LOCUS Exported 5406 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Membrane, Excitation: 580, Emission: 608. ACCESSION . VERSION . KEYWORDS FusionRed-Dectin1A-N-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5406) AUTHORS Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM, Shcherbo D TITLE A monomeric red fluorescent protein with low cytotoxicity. JOURNAL Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208. PUBMED 23149748 REFERENCE 2 (bases 1 to 5406) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5406 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 537..546 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 1308..1317 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1314..2012 /codon_start=1 /product="monomeric red fluorescent protein optimized for use as a fusion tag (Shemiakina et al., 2012)" /label=FusionRed /note="mammalian codon-optimized" /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY STGGAGDGGK" polyA_signal 2134..2255 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2171..2190) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2225..2244 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2262..2717) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2399..2420) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2611..2630 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2744..2848 /gene="bla" /label=AmpR promoter promoter 2850..3207 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2873..2893) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3058..3193 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3120..3139 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3242..4036 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3296..3315) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3906..3925 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4224..4243) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4268..4315 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4644..5232 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5133..5152 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 541 ccatggaata tcatcctgat ttagaaaatt tggatgaaga tggatatact caattacact 601 tcgactctca aagcaatacc aggatagctg ttgtttcaga gaaaggatcg tgtgctgcat 661 ctcctccttg gcgcctcatt gctgtaattt tgggaatcct atgcttggta atactggtga 721 tagctgtggt cctgggtacc atggctattt ggagatccaa ttcaggaagc aacacattgg 781 agaatggcta ctttctatca agaaataaag agaaccacag tcaacccaca caatcatctt 841 tagaagacag tgtgactcct accaaagctg tcaaaaccac aggggttctt tccagccctt 901 gtcctcctaa ttggattata tatgagaaga gctgttatct attcagcatg tcactaaatt 961 cctgggatgg aagtaaaaga caatgctggc aactgggctc taatctccta aagatagaca 1021 gctcaaatga attgggattt atagtaaaac aagtgtcttc ccaacctgat aattcatttt 1081 ggataggcct ttctcggccc cagactgaag taccatggct ctgggaggat ggatcaacat 1141 tctcttctaa cttatttcag atcagaacca cagctaccca agaaaaccca tctccaaatt 1201 gtgtatggat tcacgtgtca gtcatttatg accaactgtg tagtgtgccc tcatatagta 1261 tttgtgagaa gaagttttca atgagctccg gaggggatcc accggtcgcc accatggtga 1321 gcgagctgat taaggagaac atgcccatga agctgtacat ggagggcacc gtgaacaacc 1381 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga 1441 gaatcaaggt cgtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct 1501 tcatgtacgg cagcagaacc ttcatcaagc accctccggg catccccgac ttctttaagc 1561 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc 1621 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaaggtta 1681 gaggggtgaa cttcccagcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg 1741 cctccaccga gacgatgtac cccgctgacg gcggcctgga aggcgcatgt gacatggccc 1801 tgaagctcgt gggcgggggc cacctgatct gcaaccttga gaccacatac agatccaaga 1861 aacccgctac gaacctcaag atgcccggcg tctacaacgt ggaccacaga ctggaaagaa 1921 tcaaggaggc cgacgatgag acctacgtcg agcagcacga ggtggctgtg gccagatact 1981 ctactggtgg cgctggtgat ggaggtaaat gagcggccgc gactctagat cataatcagc 2041 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 2101 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 2161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2221 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat 2281 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2341 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 2401 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 2461 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 2521 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 2581 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 2641 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 2701 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2821 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 2881 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 2941 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 3001 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 3061 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 3121 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 3181 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 3241 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3301 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3361 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 3421 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 3481 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 3541 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 3601 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 3661 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 3721 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 3781 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 3841 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 3901 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 3961 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4021 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 4081 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 4141 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 4201 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 4261 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 4321 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 4381 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 4441 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 4501 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 4561 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4621 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4681 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4741 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4801 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4861 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4921 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4981 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5041 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5101 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5161 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5221 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5281 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5341 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 5401 gttccg //