LOCUS       Exported               18656 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 
            cassette to the mouse TIGRE locus.
ACCESSION   .
VERSION     .
KEYWORDS    Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, 
            Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts 
            LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz 
            GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA,
            Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT,
            Sunkin SM, Lecoq J, Lin MZ, Boyden ES, Murphy GJ, da Costa NM, 
            Waters J, Li L, Tasic B, Zeng H
  TITLE     A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced 
            Brain-Cell-Type Targeting and Functionality.
  JOURNAL   Cell. 2018 Jul 12;174(2):465-480.e22. doi: 
            10.1016/j.cell.2018.06.035.
  PUBMED    30007418
REFERENCE   2  (bases 1 to 18656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2959..3040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2984..3003
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    3054..3324
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3357..3395
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3392..3416
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3529..3562
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    3640..3688
                     /note="synthetic polyadenylation signal"
     polyA_signal    3697..4173
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    4185..4646
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    4673..4706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      4717..4726
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4723..6075
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4819..4828
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     5109..5130
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5236..5257)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5497..5516)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    6100..6688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6571..6582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6719..6926
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9455..9758
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9760..10037
                     /label=chicken beta-actin promoter
     intron          10039..11056
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10980..11002
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     11064..11083
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(11144..11177)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     polyA_signal    11215..11263
                     /note="synthetic polyadenylation signal"
     polyA_signal    11272..11748
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(11783..11802)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11827..11875
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(12049..12082)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      12099..12108
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(12238..12256)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12506..12525
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     12700..12723
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             13542..13598
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             13599..14345
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    14388..14976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(14859..14870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15007..15214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    15228..15261
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        15288..15787
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(15323..15346)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     15720..15739
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(16435..16451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16435..16451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16448..16470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16459..16475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(16483..16513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16528..16549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16666..16683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(16837..17425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16917..16936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17596..18456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18219..18238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18457..18561)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     18629..18647
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgtttatt
      481 aagaattcga gctcgcccca tcctcactga ctccgtcctg gagttggatg agagataatg
      541 gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc ttctccaaag
      601 agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc cctggaggtg
      661 atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg tgggtgggtt
      721 ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga cgattgtatg
      781 aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca gctgtatctc
      841 tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac atgcaggctc
      901 agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc tgggttttaa
      961 cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga acgctgtgac
     1021 ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct gtcctgcact
     1081 tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat gtgtcacccc
     1141 agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg gctttacact
     1201 gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga gggtggtgac
     1261 gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc attcaaggcc
     1321 aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca catagcaggg
     1381 ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat gattctgtca
     1441 ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc
     1501 tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg
     1561 ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc
     1621 cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct
     1681 ttgggggggg gctgtcccga gctcggtacc gccccatcct cactgactcc gtcctggagt
     1741 tggatgagag ataatggcct tacgttgtgc caggggaggg tcgggctgga tttagcaaga
     1801 tttaccttct ccaaagagcg gtgctgcagt ggcacagctg cccacggagg tgggggggtc
     1861 accgtccctg gaggtgatga agaactgtgg ggatgtggca ctgagggaca tggccagtgg
     1921 gcacggtggg tgggttgggg ttggtcttgg ggatcttgga gggcttttcc agccttcatg
     1981 atttgacgat tgtatgaaca tctacatggc aattctccag ctgcctgtcc cagtcctact
     2041 gacccagctg tatctctcca ggcaagctct tccacccctt ctgcttgcat ccagacacca
     2101 tcaaacatgc aggctcagac acagggacca gcagtgtctg tggccttttt gtgctcctct
     2161 ccatgctggg ttttaacttg ctctttgtcc ttctatccta tcttcttatc cttaaggctg
     2221 ttctgaacgc tgtgacttgg agagtgtccc agagccctca acacctgcat gtcccacgtc
     2281 catgctgtcc tgcacttcct tatccccaag atctgcctct ccgtgatgca ctgaattggc
     2341 aaacatgtgt caccccagac caacaatgtc acagcaaact cccccttgat aggacaaggg
     2401 ggaatggctt tacactgaga caggggaggt ttgggttgga tatgaggagg cagtttttcc
     2461 cccagagggt ggtgacgcac tgaacaggtt gcccaaggag gctgtggatg ccccatccct
     2521 gcaggcattc aaggccaggc tggatgtggc tctgggcagc ctgggctgct ggttgatgac
     2581 cctgcacata gcagggggtt ggatctggat gagcactgtg ctcctttgca acccaggccg
     2641 ttctatgatt ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc
     2701 ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc
     2761 accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc
     2821 cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat
     2881 tacatccctg ggggctttgg gggggggctg tcccggtacc cggggatcca ccggtatcga
     2941 ttcggccggc cgacgtcggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3001 tgctttattt gtaaccatta taagctgcaa taaacaagtt tcgagtttac cactccctat
     3061 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3121 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     3181 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3241 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3301 accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg ggtcgaggta
     3361 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3421 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     3481 gcggccccga attcgagctc ggtacccggg gatcaaattc aattaaatat aacttcgtat
     3541 agcatacatt atacgaagtt attcgcgagt gaataaatga aagcttgcag atcccctcag
     3601 caccatggct agcggcagcc tcggagtttg aatagataga ataaaatatc tttattttca
     3661 ttccatctgt gtgttggttt tttgtgtgag atctacgggt ggcatccctg tgacccctcc
     3721 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3781 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3841 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3901 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3961 gttcaagcga ttctcctgcc tcaggctccc gagttgttgg gattccaggc atgcatgacc
     4021 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4081 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     4141 caggcgtgaa ccactgctcc cttccctgtc cttctgcctc agcgctgtaa gtctgcagaa
     4201 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact
     4261 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg
     4321 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg
     4381 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg
     4441 gccagctcat tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt
     4501 ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc
     4561 atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt
     4621 tttgccaagt tctaattcca tcagaaagct tttaattaac tgaatagcta acataacttc
     4681 gtatagcata cattatacga agttatcacg cgtgccgcca ccatgggttc tcatcatcat
     4741 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
     4801 gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg gaataagaca
     4861 ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta tatcaaggcc
     4921 gacaagcaga agaacggcat caaggcgaac ttccacatcc gccacaacat cgaggacggc
     4981 ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5041 ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc caacgagaag
     5101 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5161 gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga gctgttcacc
     5221 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     5281 tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     5341 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     5401 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     5461 gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     5521 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5581 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc ggaccaactg
     5641 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
     5701 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
     5761 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
     5821 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
     5881 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
     5941 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     6001 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     6061 atgacagcga agtaaacgcg ttacgtaaag cttgtcgata atcaacctct ggattacaaa
     6121 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6181 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     6241 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     6301 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     6361 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6421 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6481 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc cacctggatt
     6541 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6601 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6661 cggatctccc tttgggccgc ctccccgcat cgacaccgag cgctgctcga ggcctcgact
     6721 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6781 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6841 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6901 gaagacaata gcaggcatgc tggggacacg tgggatcctc tagtctagag ccccatcctc
     6961 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
     7021 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
     7081 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
     7141 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
     7201 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
     7261 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
     7321 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
     7381 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     7441 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     7501 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     7561 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     7621 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     7681 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     7741 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     7801 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     7861 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     7921 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     7981 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     8041 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     8101 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt ccctctagag
     8161 ccccatcctc actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc
     8221 aggggagggt cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg
     8281 gcacagctgc ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg
     8341 gatgtggcac tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg
     8401 gatcttggag ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca
     8461 attctccagc tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt
     8521 ccaccccttc tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag
     8581 cagtgtctgt ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct
     8641 tctatcctat cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca
     8701 gagccctcaa cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga
     8761 tctgcctctc cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca
     8821 cagcaaactc ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt
     8881 tgggttggat atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg
     8941 cccaaggagg ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct
     9001 ctgggcagcc tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg
     9061 agcactgtgc tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt
     9121 tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag
     9181 aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg
     9241 ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct
     9301 gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt
     9361 ccctctagag tcgacggcgc gccctgcagg aatctagtta ttaatagtaa tcaattacgg
     9421 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     9481 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     9541 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     9601 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     9661 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     9721 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
     9781 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     9841 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
     9901 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
     9961 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
    10021 gaagcgcgcg gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc
    10081 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
    10141 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
    10201 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
    10261 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
    10321 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
    10381 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
    10441 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
    10501 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
    10561 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
    10621 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg
    10681 cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg
    10741 gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc
    10801 tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag
    10861 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct
    10921 ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg
    10981 gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt
    11041 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg
    11101 caaagaattg atttgatacc gcgggccccg cgtggacggt accataactt cgtataaagt
    11161 atcctatacg aagttatcca tggctagcgg cagcctcgga gtttgaatag atagaataaa
    11221 atatctttat tttcattcca tctgtgtgtt ggttttttgt gtgagatcta cgggtggcat
    11281 ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc agtgcccacc
    11341 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
    11401 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
    11461 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
    11521 atctccgcct cctgggttca agcgattctc ctgcctcagg ctcccgagtt gttgggattc
    11581 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
    11641 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
    11701 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gcctcagcta
    11761 attgagtagg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
    11821 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
    11881 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    11941 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    12001 gggctcgcag ccaacgtcgg ggcggcaggc ctcgcgaggg tttctagaat aacttcgtat
    12061 aaagtatcct atacgaagtt atacgcgcgc gtactagcgc caccatggtg agcaagggcg
    12121 aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc
    12181 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
    12241 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
    12301 tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc
    12361 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg
    12421 tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc
    12481 gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg
    12541 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc
    12601 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga
    12661 agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca
    12721 acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac cacctgttcc
    12781 tggggcatgg taccggcagc accggcagcg gcagctccgg caccgcctcc tccgaggaca
    12841 acaacatggc cgtgattaag gagtttatga ggtttaaagt caggatggag ggcagcatga
    12901 acgggcacga gtttgagatt gaaggagagg gggaagggag gccctatgag ggcacccaga
    12961 cagcaaagct gaaagtgaca aagggcggcc ctctgccatt cgcatgggac atcctgtctc
    13021 ctcagtttat gtatggaagt aaagcctacg tgaaacaccc tgctgacatt ccagattaca
    13081 agaaactgag cttcccagag gggtttaagt gggaaagagt gatgaacttc gaagacgggg
    13141 gcctggtgac tgtcacccag gacagctctc tgcaggacgg cactctgatc tataaggtga
    13201 aaatgcgcgg caccaatttt ccccctgatg gcccagtcat gcagaagaag acaatgggct
    13261 gggaggcttc aacagaaagg ctgtacccca gagacggcgt gctgaaggga gagatccacc
    13321 aggcactgaa gctgaaagat ggagggcatt atctggtcga attcaaaacc atctacatgg
    13381 ccaagaaacc cgtgcagctg cctggctact attacgtcga cacaaagctg gatattacta
    13441 gccataacga agactacacc attgtggagc agtatgagcg gagcgagggg aggcaccatc
    13501 tgtttctgta cggcatggac gagctgtaca agggaagcgg agctactaac ttcagcctgc
    13561 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gtctagactg gacaagagca
    13621 aagtcataaa ctctgctctg gaattactca atgaagtcgg tatcgaaggc ctgacgacaa
    13681 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
    13741 agcgggccct gctcgatgcc ctggcaatcg agatgctgga caggcatcat acccacttct
    13801 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct
    13861 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
    13921 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
    13981 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
    14041 atcaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
    14101 cacttctgag acaagcaatt gagctgttcg accatcaggg agccgaacct gccttccttt
    14161 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
    14221 cggccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
    14281 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
    14341 ggtaactaag taaggatcaa atggcgcgtt acgtaaagct tgtcgataat caacctctgg
    14401 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
    14461 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
    14521 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
    14581 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
    14641 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
    14701 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
    14761 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca
    14821 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
    14881 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
    14941 agacgagtcg gatctccctt tgggccgcct ccccgcatcg acaccgagcg ctgctcgagg
    15001 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    15061 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    15121 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    15181 aggattggga agacaatagc aggcatgctg gggacacgtg ggatccggtg ccagggcgtg
    15241 cccttgggct ccccgggcgc gtttgggcgg ccgcatcgaa ttctaccggg taggggaggc
    15301 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
    15361 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
    15421 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
    15481 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
    15541 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    15601 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    15661 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
    15721 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    15781 cctttcgacc tgcaatcgcc gctagccgcc accatgaaaa agcctgaact caccgcgacg
    15841 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
    15901 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
    15961 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
    16021 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
    16081 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
    16141 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
    16201 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
    16261 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
    16321 gtcagtgtaa gtgctagccc ctaggcctga atcgaagttc ctattcttca aaaggtatag
    16381 gaacttcgag ctcgtcgact gcagaggcct gcatgcaagc ttggcgtaat catggtcata
    16441 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    16501 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    16561 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    16621 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    16681 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    16741 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    16801 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    16861 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    16921 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    16981 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    17041 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    17101 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    17161 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    17221 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    17281 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    17341 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    17401 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    17461 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    17521 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    17581 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    17641 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    17701 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    17761 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    17821 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    17881 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    17941 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    18001 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    18061 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    18121 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    18181 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    18241 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    18301 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    18361 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    18421 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    18481 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    18541 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    18601 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
