LOCUS       Exported               11631 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Bacterial expression of Cas9 nuclease and lambda-Red system in 
            Klebsiella Pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    pCasKP-hph
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11631)
  AUTHORS   Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
  TITLE     Precise and efficient genome editing in Klebsiella pneumoniae using 
            CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
  JOURNAL   Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 
            10.1128/AEM.01834-18.
  PUBMED    30217854
REFERENCE   2  (bases 1 to 11631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(1..951)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      999..1221
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     promoter        1867..1969
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(2036..2055)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(3677..4555)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4582..4866
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4789..4808
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4925..5341
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             5347..6132
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             6129..6809
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             7391..11497
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
       61 ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc
      121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
      481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
      541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
      601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
      661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
      721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
      781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
      841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
      901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
      961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     1321 cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct tttgtctccg
     1381 accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct
     1441 ctggcagtga atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa
     1501 ctacccataa tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg
     1561 ctatgtggtg ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc
     1621 tgaccacttc ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc
     1681 agcggtatca tcaacaggct tacccgtctt actgtcgggg atcgacgctc tcccttatgc
     1741 gactcctgca cctttcgtct tcgaataaat acctgtgacg gaagatcact tcgcagaata
     1801 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg
     1861 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc
     1921 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa
     1981 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
     2041 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctgggtc atccaccgga
     2101 tcaattcccc tgctcgcgca ggctgggtgc caagctctcg ggtaacatca aggcccgatc
     2161 cttggagccc ttgccctccc gcacgatgat cgtgccgtga tcgaaatcca gatccttgac
     2221 ccgcagttgc aaaccctcac tgatccggct cacggtaact gatgccgtat ttgcagtacc
     2281 agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt
     2341 gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc
     2401 cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt catgacacaa
     2461 gaatccctgt tacttctcga ccgtattgat tcggatgatt cctacgcgag cctgcggaac
     2521 gaccaggaat tctgggagcc gctggcccgc cgagccctgg aggagctcgg gctgccggtg
     2581 ccgccggtgc tgcgggtgcc cggcgagagc accaaccccg tactggtcgg cgagcccgac
     2641 ccggtgatca agctgttcgg cgagcactgg tgcggtccgg agagcctcgc gtcggagtcg
     2701 gaggcgtacg cggtcctggc ggacgccccg gtgccggtgc cccgcctcct cggccgcggc
     2761 gagctgcggc ccggcaccgg agcctggccg tggccctacc tggtgatgag ccggatgacc
     2821 ggcaccacct ggcggtccgc gatggacggc acgaccgacc ggaacgcgct gctcgccctg
     2881 gcccgcgaac tcggccgggt gctcggccgg ctgcacaggg tgccgctgac cgggaacacc
     2941 gtgctcaccc cccattccga ggtcttcccg gaactgctgc gggaacgccg cgcggcgacc
     3001 gtcgaggacc accgcgggtg gggctacctc tcgccccggc tgctggaccg cctggaggac
     3061 tggctgccgg acgtggacac gctgctggcc ggccgcgaac cccggttcgt ccacggcgac
     3121 ctgcacggga ccaacatctt cgtggacctg gccgcgaccg aggtcaccgg gatcgtcgac
     3181 ttcaccgacg tctatgcggg agactcccgc tacagcctgg tgcaactgca tctcaacgcc
     3241 ttccggggcg accgcgagat cctggccgcg ctgctcgacg gggcgcagtg gaagcggacc
     3301 gaggacttcg cccgcgaact gctcgccttc accttcctgc acgacttcga ggtgttcgag
     3361 gagaccccgc tggatctctc cggcttcacc gatccggagg aactggcgca gttcctctgg
     3421 gggccgccgg acaccgcccc cggcgcctga gctcatgagc ggagaacgag atgacgttgg
     3481 aggggcaagg tcgcgctgat tgctggggca acacgtgaaa ggcgagatca ccaaggtagt
     3541 cggcaaataa tgtctaacgg tacccgggga tcctctagag tcgacctgca taatgtgcct
     3601 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg
     3661 tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac
     3721 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga
     3781 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
     3841 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
     3901 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
     3961 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
     4021 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
     4081 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
     4141 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
     4201 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
     4261 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
     4321 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
     4381 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
     4441 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
     4501 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
     4561 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
     4621 actgcgtctt ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca
     4681 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
     4741 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
     4801 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
     4861 ctccataccc gtttttttgg gctagcgaaa agatgtttcg tgaagccgtc gacgcttata
     4921 aaaaatggat attaatactg aaactgagat caagcaaaag cattcactaa ccccctttcc
     4981 tgttttccta atcagcccgg catttcgcgg gcgatatttt cacagctatt tcaggagttc
     5041 agccatgaac gcttattaca ttcaggatcg tcttgaggct cagagctggg cgcgtcacta
     5101 ccagcagctc gcccgtgaag agaaagaggc agaactggca gacgacatgg aaaaaggcct
     5161 gccccagcac ctgtttgaat cgctatgcat cgatcatttg caacgccacg gggccagcaa
     5221 aaaatccatt acccgtgcgt ttgatgacga tgttgagttt caggagcgca tggcagaaca
     5281 catccggtac atggttgaaa ccattgctca ccaccaggtt gatattgatt cagaggtata
     5341 aaacgaatga gtactgcact cgcaacgctg gctgggaagc tggctgaacg tgtcggcatg
     5401 gattctgtcg acccacagga actgatcacc actcttcgcc agacggcatt taaaggtgat
     5461 gccagcgatg cgcagttcat cgcattactg atcgttgcca accagtacgg ccttaatccg
     5521 tggacgaaag aaatttacgc ctttcctgat aagcagaatg gcatcgttcc ggtggtgggc
     5581 gttgatggct ggtcccgcat catcaatgaa aaccagcagt ttgatggcat ggactttgag
     5641 caggacaatg aatcctgtac atgccggatt taccgcaagg accgtaatca tccgatctgc
     5701 gttaccgaat ggatggatga atgccgccgc gaaccattca aaactcgcga aggcagagaa
     5761 atcacggggc cgtggcagtc gcatcccaaa cggatgttac gtcataaagc catgattcag
     5821 tgtgcccgtc tggccttcgg atttgctggt atctatgaca aggatgaagc cgagcgcatt
     5881 gtcgaaaata ctgcatacac tgcagaacgt cagccggaac gcgacatcac tccggttaac
     5941 gatgaaacca tgcaggagat taacactctg ctgatcgccc tggataaaac atgggatgac
     6001 gacttattgc cgctctgttc ccagatattt cgccgcgaca ttcgtgcatc gtcagaactg
     6061 acacaggccg aagcagtaaa agctcttgga ttcctgaaac agaaagccgc agagcagaag
     6121 gtggcagcat gacaccggac attatcctgc agcgtaccgg gatcgatgtg agagctgtcg
     6181 aacaggggga tgatgcgtgg cacaaattac ggctcggcgt catcaccgct tcagaagttc
     6241 acaacgtgat agcaaaaccc cgctccggaa agaagtggcc tgacatgaaa atgtcctact
     6301 tccacaccct gcttgctgag gtttgcaccg gtgtggctcc ggaagttaac gctaaagcac
     6361 tggcctgggg aaaacagtac gagaacgacg ccagaaccct gtttgaattc acttccggcg
     6421 tgaatgttac tgaatccccg atcatctatc gcgacgaaag tatgcgtacc gcctgctctc
     6481 ccgatggttt atgcagtgac ggcaacggcc ttgaactgaa atgcccgttt acctcccggg
     6541 atttcatgaa gttccggctc ggtggtttcg aggccataaa gtcagcttac atggcccagg
     6601 tgcagtacag catgtgggtg acgcgaaaaa atgcctggta ctttgccaac tatgacccgc
     6661 gtatgaagcg tgaaggcctg cattatgtcg tgattgagcg ggatgaaaag tacatggcga
     6721 gttttgacga gatcgtgccg gagttcatcg aaaaaatgga cgaggcactg gctgaaattg
     6781 gttttgtatt tggggagcaa tggcgatgac gcatcctcac gataataagc ttcctgctga
     6841 acatcaaagg caagaaaaca tctgttgtca aagacagcat ccttgaacaa ggacaattaa
     6901 cagttaacaa ataaaaacgc aaaagaaaat gccgatattg actaccggaa gcagtgtgac
     6961 cgtgtgcttc tcaaatgcct gattcaggct gtctatgtgt gactgttgag ctgtaacaag
     7021 ttgtctcagg tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct
     7081 attaggtgtt acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt
     7141 aaatgcacca aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc
     7201 tgtgcatata gctatactga tttcgtcaga ctcacagtca aacatgccgg tcagttggcc
     7261 tggtgatggc gggatcgttg tatatttctt gacacctttt cggcaccgcc ctaaaattct
     7321 gcgtcctcat aatatatgag gcgatttatt acgtgtttac gaagcaaaag ctaaaaccag
     7381 gagctattta atggataaga aatactcaat aggcttagat atcggcacaa atagcgtcgg
     7441 atgggcggtg atcactgatg aatataaggt tccgtctaaa aagttcaagg ttctgggaaa
     7501 tacagaccgc cacagtatca aaaaaaatct tataggggct cttttatttg acagtggaga
     7561 gacagcggaa gcgactcgtc tcaaacggac agctcgtaga aggtatacac gtcggaagaa
     7621 tcgtatttgt tatctacagg agattttttc aaatgagatg gcgaaagtag atgatagttt
     7681 ctttcatcga cttgaagagt cttttttggt ggaagaagac aagaagcatg aacgtcatcc
     7741 tatttttgga aatatagtag atgaagttgc ttatcatgag aaatatccaa ctatctatca
     7801 tctgcgaaaa aaattggtag attctactga taaagcggat ttgcgcttaa tctatttggc
     7861 cttagcgcat atgattaagt ttcgtggtca ttttttgatt gagggagatt taaatcctga
     7921 taatagtgat gtggacaaac tatttatcca gttggtacaa acctacaatc aattatttga
     7981 agaaaaccct attaacgcaa gtggagtaga tgctaaagcg attctttctg cacgattgag
     8041 taaatcaaga cgattagaaa atctcattgc tcagctcccc ggtgagaaga aaaatggctt
     8101 atttgggaat ctcattgctt tgtcattggg tttgacccct aattttaaat caaattttga
     8161 tttggcagaa gatgctaaat tacagctttc aaaagatact tacgatgatg atttagataa
     8221 tttattggcg caaattggag atcaatatgc tgatttgttt ttggcagcta agaatttatc
     8281 agatgctatt ttactttcag atatcctaag agtaaatact gaaataacta aggctcccct
     8341 atcagcttca atgattaaac gctacgatga acatcatcaa gacttgactc ttttaaaagc
     8401 tttagttcga caacaacttc cagaaaagta taaagaaatc ttttttgatc aatcaaaaaa
     8461 cggatatgca ggttatattg atgggggagc tagccaagaa gaattttata aatttatcaa
     8521 accaatttta gaaaaaatgg atggtactga ggaattattg gtgaaactaa atcgtgaaga
     8581 tttgctgcgc aagcaacgga cctttgacaa cggctctatt ccccatcaaa ttcacttggg
     8641 tgagctgcat gctattttga gaagacaaga agacttttat ccatttttaa aagacaatcg
     8701 tgagaagatt gaaaaaatct tgacttttcg aattccttat tatgttggtc cattggcgcg
     8761 tggcaatagt cgttttgcat ggatgactcg gaagtctgaa gaaacaatta ccccatggaa
     8821 ttttgaagaa gttgtcgata aaggtgcttc agctcaatca tttattgaac gcatgacaaa
     8881 ctttgataaa aatcttccaa atgaaaaagt actaccaaaa catagtttgc tttatgagta
     8941 ttttacggtt tataacgaat tgacaaaggt caaatatgtt actgaaggaa tgcgaaaacc
     9001 agcatttctt tcaggtgaac agaagaaagc cattgttgat ttactcttca aaacaaatcg
     9061 aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa aaaatagaat gttttgatag
     9121 tgttgaaatt tcaggagttg aagatagatt taatgcttca ttaggtacct accatgattt
     9181 gctaaaaatt attaaagata aagatttttt ggataatgaa gaaaatgaag atatcttaga
     9241 ggatattgtt ttaacattga ccttatttga agatagggag atgattgagg aaagacttaa
     9301 aacatatgct cacctctttg atgataaggt gatgaaacag cttaaacgtc gccgttatac
     9361 tggttgggga cgtttgtctc gaaaattgat taatggtatt agggataagc aatctggcaa
     9421 aacaatatta gattttttga aatcagatgg ttttgccaat cgcaatttta tgcagctgat
     9481 ccatgatgat agtttgacat ttaaagaaga cattcaaaaa gcacaagtgt ctggacaagg
     9541 cgatagttta catgaacata ttgcaaattt agctggtagc cctgctatta aaaaaggtat
     9601 tttacagact gtaaaagttg ttgatgaatt ggtcaaagta atggggcggc ataagccaga
     9661 aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact caaaagggcc agaaaaattc
     9721 gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa ttaggaagtc agattcttaa
     9781 agagcatcct gttgaaaata ctcaattgca aaatgaaaag ctctatctct attatctcca
     9841 aaatggaaga gacatgtatg tggaccaaga attagatatt aatcgtttaa gtgattatga
     9901 tgtcgatcac attgttccac aaagtttcct taaagacgat tcaatagaca ataaggtctt
     9961 aacgcgttct gataaaaatc gtggtaaatc ggataacgtt ccaagtgaag aagtagtcaa
    10021 aaagatgaaa aactattgga gacaacttct aaacgccaag ttaatcactc aacgtaagtt
    10081 tgataattta acgaaagctg aacgtggagg tttgagtgaa cttgataaag ctggttttat
    10141 caaacgccaa ttggttgaaa ctcgccaaat cactaagcat gtggcacaaa ttttggatag
    10201 tcgcatgaat actaaatacg atgaaaatga taaacttatt cgagaggtta aagtgattac
    10261 cttaaaatct aaattagttt ctgacttccg aaaagatttc caattctata aagtacgtga
    10321 gattaacaat taccatcatg cccatgatgc gtatctaaat gccgtcgttg gaactgcttt
    10381 gattaagaaa tatccaaaac ttgaatcgga gtttgtctat ggtgattata aagtttatga
    10441 tgttcgtaaa atgattgcta agtctgagca agaaataggc aaagcaaccg caaaatattt
    10501 cttttactct aatatcatga acttcttcaa aacagaaatt acacttgcaa atggagagat
    10561 tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga gaaattgtct gggataaagg
    10621 gcgagatttt gccacagtgc gcaaagtatt gtccatgccc caagtcaata ttgtcaagaa
    10681 aacagaagta cagacaggcg gattctccaa ggagtcaatt ttaccaaaaa gaaattcgga
    10741 caagcttatt gctcgtaaaa aagactggga tccaaaaaaa tatggtggtt ttgatagtcc
    10801 aacggtagct tattcagtcc tagtggttgc taaggtggaa aaagggaaat cgaagaagtt
    10861 aaaatccgtt aaagagttac tagggatcac aattatggaa agaagttcct ttgaaaaaaa
    10921 tccgattgac tttttagaag ctaaaggata taaggaagtt aaaaaagact taatcattaa
    10981 actacctaaa tatagtcttt ttgagttaga aaacggtcgt aaacggatgc tggctagtgc
    11041 cggagaatta caaaaaggaa atgagctggc tctgccaagc aaatatgtga attttttata
    11101 tttagctagt cattatgaaa agttgaaggg tagtccagaa gataacgaac aaaaacaatt
    11161 gtttgtggag cagcataagc attatttaga tgagattatt gagcaaatca gtgaattttc
    11221 taagcgtgtt attttagcag atgccaattt agataaagtt cttagtgcat ataacaaaca
    11281 tagagacaaa ccaatacgtg aacaagcaga aaatattatt catttattta cgttgacgaa
    11341 tcttggagct cccgctgctt ttaaatattt tgatacaaca attgatcgta aacgatatac
    11401 gtctacaaaa gaagttttag atgccactct tatccatcaa tccatcactg gtctttatga
    11461 aacacgcatt gatttgagtc agctaggagg tgactgatgg ctggttggcg tactgttgtg
    11521 gtaaataccc atatggacag ttttcccttt gatatgtaac ggtgaacagt tgttctactt
    11581 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac c
//
