LOCUS Exported 6072 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mCherry Control. ACCESSION . VERSION . KEYWORDS pAAV-CaMKIIa-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6072) TITLE mCherry Control JOURNAL Unpublished REFERENCE 2 (bases 1 to 6072) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6072 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 149..1437 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" regulatory 1458..1467 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1464..2174 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1609..1627) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1877..1896 /label=mCherry-F /note="mCherry, forward primer" misc_feature 2199..2787 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2252..2272) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2670..2681) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2819..3295 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(3018..3037) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3335..3475 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3335..3464 /label=AAV2 ITR rep_origin 3550..4005 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3637..3656) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3847..3868 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4022..4041) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4141..4163 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4201..4219) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4287..4391 /gene="bla" /label=AmpR promoter CDS 4392..5252 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4610..4629) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5423..6011 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5912..5931 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg 181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc 241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa 301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag 361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca 421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt 481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc 541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca 601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt 661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg 721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag 781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt 841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca 901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg 961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt 1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg 1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag 1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa 1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa 1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc 1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt 1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct 1441 agagcggtac cggatccgcc accatggtga gcaagggcga ggaggataac atggccatca 1501 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 1561 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 1621 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 1681 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 1741 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 1801 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 1861 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 1921 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 1981 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 2041 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 2101 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 2161 agctgtacaa gtaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa 2221 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 2281 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 2341 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 2401 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggaatt gccaccacct 2461 gtcagctcct ttccggaact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 2521 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 2581 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc 2641 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 2701 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 2761 ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg 2821 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt 2881 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt 2941 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac 3001 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct 3061 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt 3121 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg 3181 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg 3241 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat 3301 tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc 3361 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc 3421 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc 3481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc 3541 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 3601 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct 3661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 3721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac 3781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 3841 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt 3901 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac 3961 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca 4021 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 4081 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 4141 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 4201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 4261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 4381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 4441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 4501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 4561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 4621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 4681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 4741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 4801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 4861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 4921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 4981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 5041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 5101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 5161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 5221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 5281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 5341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 5401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 5461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 5521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt 5581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 5641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 5701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 5761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 5821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 5881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 5941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 6001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 6061 ttgctcacat gt //