LOCUS       Exported                 762 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  expresssion of gRNA targeting POMT2.
ACCESSION   .
VERSION     .
KEYWORDS    gh24
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen YH, Lorenzetti F, 
            Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall 
            HH
  TITLE     A validated gRNA library for CRISPR/Cas9 targeting of the human 
            glycosyltransferase genome.
  JOURNAL   Glycobiology. 2018 Jan 5. pii: 4791732. doi: 10.1093/glycob/cwx101.
  PUBMED    29315387
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     106..125
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        205..280
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        377..662
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
       61 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      121 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      181 accgcttcga ggcggtcggc tggtgtttta gagctagaaa tagcaagtta aaataaggct
      241 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
      301 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcgccaattc tgcagacaaa
      361 tggctctaga ggtacccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      421 acgacccccg cccattgacg tcaatagtaa cgccaatagg gactttccat tgacgtcaat
      481 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      541 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattgt gcccagtaca
      601 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      661 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      721 caattttgta tttatttatt ttttaattat tttgtgcagc ga
//
