LOCUS       Exported                5559 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    pUG-UBC23
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     211..228
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3654..3672)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3740..3844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4066..4857
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5028..57
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5517..5536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      241 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
      301 ctcaaatgga gcatgagcaa gatgaccctg gtacatcaac aaatgtaggg gttgactcgt
      361 cagttgatga ttcaatggca agtctctcta tatgtgattc agagcatcct aatatttatc
      421 gacaagatat tgtcaaaaac aaaaagaccg gtagtgttgg tgttgtcagc gaagtagctg
      481 gtgattctga ctctgatagt gatattagtg atgaggaaga agatgatgat gatgacgaag
      541 ataatgatga tgatgatgag gatgtagagg aagggaaaaa ggctagtgaa gaaaatgttg
      601 ttaatggaga tggcgagaaa aaagctgatg ggaattataa atgtggtgca cttgagggag
      661 atcagattcg tgtgctttgg atggataaca ctgagcctgt tcaagatatt aatgatgtaa
      721 ctgttataga ccgtggtttc ttacatggtg attatgttgc ttctgcttct gagcctactg
      781 gtcaggtggg agttgttgtg gatgttaaca tatcagttga tttgttggct cctgatggtt
      841 ctattcacaa ggacatctct accaagaatt tgaaacgcgt cagggatttt gctgttggtg
      901 attatgtggt tcatggtcca tggctaggta gaatcgatga tgtgttggac aatgtgactg
      961 tcttatttga tgatggttcc atgtgtaaag ttctacgtgt tgaacctctt cgacttaaac
     1021 ctattccgaa gaataatctt gaagaagatg ctaattttcc ttattaccca gggcagcggg
     1081 ttaaagcaag ctcctcttcg gtttttaaga attcgcggtg gttatctgga ctgtggaagc
     1141 ctaatcgttt agaaggtacg gtgaccaaag ttactgcggg atcaattttt gtctactgga
     1201 ttgcctcagc tggctttggt ccagattctt ctgtttcccc acctgaggag cagaatccaa
     1261 gtaatttgac attgttgtca tgtttcactc atgccaattg gcaagtgggt gactggtgcc
     1321 ttcttccatc tctgaatcaa tctgctacaa tccccttaca caagcatgta tctaaattaa
     1381 gactttatga ttcccaagct gatcggcaac agaaaattgg gcgtgattta gaagatgtgc
     1441 aagatgaagt aagtggaaaa gttgaaccag ctggtattac tgctgaagct ttgccaaaag
     1501 taactagtga cgatcctcct caaagaaatc cttctgtttc aaaggagcct gttcatgagc
     1561 cttggcctct ccatcggaag aagattcgca aacttgttat cagaaaggac aaaaaggtta
     1621 agaaaaaaga ggaaagcttt gaacaagctc ttttggtagt taatagcaga acacgcgttg
     1681 atgtatcctg gcaggatggt acaatagaat gtagacggga agcgataaca ttgattccaa
     1741 ttgagactcc tggtgatcat gaatttgtct ctgagcagta tgtggtagag aggacctctg
     1801 atgatggtga taacacaact gaacctagac gtgctggcgt tgtaaaaaat gtcaatgcaa
     1861 aagatcgtac tgcttctgtg aggtggctaa atccacttcg aagggcagaa gaaccccgtg
     1921 agtttgaaaa ggaagaaatt gtcagtgtgt atgagctaga gggccatcca gattatgact
     1981 actgttatgg ggatgttgtt gttcgcttat cacctattgc cgttgcgtta ccagcatctt
     2041 ctcccggaaa ctcttttgag gaggcaacac agcaagacaa tggataccaa gattcagaat
     2101 cccatcaaga agctaaaatc cttgtggata aggaagaaaa cgagcctagt acggaccttt
     2161 caaagctctc atgggttgga aatattactg gcctcaagga tggtgatatt gaagtaacat
     2221 gggccgatgg aacaatatca acggttggcc ctcatgcagt ttatgtagtt ggacgcgatg
     2281 atgatgatga atcagttgct ggagaaagtg aaacaagtga tgctgctagt tgggaaactt
     2341 taaacgatga tgataggggt gctcctgaga ttcctgaaga ggaccttgga aggagtagtt
     2401 ccattgaggg aaactctgat gcagatatct acgctgagaa tgattctggg aggaacggtg
     2461 ccctagctct tcctctggct gcaatcgaat ttgtgactcg actggctagt ggaatctttt
     2521 cgcgtgcaag gaaatctgta gattcttcca gttctgatta cacggttgaa aatgtatata
     2581 agcaagctga atcgaccaac ccgtccgatg aaacagattc ccttgatgac cctagtcctt
     2641 caaaagttaa tgtgactgat aattgcgagt caaagggcac tcaagcaaat gccaagaata
     2701 ttttgagcgg ggaaacctct acatttttag aagatgaaga taagccagta ccttctgaag
     2761 gtgatagttg cagtttcaga cgctttgata tatcacaaga tcctctggac caccatttcc
     2821 ttggcgtaga tgggcagaaa accaaagaaa gacagtggtt caagaaggtt gatcaagact
     2881 ggaaaatact tcagaacaat cttccagatg gaatctttgt tcgagcttac gaagatagaa
     2941 tggatctctt gagggcagta atagtcgggg catttggaac accataccaa gatggactct
     3001 tcttttttga ttttcacctt ccatctgact accctagtgt gccaccgtcg gcatattatc
     3061 attctggtgg atggaggtta aaccccaatc tgtacgaaga aggcaaagtc tgtcttagcc
     3121 ttctcaatac ctggacaggc aggggaaatg aagtctggga tcccaaatca tctagcatcc
     3181 ttcaagttct tgtttcactc cagggattgg tgttgaattc aaagccttat ttcaatgaag
     3241 ccgggtatga taagcaggtc ggaactgcgg aaggagaaaa aaactcactg ggatacaacg
     3301 agaatacttt cttgctaaat tgtaaaacca tgatgtatct tatgcggaaa ccaccaaagg
     3361 attttgaaga actcatcaaa gatcatttca gaaaacgcgg atattacatt ttgaaggcat
     3421 gtgacgccta catgaaagga tatctcatag gttccctcac caaagatgcc tcggttatcg
     3481 atgaacgcag cagcgctaat tcaacttcag ttggttttaa gcttatgctg gctaagatag
     3541 caccaaagct tttctcagca ctgagcgagg taggagctga ctgcaatgaa ttccagcatc
     3601 tgcagcagca ataagctttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt
     3661 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3841 gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     3901 agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg
     3961 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     4021 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
     4081 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     4141 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     4201 agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     4261 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     4321 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     4381 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     4441 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     4501 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     4561 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     4621 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     4681 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     4741 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     4801 ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg
     4861 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4921 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4981 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5041 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     5101 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5161 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5221 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5281 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5341 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5401 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5461 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5521 aacgcctggt atctttatag tcctgtcggg tttcgccac
//
