcgt gcattaaaac
      421 cttttaaatt tcatactgcc gatcgtgttg atcctcaata tcattatgtt gttgctgtaa
      481 ccgatgacga cttaaccgac tttttatccg atgaacagag caagtcgttt caatacgcga
      541 atgactacct tacgtttgac gacgagggag gcgaacttcc gtttgaacga atgtgcttca
      601 atgtccccgt tggcaggcaa acgtattttg gagacggtgt cgtaggagct tgcgaaaacg
      661 ggtatatcaa gagcatcggc caatttacat cgattaacgg cacggcagag atccatgcga
      721 accatcagtt aaacatgacc tttgtaagcg acgatattca aaacttcttc aacgaagaaa
      781 gcatggctgt attccaagaa aagctgcgaa aagacccgaa acacccttat gcttatagca
      841 aagaacctat gaccataggt agcgatgttt atatcggcgc acatgcattc atcaatgctt
      901 ccactgtgac aagcatcggt gacggtgcaa taattggctc aggggcagta gttctggaaa
      961 atgttcctcc ttttgctgtc gtcgttggtg tccctgcgag aatcaagcgt taccgcttct
     1021 caaaggagat gattgaaacc ctccttcgtg tcaaatggtg ggactggagt atagaggaaa
     1081 ttaacgaaaa tgttgatgcg cttatatcac ctgaactatt tatgaaaaag tacgggagtt
     1141 tgtaactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
     1201 aagctgagtt ggctgctgcc accgctgagc aaaaggccat ccgtcaggat ggccttctgg
     1261 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1321 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1381 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1441 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1501 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1561 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1621 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1681 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1741 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1801 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1861 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1921 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1981 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2041 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2101 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2161 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2221 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3121 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3181 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3241 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     3301 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     3361 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     3421 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     3481 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     3541 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     3601 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     3661 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     3721 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     3781 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     3841 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     3901 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     3961 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     4021 gctaacc
//
