LOCUS       Exported                 775 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    p5xFbx15 (-544/-527)-TK-Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Tokuzawa Y, Kaiho E, Maruyama M, Takahashi K, Mitsui K, Maeda M, 
            Niwa H, Yamanaka S
  TITLE     Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic 
            stem cell self-renewal and mouse development.
  JOURNAL   Mol Cell Biol. 2003 Apr . 23(8):2699-708.
  PUBMED    12665572
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      296..305
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(368..386)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttctctatcg ataggtaccg agctcttacg cgtttatcat aacaatggtt tatcataaca
       61 atggtttatc ataacaatgg tttatcataa caatggttta tcataacaat gggatctgcc
      121 gccccgactg catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg
      181 tcatcataga actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg
      241 agcaacgggc cacggggatg aagcagaagc ttggcattcc ggtactgttg gtaaagccac
      301 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      361 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      421 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      481 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      541 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      601 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      661 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      721 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attac
//
