LOCUS       Exported                6379 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Localization: Focal Adhesions, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    mIFP12-ILK-C-14
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1512
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     polyA_signal    3107..3228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3144..3163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3198..3217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3235..3690)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3372..3393)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3584..3603
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3717..3821
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3823..4180
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(3846..3866)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4031..4166
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4093..4112
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4215..5009
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4269..4288)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4879..4898
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5197..5216)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5241..5288
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5617..6205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6106..6125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgtcggt accgctgact acctcagcat tcggccacgc gtttctggct
      601 aactgtgaac gcgagcagat ccacctggcg ggctccattc agccgcacgg tatcctgctg
      661 gctgtgaaag agccggacaa cgtggtgatc caggcttcta ttaacgctgc ggagttcctg
      721 aacaccaact ctgttgttgg ccgtccgctg cgtgacctgg gcggcgatct gcctttgcag
      781 atcctgccgc acctgaacgg cccgctgcac ctggctccga tgaccctgcg ttgtaccgtg
      841 ggttctccgc cgcgtcgtgt ggactgtacc attcatcgtc cgtctaacgg cggcctgatc
      901 gtagaactgg aaccagcaac caagaccact aacattgcgc cggctctgga cggtgcgttt
      961 catcgtatca cttcttcatc ctccctgatg ggcctgtgtg acgaaaccgc gactattatc
     1021 cgtgagatta ctggctacga ccgtgtgatg gtagtacgtt tcgatgaaga gggtaatggc
     1081 gaaattctgt ccgaacgtcg tcgtgcggac ctggaagcgt tcctgggtaa ccgctacccg
     1141 gcgtctacta ttccgcagat cgctcgtcgc ctgtacgaac ataaccgtgt tcgcctgctg
     1201 gtagatgtga actatactcc ggttccgcta cagccgcgca tcagcccgct gaacggtcgt
     1261 gatctggata tgtccctgtc ttgcctgcgc tctatgtccc cgatccacca gaaatacatg
     1321 caggacatgg gcgttggcgc gaccctggtt tgctctctga tggtgtctgg tcgtctgtgg
     1381 ggtctgatcg cttgccacca ctacgaaccg cgcttcgttc cgttccacat tcgcgctgct
     1441 ggcgaagcgc tggcggaaac ttgtgcgatc cgcatcgcga cgctggagag ctttgcacag
     1501 tctcagtcca aaggtggcgg aagcggcggc ggaagcggtg gcggaagttc cggactcaga
     1561 tctcgaggaa gcggcggatc tgggagtggc gacgacattt tcactcagtg ccgggagggc
     1621 aacgcagtcg ccgttcgcct gtggctggac aacacggaga acgacctcaa ccagggggac
     1681 gatcatggct tctccccctt gcactgggcc tgccgagagg gccgctctgc tgtggttgag
     1741 atgttgatca tgcggggggc acggatcaat gtaatgaacc gtggggatga cacccccctg
     1801 catctggcag ccagtcatgg acaccgtgat attgtacaga agctattgca gtacaaggca
     1861 gacatcaatg cagtgaatga acatgggaat gtgcccctgc actatgcctg tttttggggc
     1921 caagatcaag tggcagagga cctggtggca aatggggccc ttgtcagcat ctgtaacaag
     1981 tatggagaga tgcctgtgga caaagccaag gcacccctga gagagcttct ccgagagcgg
     2041 gcagagaaga tgggccagaa tctcaaccgt attccataca aggacacatt ctggaagggg
     2101 accacccgca ctcggccccg aaatggaacc ctgaacaaac actctggcat tgacttcaaa
     2161 cagcttaact tcctgacgaa gctcaacgag aatcactctg gagagctatg gaagggccgc
     2221 tggcagggca atgacattgt cgtgaaggtg ctgaaggttc gagactggag tacaaggaag
     2281 agcagggact tcaatgaaga gtgtccccgg ctcaggattt tctcgcatcc aaatgtgctc
     2341 ccagtgctag gtgcctgcca gtctccacct gctcctcatc ctactctcat cacacactgg
     2401 atgccatatg gatccctcta caatgtacta catgaaggca ccaatttcgt cgtggaccag
     2461 agccaggctg tgaagtttgc tttggacatg gcaaggggca tggccttcct acacacacta
     2521 gagcccctca tcccacgaca tgcactcaat agccgtagtg taatgattga tgaggacatg
     2581 actgcccgaa ttagcatggc tgatgtcaag ttctctttcc aatgtcctgg tcgcatgtat
     2641 gcacctgcct gggtagcccc cgaagctctg cagaagaagc ctgaagacac aaacagacgc
     2701 tcagcagaca tgtggagttt tgcagtgctt ctgtgggaac tggtgacacg ggaggtaccc
     2761 tttgctgacc tctccaatat ggagattgga atgaaggtgg cattggaagg ccttcggcct
     2821 accatcccac caggtatttc ccctcatgtg tgtaagctca tgaagatctg catgaatgaa
     2881 gaccctgcaa agcgacccaa atttgacatg attgtgccta tccttgagaa gatgcaggac
     2941 aagtaggaat tctgcagtcg acggtaccgc gggcccggga tccaccggat ctagataact
     3001 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3061 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcgtaaattg
     3241 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3301 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3361 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     3421 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     3481 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     3541 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     3601 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     3661 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     3721 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3781 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     3841 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3901 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4021 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4081 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4141 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     4201 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4261 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4321 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4381 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4441 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4501 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4561 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4621 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4681 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     4741 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     4801 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     4861 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     4921 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     4981 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     5041 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     5101 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     5161 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     5221 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     5281 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     5341 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     5401 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     5461 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5521 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5581 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5641 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5701 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5761 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5821 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5881 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5941 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6001 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6061 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6121 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6181 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6241 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6301 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     6361 gcccatatat ggagttccg
//
