LOCUS Exported 3912 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cloning Module. ACCESSION . VERSION . KEYWORDS pUG-233 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3912) AUTHORS Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M TITLE UbiGate: a synthetic biology toolbox to analyse ubiquitination. JOURNAL New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 2017 Nov 30. PUBMED 29194629 REFERENCE 2 (bases 1 to 3912) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3912 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 174..191 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(1970..1988) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2056..2160 /gene="bla" /label=AmpR promoter CDS 2382..3173 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3344..20 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3833..3852 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 61 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 121 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 181 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 241 cgattcatta atcactctgt ggtctcaaat ggcgggtatc aatagagcgg gaccatcggg 301 cgcttatttt gttggccaca cagaccccga gccagtatcg gggcaagcac acggatccgg 361 cagcggcgcc agctcctcga acagtccgca ggttcagccg cgaccctcga atactccccc 421 gtcgaacgcg cccgcaccgc cgccaaccgg acgtgagagg ctttcacgat ccacggcgct 481 gtcgcgccaa accagggagt ggctggagca gggtatgcct acagcggagg atgccagcgt 541 gcgtcgtagg ccacaggtga ctgccgatgc cgcaacgccg cgtgcagagg caagacgcac 601 gccggaggca actgccgatg ccagcgcacc gcgtagaggg gcggttgcac acgccaacag 661 tatcgttcag caattggtca gtgagggcgc tgatatttcg catactcgta acatgctccg 721 caatgcaatg aatggcgacg cagtcgcttt ttctcgagta gaacagaaca tatttcgcca 781 gcatttcccg aacatgccca tgcatggaat cagccgagat tcggaactcg ctatcgagct 841 ccgtggagcg cttcgtcgag cggttcacca acaggcggcg tcagcgccag tgaggtcgcc 901 cacgccaaca ccggccagcc ctgcggcatc atcatcgggc agcagtcagc gttctttatt 961 tggacggttt gcccgtttga tggcgccaaa ccagggacgg tcgtcgaaca ctgccgcctc 1021 tcagacgccg gtcgacagga gcccgccacg cgtcaaccaa agacccatac gcgtcgacag 1081 ggctgcgatg cgtaatcgtg gcaatgacga ggcggacgcc gcgctgcggg ggttagtaca 1141 acagggggtc aatttagagc acctgcgcac ggcccttgaa agacatgtaa tgcagcgcct 1201 ccctatcccc ctcgatatag gcagcgcgtt gcagaatgtg ggaattaacc caagtatcga 1261 cttgggggaa agccttgtgc aacatcccct gctgaatttg aatgtagcgt tgaatcgcat 1321 gctggggctg cgtcccagcg ctgaaagagc gcctcgtcca gccgtccccg tggctcccgc 1381 gaccgcctcc aggcgaccgg atggtacgcg tgcaacacga ttgcgggtga tgccggagcg 1441 ggaggattac gaaaataatg tggcctatgg agtgcgcttg cttaacctga acccgggggt 1501 gggggtaagg caggctgttg cggcctttgt aaccgaccgg gctgagcggc cagcagtggt 1561 ggctaatatc cgggcagccc tggaccctat cgcgtcacaa ttcagtcagc tgcgcacaat 1621 ttcgaaggcc gatgctgaat ctgaagagct gggttttaag gatgcggcag atcatcacac 1681 ggatgacgtg acgcactgtc tttttggcgg agaattgtcg ctgagtaatc cggatcagca 1741 ggtgatcggt ttggcgggta atccgacgga cacgtcgcag ccttacagcc aagagggaaa 1801 taaggacctg gcgttcatgg atatgaaaaa acttgcccaa ttcctcgcag gcaagcctga 1861 gcatccgatg accagagaaa cgcttaacgc cgaaaatatc gccaagtatg cttttagaat 1921 agtcccctga gctttgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata 1981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 2041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 2101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 2161 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg 2221 gcggttttca tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat 2281 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 2341 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg 2401 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 2461 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg 2521 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 2581 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 2641 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 2701 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 2761 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 2821 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 2881 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 2941 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 3001 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 3061 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 3121 aagaatttgt ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag 3181 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3361 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3421 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3481 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 3541 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3601 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3661 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3721 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3781 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3841 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3901 gatgctcgtc ag //