LOCUS       Exported               11409 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial expression of Cas9 nuclease and lambda-Red system in 
            Klebsiella Pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    pCasKP-apr
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
  TITLE     Precise and efficient genome editing in Klebsiella pneumoniae using 
            CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
  JOURNAL   Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 
            10.1128/AEM.01834-18.
  PUBMED    30217854
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        1867..1969
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2036..2055)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2425..3225
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(3455..4333)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4360..4644
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4567..4586
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4703..5119
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             5125..5910
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             5907..6587
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             7169..11275
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg
     1381 accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct
     1441 ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa
     1501 ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg
     1561 ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc
     1621 tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc
     1681 agcggtatca tcaacaggct tacccgtctt actgtcgggg atcgacgctc tcccttatgc
     1741 gactcctgca cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata
     1801 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
     1861 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     1921 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1981 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2041 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctgggtc atccaccgga
     2101 tcaattcccc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc
     2161 cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac
     2221 ccgcagttgc aaaccctcac tgatccggct cacggtaact gatgccgtat ttgcagtacc
     2281 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     2341 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     2401 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac
     2461 gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt
     2521 gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga
     2581 ctgatcgagg ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat gccctcgtgg
     2641 tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga
     2701 gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc
     2761 tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct
     2821 cactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc
     2881 ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc
     2941 tatggggtgc cgagacactg caccattctt caggatggca agttggtacg cgtcgattat
     3001 ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag
     3061 agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac
     3121 attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca
     3181 gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg
     3241 agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgtgaaagg
     3301 cgagatcacc aaggtagtcg gcaaataatg tctaacggta cccggggatc ctctagagtc
     3361 gacctgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact
     3421 ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat
     3481 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa
     3541 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag
     3601 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc
     3661 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc
     3721 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt
     3781 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt
     3841 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc
     3901 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg
     3961 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat
     4021 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat
     4081 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa
     4141 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat
     4201 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg
     4261 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt
     4321 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt
     4381 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa
     4441 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta
     4501 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac
     4561 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat
     4621 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagcgaaaag atgtttcgtg
     4681 aagccgtcga cgcttataaa aaatggatat taatactgaa actgagatca agcaaaagca
     4741 ttcactaacc ccctttcctg ttttcctaat cagcccggca tttcgcgggc gatattttca
     4801 cagctatttc aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca
     4861 gagctgggcg cgtcactacc agcagctcgc ccgtgaagag aaagaggcag aactggcaga
     4921 cgacatggaa aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca
     4981 acgccacggg gccagcaaaa aatccattac ccgtgcgttt gatgacgatg ttgagtttca
     5041 ggagcgcatg gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga
     5101 tattgattca gaggtataaa acgaatgagt actgcactcg caacgctggc tgggaagctg
     5161 gctgaacgtg tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag
     5221 acggcattta aaggtgatgc cagcgatgcg cagttcatcg cattactgat cgttgccaac
     5281 cagtacggcc ttaatccgtg gacgaaagaa atttacgcct ttcctgataa gcagaatggc
     5341 atcgttccgg tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt
     5401 gatggcatgg actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac
     5461 cgtaatcatc cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa
     5521 actcgcgaag gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgt
     5581 cataaagcca tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag
     5641 gatgaagccg agcgcattgt cgaaaatact gcatacactg cagaacgtca gccggaacgc
     5701 gacatcactc cggttaacga tgaaaccatg caggagatta acactctgct gatcgccctg
     5761 gataaaacat gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt
     5821 cgtgcatcgt cagaactgac acaggccgaa gcagtaaaag ctcttggatt cctgaaacag
     5881 aaagccgcag agcagaaggt ggcagcatga caccggacat tatcctgcag cgtaccggga
     5941 tcgatgtgag agctgtcgaa cagggggatg atgcgtggca caaattacgg ctcggcgtca
     6001 tcaccgcttc agaagttcac aacgtgatag caaaaccccg ctccggaaag aagtggcctg
     6061 acatgaaaat gtcctacttc cacaccctgc ttgctgaggt ttgcaccggt gtggctccgg
     6121 aagttaacgc taaagcactg gcctggggaa aacagtacga gaacgacgcc agaaccctgt
     6181 ttgaattcac ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta
     6241 tgcgtaccgc ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat
     6301 gcccgtttac ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaagt
     6361 cagcttacat ggcccaggtg cagtacagca tgtgggtgac gcgaaaaaat gcctggtact
     6421 ttgccaacta tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcggg
     6481 atgaaaagta catggcgagt tttgacgaga tcgtgccgga gttcatcgaa aaaatggacg
     6541 aggcactggc tgaaattggt tttgtatttg gggagcaatg gcgatgacgc atcctcacga
     6601 taataagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa gacagcatcc
     6661 ttgaacaagg acaattaaca gttaacaaat aaaaacgcaa aagaaaatgc cgatattgac
     6721 taccggaagc agtgtgaccg tgtgcttctc aaatgcctga ttcaggctgt ctatgtgtga
     6781 ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt
     6841 actggtttca cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg
     6901 ttcatggtga acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt
     6961 ttttacaccg ttttcatctg tgcatatagc tatactgatt tcgtcagact cacagtcaaa
     7021 catgccggtc agttggcctg gtgatggcgg gatcgttgta tatttcttga caccttttcg
     7081 gcaccgccct aaaattctgc gtcctcataa tatatgaggc gatttattac gtgtttacga
     7141 agcaaaagct aaaaccagga gctatttaat ggataagaaa tactcaatag gcttagatat
     7201 cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa
     7261 gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct
     7321 tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag
     7381 gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc
     7441 gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa
     7501 gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa
     7561 atatccaact atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt
     7621 gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga
     7681 gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac
     7741 ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat
     7801 tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg
     7861 tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa
     7921 ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta
     7981 cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg atttgttttt
     8041 ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag taaatactga
     8101 aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga
     8161 cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt
     8221 ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga
     8281 attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt
     8341 gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc
     8401 ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc
     8461 atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta
     8521 tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga
     8581 aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt
     8641 tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca
     8701 tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac
     8761 tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt
     8821 actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa
     8881 aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt
     8941 aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga
     9001 aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat
     9061 gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct
     9121 taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag
     9181 ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg
     9241 caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc
     9301 acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc
     9361 tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat
     9421 ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca
     9481 aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt
     9541 aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct
     9601 ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa
     9661 tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta aagacgattc
     9721 aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc
     9781 aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt
     9841 aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact
     9901 tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt
     9961 ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg
    10021 agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca
    10081 attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc
    10141 cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg
    10201 tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa
    10261 agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac
    10321 acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga
    10381 aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca
    10441 agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt
    10501 accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata
    10561 tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa
    10621 agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag
    10681 aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa
    10741 aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa
    10801 acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa
    10861 atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga
    10921 taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga
    10981 gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct
    11041 tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca
    11101 tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat
    11161 tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc
    11221 catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actgatggct
    11281 ggttggcgta ctgttgtggt aaatacccat atggacagtt ttccctttga tatgtaacgg
    11341 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
    11401 ataagaacc
//
