LOCUS Exported 3670 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.. ACCESSION . VERSION . KEYWORDS pGDP3 arr SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3670) AUTHORS Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, Wright GD TITLE A Common Platform for Antibiotic Dereplication and Adjuvant Discovery. JOURNAL Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22. PUBMED 28017602 REFERENCE 2 (bases 1 to 3670) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3670 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 232..254 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 273..290 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 300..317 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 795..812 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" terminator 874..901 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 926..1030 /gene="bla" /label=AmpR promoter CDS 1031..1891 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1249..1268) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2062..2650 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2551..2570 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2804..2821 /label=L4440 /note="L4440 vector, forward primer" misc_feature 2836..2975 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(2901..2920) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3061..3083 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(3077..3268) /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" ORIGIN 1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg 61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca 121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa 241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc 301 tggtgccgcg cggcagccat atggtaaaag attggattcc catctctcat gataattaca 361 agcaggtgca aggaccgttc tatcatggaa ccaaagccaa tttggcgatt ggtgacttgc 421 taaccacagg gttcatctct catttcgagg acggtcgtat tcttaagcac atctactttt 481 cagccttgat ggagccagca gtttggggag ctgaacttgc tatgtcactg tctggcctcg 541 agggtcgcgg ctacatatac atagttgagc caacaggacc gttcgaagac gatccgaatc 601 ttacgaacaa aaaatttccc ggtaatccaa cacagtccta tagaacctgc gaacccttga 661 gaattgttgg cgttgttgaa gactgggagg ggcatcctgt tgaattaata aggggaatgt 721 tggattcgtt agaggactta aagcgccgtg gtttacacgt cattgaagac tagaagcttg 781 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa 841 aggaagctga gttggctgct gccaccgctg agcaaaaggc catccgtcag gatggccttc 901 tggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 961 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 1021 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt 1081 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca 1141 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag 1201 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc 1261 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca 1321 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt 1381 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct 1441 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt 1501 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga 1561 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact 1621 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc 1681 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga 1741 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt 1801 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga 1861 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact 1921 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga 1981 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 2041 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 2101 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 2161 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 2221 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 2281 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 2341 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 2401 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 2461 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 2521 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 2581 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 2641 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 2701 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 2761 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 2821 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 2881 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca 2941 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg 3001 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 3061 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc 3121 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt 3181 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt 3241 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca 3301 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg 3361 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg 3421 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc 3481 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg 3541 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc 3601 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat 3661 tctgctaacc //