LOCUS Exported 2874 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION 4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard. ACCESSION . VERSION . KEYWORDS pUC19_4xT790M_28bp SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2874) AUTHORS Hayward SL, Lund PE, Kang Q, Johnson-Buck A, Tewari M, Walter NG TITLE Ultra-specific and Amplification-free Quantification of Mutant DNA by Single-molecule Kinetic Fingerprinting. JOURNAL J Am Chem Soc. 2018 Aug 20. doi: 10.1021/jacs.8b06685. PUBMED 30125495 REFERENCE 2 (bases 1 to 2874) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2874 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(95..955) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 718..737 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(956..1060) /gene="bla" /label=AmpR promoter primer_bind 1128..1146 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(1184..1206) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1306..1325 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1519..1541 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1533..1550 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1534..1550 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1808..1824) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1808..1824) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1821..1843) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1832..1848 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1856..1886) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1901..1922 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2039..2056) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2210..2798) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2290..2309) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 61 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 121 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 181 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 241 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 301 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 361 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 421 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 481 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 541 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 601 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 661 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 721 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 781 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 841 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 901 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 961 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 1021 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 1081 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 1141 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1201 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1261 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 1321 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1381 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 1441 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 1501 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc 1561 tcggtaccca gcgagtcatt caatcatgca gctcatgccc ttcgtaggac gtcaaagtcg 1621 agtccgacaa tcatgcagct catgcccttc gtaggacgtc aagttggagt cagtcaatca 1681 tgcagctcat gcccttcgta ggacgtcaaa gtcgagtccg acaatcatgc agctcatgcc 1741 cttcgtagga cgtctaaggg gatcctctag agtcgacctg caggcatgca agcttggcgt 1801 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 1861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 1921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 1981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 2101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2641 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atga //