LOCUS Exported 10346 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants. ACCESSION . VERSION . KEYWORDS pEPOR1CB0002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10346) AUTHORS Raitskin O, Schudoma C, West A, Patron NJ TITLE Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering. JOURNAL PLoS One. 2019 Feb 27;14(2):e0211598. doi: 10.1371/journal.pone.0211598. eCollection 2019. PUBMED 30811422 REFERENCE 2 (bases 1 to 10346) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10346 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 438..783 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 726..746 /label=35S promoter /note="CaMV 35S promoter, forward primer" CDS 874..4977 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 4990..5010 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5017..5736 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(5062..5083) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5323..5342) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5670..5691 /label=EGFP-C /note="EGFP, forward primer" terminator 5749..6001 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" misc_feature 6049..6073 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS complement(6229..7377) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" rep_origin complement(7713..8301) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7793..7812) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8472..9332) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9095..9114 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9333..9437) /gene="bla" /label=AmpR promoter rep_origin 9524..10155 /label=oriV /note="incP origin of replication" misc_feature 10247..10271 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 tctgtgaaga caagcaagaa ttcaagcttg gaggtcaaca tggtggagca cgacactctg 61 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt 121 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc 181 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga 241 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg 301 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt 361 gataacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca 421 gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga 481 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc 541 tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt 601 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc 661 acgtctacaa agcaagtgga ttgatgtgac atctccactg acgtaaggga tgacgcacaa 721 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg 781 acacgctcga gtataagagc tcatttttac aacaattacc aacaacaaca aacaacaaac 841 aacattacaa ttacatttac aattatcgat acaatggata agaagtactc tatcggactc 901 gatatcggaa ctaactctgt gggatgggct gtgatcaccg atgagtacaa ggtgccatct 961 aagaagttca aggttctcgg aaacaccgat aggcactcta tcaagaaaaa ccttatcggt 1021 gctctcctct tcgattctgg tgaaactgct gaggctacca gactcaagag aaccgctaga 1081 agaaggtaca ccagaagaaa gaacaggatc tgctacctcc aagagatctt ctctaacgag 1141 atggctaaag tggatgattc attcttccac aggctcgaag agtcattcct cgtggaagaa 1201 gataagaagc acgagaggca ccctatcttc ggaaacatcg ttgatgaggt ggcataccac 1261 gagaagtacc ctactatcta ccacctcaga aagaagctcg ttgattctac tgataaggct 1321 gatctcaggc tcatctacct cgctctcgct cacatgatca agttcagagg acacttcctc 1381 atcgagggtg atctcaaccc tgataactct gatgtggata agttgttcat ccagctcgtg 1441 cagacctaca accagctttt cgaagagaac cctatcaacg cttcaggtgt ggatgctaag 1501 gctatcctct ctgctaggct ctctaagtca agaaggcttg agaacctcat tgctcagctc 1561 cctggtgaga agaagaacgg acttttcgga aacttgatcg ctctctctct cggactcacc 1621 cctaacttca agtctaactt cgatctcgct gaggatgcaa agctccagct ctcaaaggat 1681 acctacgatg atgatctcga taacctcctc gctcagatcg gagatcagta cgctgatttg 1741 ttcctcgctg ctaagaacct ctctgatgct atcctcctca gtgatatcct cagagtgaac 1801 accgagatca ccaaggctcc actctcagct tctatgatca agagatacga tgagcaccac 1861 caggatctca cacttctcaa ggctcttgtt agacagcagc tcccagagaa gtacaaagag 1921 attttcttcg atcagtctaa gaacggatac gctggttaca tcgatggtgg tgcatctcaa 1981 gaagagttct acaagttcat caagcctatc ctcgagaaga tggatggaac cgaggaactc 2041 ctcgtgaagc tcaatagaga ggatcttctc agaaagcaga ggaccttcga taacggatct 2101 atccctcatc agatccacct cggagagttg cacgctatcc ttagaaggca agaggatttc 2161 tacccattcc tcaaggataa cagggaaaag attgagaaga ttctcacctt cagaatccct 2221 tactacgtgg gacctctcgc tagaggaaac tcaagattcg cttggatgac cagaaagtct 2281 gaggaaacca tcaccccttg gaacttcgaa gaggtggtgg ataagggtgc tagtgctcag 2341 tctttcatcg agaggatgac caacttcgat aagaaccttc caaacgagaa ggtgctccct 2401 aagcactctt tgctctacga gtacttcacc gtgtacaacg agttgaccaa ggttaagtac 2461 gtgaccgagg gaatgaggaa gcctgctttt ttgtcaggtg agcaaaagaa ggctatcgtt 2521 gatctcttgt tcaagaccaa cagaaaggtg accgtgaagc agctcaaaga ggattacttc 2581 aagaaaatcg agtgcttcga ttcagttgag atttctggtg ttgaggatag gttcaacgca 2641 tctctcggaa cctaccacga tctcctcaag atcattaagg ataaggattt cttggataac 2701 gaggaaaacg aggatatctt ggaggatatc gttcttaccc tcaccctctt tgaagataga 2761 gagatgattg aagaaaggct caagacctac gctcatctct tcgatgataa ggtgatgaag 2821 cagttgaaga gaagaagata cactggttgg ggaaggctct caagaaagct cattaacgga 2881 atcagggata agcagtctgg aaagacaatc cttgatttcc tcaagtctga tggattcgct 2941 aacagaaact tcatgcagct catccacgat gattctctca cctttaaaga ggatatccag 3001 aaggctcagg tttcaggaca gggtgatagt ctccatgagc atatcgctaa cctcgctgga 3061 tctcctgcaa tcaagaaggg aatcctccag actgtgaagg ttgtggatga gttggtgaag 3121 gtgatgggaa ggcataagcc tgagaacatc gtgatcgaaa tggctagaga gaaccagacc 3181 actcagaagg gacagaagaa ctctagggaa aggatgaaga ggatcgagga aggtatcaaa 3241 gagcttggat ctcagatcct caaagagcac cctgttgaga acactcagct ccagaatgag 3301 aagctctacc tctactacct ccagaacgga agggatatgt atgtggatca agagttggat 3361 atcaacaggc tctctgatta cgatgttgat catatcgtgc cacagtcatt cttgaaggat 3421 gattctatcg ataacaaggt gctcaccagg tctgataaga acaggggtaa gagtgataac 3481 gtgccaagtg aagaggttgt gaagaaaatg aagaactatt ggaggcagct cctcaacgct 3541 aagctcatca ctcagagaaa gttcgataac ttgactaagg ctgagagggg aggactctct 3601 gaattggata aggcaggatt catcaagagg cagcttgtgg aaaccaggca gatcactaag 3661 cacgttgcac agatcctcga ttctaggatg aacaccaagt acgatgagaa cgataagttg 3721 atcagggaag tgaaggttat caccctcaag tcaaagctcg tgtctgattt cagaaaggat 3781 ttccaattct acaaggtgag ggaaatcaac aactaccacc acgctcacga tgcttacctt 3841 aacgctgttg ttggaaccgc tctcatcaag aagtatccta agctcgagtc agagttcgtg 3901 tacggtgatt acaaggtgta cgatgtgagg aagatgatcg ctaagtctga gcaagagatc 3961 ggaaaggcta ccgctaagta tttcttctac tctaacatca tgaatttctt caagaccgag 4021 attaccctcg ctaacggtga gatcagaaag aggccactca tcgagacaaa cggtgaaaca 4081 ggtgagatcg tgtgggataa gggaagggat ttcgctaccg ttagaaaggt gctctctatg 4141 ccacaggtga acatcgttaa gaaaaccgag gtgcagaccg gtggattctc taaagagtct 4201 atcctcccta agaggaactc tgataagctc attgctagga agaaggattg ggaccctaag 4261 aaatacggtg gtttcgattc tcctaccgtg gcttactctg ttctcgttgt ggctaaggtt 4321 gagaagggaa agagtaagaa gctcaagtct gttaaggaac ttctcggaat cactatcatg 4381 gaaaggtcat ctttcgagaa gaacccaatc gatttcctcg aggctaaggg atacaaagag 4441 gttaagaagg atctcatcat caagctccca aagtactcac tcttcgaact cgagaacggt 4501 agaaagagga tgctcgcttc tgctggtgag cttcaaaagg gaaacgagct tgctctccca 4561 tctaagtacg ttaactttct ttacctcgct tctcactacg agaagttgaa gggatctcca 4621 gaagataacg agcagaagca acttttcgtt gagcagcaca agcactactt ggatgagatc 4681 atcgagcaga tctctgagtt ctctaaaagg gtgatcctcg ctgatgcaaa cctcgataag 4741 gtgttgtctg cttacaacaa gcacagagat aagcctatca gggaacaggc agagaacatc 4801 atccatctct tcacccttac caacctcggt gctcctgctg ctttcaagta cttcgataca 4861 accatcgata ggaagagata cacctctacc aaagaagtgc tcgatgctac cctcatccat 4921 cagtctatca ctggactcta cgagactagg atcgatctct cacagctcgg tggtgattca 4981 agggctgatc ctaagaagaa gaggaaggtt ggttcgatgg tgagcaaggg cgaggagctg 5041 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 5101 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 5161 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 5221 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 5281 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 5341 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 5401 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 5461 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 5521 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 5581 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg 5641 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 5701 gggatcactc tcggcatgga cgagctgtac aagtgagctt gtcaagcaga tcgttcaaac 5761 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata 5821 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt 5881 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac 5941 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat 6001 cgacgctact attgtcttct gcacgaagtg gtttaaacta tcagtgtttg acaggatata 6061 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc 6121 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga 6181 tcaggcgctg gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca 6241 atgcaccagg tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc 6301 gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat 6361 gaggcggaag gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa 6421 catccgtcgg gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg 6481 gtcgccagca aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt 6541 cttgccacgg tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac 6601 gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt 6661 cgtgtaatac cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa 6721 ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag 6781 ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac 6841 gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa 6901 cagggcagag cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat 6961 atcgaacaag gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc 7021 ctgcttggcc tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt 7081 cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc 7141 aagacgacgc gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg 7201 cacgctgtcg cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa 7261 ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga 7321 aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca 7381 ccctccttgc gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga 7441 cccgcctggt gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta 7501 gaccgtctgg ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag 7561 cgaccccttg cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg 7621 gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggatctgcc 7681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 8041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 8101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 8161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 8221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 8281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8581 ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta 8641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 9001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 9061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 9121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 9181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9361 atttatcaag gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9421 caaatagggg ttccgcgcac gaattggcca gcgctgccat ttttggggtg aggccgttcg 9481 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 9541 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 9601 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 9661 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 9721 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 9781 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 9841 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 9901 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 9961 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 10021 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 10081 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 10141 agcccggtga gcgtcgcaaa ggagatcctg atctgactga tgggctgcct gtatcgagtg 10201 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt 10261 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt 10321 actggggtgg atgcagtggg ccccac //