LOCUS       Exported               10527 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Muscle specific zebrafish expression of mKate2 fused to EHD1b.
ACCESSION   .
VERSION     .
KEYWORDS    actc1b-mKate2-EHD1b
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  TITLE     Parton Lab Plasmids for in vivo cell biology in the zebrafish
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..25
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      26..35
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(729..753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2351..2371)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2379..2395)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2411..2545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2435..2454)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2489..2508
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3217..3233)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3217..3233)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3270..3290)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3270..3288)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3309..3325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3322..3344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3333..3349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3357..3387)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3402..3423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3540..3557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3711..4299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3791..3810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4470..5330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5093..5112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5331..5435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5598..5619)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6515..6531
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6551..6571
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gcaagtttgt acaaaaaagc aggctgccac catggtgagc gagctgatta aggagaacat
       61 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggcgg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcaaaacctt
      241 catcaaccac acccagggca tccccgactt ctttaagcag tccttccccg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga ccctgtaccc
      481 cgctgacggc ggcctggaag gcagagccga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aacttgaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
      601 gcccggcgtc tactatgtgg acagaagact ggaaagaatc aaggaggccg acaaagagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
      721 gcacagagac ccagctttct tgtacaaagt ggctttcagt tggtctaaca aagatggaaa
      781 aaagaaagac ccggagttat ttcaaacggt ttcggacggg ctaaagagac tgtacaggac
      841 caaacttttc cccttagagg acgcataccg ctttcacgac ttccactctc cggctctgga
      901 agatgctgat ttcgataaca agcccatggt acttttggtg ggccaatact ctacagggaa
      961 aaccactttt attcggcatc tcatggagca ggatttccct ggtatgcgca tagggcccga
     1021 accgaccacc gattcattca tagcggttat gcacggggaa caagaaggtc tcatccccgg
     1081 caatgcttta gtggttgacc ccaagaaacc cttcagaaaa ctcaacgcct ttggaaacgc
     1141 ctttctgaac aggttcatat gtgctcagat gccaaaccca gtgctggaca gcatcagcat
     1201 catagacact cctggaattc tgtccggaga gaaacaacgg atcagcagag gttatgattt
     1261 tgctgcggtg cttgaatggt ttgccgaacg ggtggaccga atcattcttc tctttgacgc
     1321 acacaagttg gacatctcgg atgagttctc cgaggtgatc cgagctctga aaaaccatga
     1381 ggacaaaatg cgggtggtgc ttaataaagc tgatcagatc gggacccagc agcttatgag
     1441 ggtgtatggc gccctcatgt ggtcacttgg gaaaattgtc aacactccag aggtcatacg
     1501 tgtgtatatt ggctctttct gggctcagcc gctcctggtt gcagataaca ggaaattgtt
     1561 tgaggcagag gaacaagacc ttttccgtga catccaggga cttccacgta atgcagcact
     1621 gcgcaaactg aatgacctta tcaaacgggc ccgtctagcc aaagttcaag catacatcat
     1681 cagctctttg aagaaagaaa tgccaaatgt gtttggaaaa gagaacaaga aaaaggagct
     1741 cattgctaac ctgggtgaaa tctatgcaaa gattgaaaaa gaacatcaga tctcccctgg
     1801 tgattttcct aaactcagta aaatgcagga agtcctcgct ggacaggact tcactaagtt
     1861 tcaaggattg aagcccaagc ttttggaatc agtggaagac atgctggcca acgacattgc
     1921 caaactcatg accctcgtcc ggcaggagga ggcctccatg ccaagccagg ctgtccaagg
     1981 tggagccttt gaaggcacca tgaatggccc cttcgggcac ggctacggag aaggtgccgg
     2041 tgaaggcatt gatgagctgg agtgggtagt ggccaaagac aaaccgacat acgacgagat
     2101 cttctacact ttgtcacccg tcaatgggaa ggtgtctggt gccatggcca agaaggagat
     2161 ggttaagtcc aagctgccca ataatgttct aggaaagatc tggaagctcg ctgacgtgga
     2221 taaggatgga tacctggatg acgaggagtt cgccctggcc aaccacctca tcaaggttaa
     2281 actggaaggt cacgagttgc cagctgatct tccagatcac cttgtgccac cttcaaaacg
     2341 agaacagtaa caactttatt atacatagtt gataattcac tggccgtcgt tttacggtac
     2401 catcgatgat gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2461 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2521 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2581 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     2641 tgattatgat cctctagatc agatctgcga agatacggcc acgggtgctc ttgatcctgt
     2701 ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc ctccattttc
     2761 ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg tgcattgtgg
     2821 taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga ccaatttcat
     2881 ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa ctgggcatca
     2941 gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat ctccaaaaaa
     3001 taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt ttttctaaaa
     3061 aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt aaagtaaaaa
     3121 tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta ctttacacct
     3181 ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct agagcggccg
     3241 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     3301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag gcattttggt catgagatta
     4381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5461 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5521 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5581 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5641 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc acgctggcta agaactcatc
     5701 agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat gacggctact
     5761 acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc aacaatgctc
     5821 ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca gaaatgccct
     5881 ctgatctgca aaagacgtga atatctgttc agacacccat atccactctg ttccacacag
     5941 gtcagaggtt tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc
     6001 aagtgaaagt acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct
     6061 agaatcttac ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag
     6121 gaagtaaaag taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa
     6181 aaaacattaa atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat
     6241 tatcaaacta taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct
     6301 cagatttaac ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt
     6361 ggggctgaaa agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag
     6421 aggtgtatta gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct
     6481 ggtgtctgaa acacaggcca gatgggccct cgagcaggaa acagctatga ccatgattac
     6541 gccaagctat caactttgta tagaaaagtt ggcacgcgca gattccgtgt gggcctggta
     6601 atatcttacc attttaagct attttaagat tagggaaaaa aattaaataa aactacttta
     6661 aaattacttt ccttaaaaag caacatattt atttagtttt tgtttaactg gctcaagatt
     6721 gtaatatttt acttttacat gtttttttaa gtacctaaat gtgttcaaaa gtaaagtatg
     6781 ttattgtttt ttttttttaa tgttagctaa gctttaaatt gtaatatatt acttttaaaa
     6841 gtgacttttt aaaaatgtaa ataaaaaagt aactataaat gtgtaatgca tgttatttct
     6901 gtgggttttt aattttcatc tcaactgggc ttacaatttt aacataataa aaatcaaatc
     6961 aaatcaaatc aggcccttat ttgtcacata cactcttgta tactgaaata aactaaatgt
     7021 tgagaaaaat ttaaatacac caaaataacg catacctttt ttgtaattac ctaagtaaaa
     7081 catttagttc cttttttaag tgactcaaga ttgtaatcta ttaattgtaa aagataaaac
     7141 tagtgatcaa tctgacgtgc ttctatgcag cctgtgagat taattgtgat gtttaattat
     7201 taaacaattt aaatggatca aacatttcac atgtgcttat tgtgtccaac aaaaccagca
     7261 gaagcactta aaaatattgc atgacaacat gagtttttaa tgtaaattat atatacttcc
     7321 taaacgaagt taatcatatt tctaaaataa taatgtcatt gaaatattta aataaatgat
     7381 aagctttagt tgattcaaca taaaattgaa agcaataagg actttttttt tttacagcgg
     7441 tgaagcaaaa gcatgtaata atcatataat ataataaata aataaataaa taatggctct
     7501 tggtgcaaat atgaacactt taaataaata tagaataaat atgatcaata agtcataacg
     7561 atgtgtttta tgttgattat aatttaaaca tgtttctaac gcaagtttta aaaactgttt
     7621 taaagtatta ttttaagtta taagatcaat ttgactcaac attttgtagt gaaaatatgt
     7681 tgtgtaaaat taatataata tacactataa aaatgcaggg ttccacacaa tacattaatg
     7741 ttgtcccaac acaaatcgat taagttaaca aatatatgtg gattgaacat aaaaaattaa
     7801 gttgtcccaa tgaaatctca agaattgtgt tgtttcagct cattttaaat aagtagtttt
     7861 aacaagtaaa aaataacata tttttgagtg tgtatatata tatattacaa aatataataa
     7921 tataaaatgt aaaagatata aaccatgcta catatattta agagctattt tgcacattac
     7981 ttttttgcaa gccattttag tgtttttttt acaaatatat attagctaaa tatacaatat
     8041 cttaaatata caattaatca gacatgcaat taatgcattt aacctacata aaaatacatg
     8101 atcaacatat gttttatgct gattacaact aaatcaaacg taatgaaatt aatttataaa
     8161 agcgatttaa atatgtcaat attttaagtt aaaatcactt gaaacctact ataaatgaac
     8221 ttaaattaaa aaaatctcct ttaattccaa tgcaacagtg ttgttttttc agctgaggac
     8281 ccccggtgca gatggttggc ccccggcccg ctattctaag gaagcctttc ttaattttag
     8341 ggcggcgctg catgcgctca gatcagattc acgggtggag agggttattt tctctttttt
     8401 attttttctt gcctgctcat atttgggcct gtgtgaaccc caggctccgc ctcggccccc
     8461 aggagccgct cttatttaaa ggggatcctc gccttcagtc ttcgtcccag tggaagcatc
     8521 agtcttgtga taacaacttg gtgagtccta cagaccagcg ctcgactcta ggctttaaat
     8581 gcattgattt ctctggatta gctaccggga gagttttaac tttgcttatt agtcttgtgc
     8641 tcgaactgct aggtttaaag agtgttttct ctaatgcatg catgaagcga cagctgaaaa
     8701 ccagaaatag agcgtttgtc ctcctgttaa actctagcct ttctttggca tttgtcagtc
     8761 cgagtcttca gctgccggca ggagattggg tttcagtaac acttgctgag cacaaactac
     8821 acgtgctttc ttcagattta attcattatt aatcattcta gatttaaatg atgttcttac
     8881 tcattgctga ttattttagc cttgcggagc agattaaatg agcacgtgtt tcattaagat
     8941 tgatttaaat ataattaata tttcaccact gtagttttaa aattgccttc taagtcatgg
     9001 ttgattttta ataggtatcg gctctaatta gattactatt ttggttttta aatgttattt
     9061 ctatttcatt tgtaggctcg tttttaatgt gattttatta gattctagtt ttaaaatcat
     9121 ctcttagcct taatgagcag actgaatgaa catgtttcat taagattaac ttcattataa
     9181 tgaattattt aatcattcta gtgggttata aatcagctct atttaaatta attgtcgtgt
     9241 ttatttctat ttaatttttg attatttttt cagggtcgtt tttatgtgat tttttcccgt
     9301 ttctaatttt aaactaattg tttgattttt atatgattcg actcttttta aatgaattgt
     9361 tttgcttttt ttattgagca tatatatatt atacaatttc aatcaacaat tgttgtgctt
     9421 tttaaatgtt gtttctattt catttctagg gtcgatttta ttcgattcta gttttaaaat
     9481 cgtgattttt aacttctaac cttactgagc agactgaatg aacatgtttc atcaagatta
     9541 acttcattat agttaattat tgaatcgtgc tagtggttta taaatcagct ctatttaaat
     9601 taatcatcat cattatttct atttaatttt tattatttct ttaggattat ttttatgtga
     9661 tttttttcac gttctagttt taaactcaat gctcattact gctcaccctt gctgagcagt
     9721 ctttcgttaa gattaaatta attaataacc attctagttt taaaacagtg tcttaaggca
     9781 tagctgatat tttacaagtt tactatttaa tatatctcct ttcttttttt agggtcgatt
     9841 tcatctgatt tgattaattt ttattatctt cactaagctt tttgttctag gttttattct
     9901 cagtattatt actgttatat ttgaactgaa tttctcctca tagtttattc attttattta
     9961 tttatttttt gtttattcgc tattttgtat tttagttttt aattagaaat tttattagaa
    10021 tcgttttgaa ctagttttta tgcactctaa cattaatatt aaatcataac tattattttt
    10081 attataattt atttgtgttt atctttttgt gtagctttca tgtgttttta ttttagtttt
    10141 attatgtttg cattgaattt tttggactag tttatataac gatatacaga aaagccatga
    10201 caacaaatgt ttttataata cctattaagt ataagctaat cagatttcca ctattctgtt
    10261 caaagttaac attattagcc attctgatgg atcatttgac atttgtcata tgtctttatg
    10321 aacactgctc tttcagatgc cacttttaaa gctgtttttt acagtaaatg aacgtttatt
    10381 attatgacat tatttcctaa ggcctctaga gtacagcagg atgtggcgtg tgcattaccg
    10441 tgtaattatg ctgactgtaa atgtgcattt atagactgtc agcatgtctg actgtgcgct
    10501 gttgtgattg gtcctgcaca gaccaag
//
