LOCUS Exported 6030 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mCherry Control. ACCESSION . VERSION . KEYWORDS pAAV-Ef1a-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6030) TITLE mCherry Control JOURNAL Unpublished REFERENCE 2 (bases 1 to 6030) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6030 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 1..130 /label=AAV2 ITR (alternate) /note="Functional equivalent of wild-type AAV2 ITR" promoter 231..1409 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 462..1400 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1357..1377 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 1416..1425 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1422..2132 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1567..1585) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1835..1854 /label=mCherry-F /note="mCherry, forward primer" misc_feature 2157..2745 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2210..2230) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(2628..2639) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" polyA_signal 2777..3253 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(2976..2995) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" repeat_region 3293..3433 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 3293..3422 /label=AAV2 ITR rep_origin 3508..3963 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3595..3614) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3805..3826 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3980..3999) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4099..4121 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4159..4177) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4245..4349 /gene="bla" /label=AmpR promoter CDS 4350..5210 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4568..4587) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5381..5969 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5870..5889 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgcac gcgtaagctt tgcaaagatg gataaagttt taaacagaga 181 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 241 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 301 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 361 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 421 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 481 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 541 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 601 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 661 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 721 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 781 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 841 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 901 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 961 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1021 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1081 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1141 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1201 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1261 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1321 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1381 gtttttttct tccatttcag gtgtcgtgag gatccgccac catggtgagc aagggcgagg 1441 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 1501 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 1561 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 1621 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 1681 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 1741 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 1801 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 1861 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 1921 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 1981 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 2041 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 2101 ccaccggcgg catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataatc 2161 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 2221 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 2281 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 2341 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 2401 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 2461 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 2521 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 2581 atgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 2641 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 2701 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc 2761 tgctcgagag atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct 2821 ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt 2881 gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg 2941 gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc 3001 agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc 3061 tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt 3121 tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca 3181 ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc 3241 cttccctgtc cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc 3301 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga 3361 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc 3421 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca 3481 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 3541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt 3601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 3661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 3721 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 3781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact 3841 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa 3901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 3961 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 4021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 4081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 4141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 4201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 4261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 4321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 4381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 4441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 4501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 4561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 4621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 4681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 4741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 4801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 4861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 4921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 4981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 5041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 5101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 5161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 5221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 5281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 5341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 5401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 5461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 5521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 5581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 5641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 5701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 5761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 5821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 5881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 5941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 6001 tggccttttg ctggcctttt gctcacatgt //