LOCUS Exported 4771 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION dark ITRACK acceptor construct for dual color imaging. ACCESSION . VERSION . KEYWORDS CMV-ShadowY-kCAAX SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4771) AUTHORS Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R TITLE PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. JOURNAL Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. PUBMED 30013171 REFERENCE 2 (bases 1 to 4771) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4771 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 607..616 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(658..679) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(919..938) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1266..1287 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1559..1680 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1596..1615) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1650..1669 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1687..2142) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1824..1845) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2036..2055 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2169..2273 /gene="bla" /label=AmpR promoter promoter 2275..2632 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2298..2318) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2483..2618 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2545..2564 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2667..3461 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2721..2740) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3331..3350 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3649..3668) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3693..3740 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4069..4657 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4558..4577 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ctcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccttcggc tacggcctga tgtgcttcgc gcgctatcca 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctcga gtacgcttgg cccgtggtga atgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc tctatctatc ataacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacgggcc cgtgctgctg 1201 cccgacaacc actacctgag ctactcggct aagctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtaaa 1381 aagaagaaaa agaagtcaaa gacaaagtgt gtaattatgt aagggcccgg gatccaccgg 1441 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 1501 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 1561 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 1621 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 1681 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 1741 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 1801 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 1861 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 1921 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 1981 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 2041 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 2101 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 2161 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 2221 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 2281 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 2341 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 2401 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 2461 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 2521 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 2581 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 2641 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 2701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 2761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 2821 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 2881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 2941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 3001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 3061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 3121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 3181 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 3241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 3301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 3361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 3421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 3481 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 3541 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 3601 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 3661 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 3721 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 3781 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 3841 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 3901 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 3961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 4021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 4081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 4141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 4201 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 4261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 4321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 4381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 4441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 4501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 4561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 4621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 4681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 4741 ttctgtggat aaccgtatta ccgccatgca t //